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| 名称 | LN-18 (人胶质母细胞瘤) (STR鉴定正确) |
| 别称 | LN 18; LN18; LN018 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:4-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | LN-18 is a cell line exhibiting epithelial-like morphology that was isolated in 1976 from the right temporal lobe of a White, 65-year-old, male patient with glioblastoma. The cells are negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner. Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. |
| 年龄（性别） | 男性；65岁 |
| 组织来源 | 大脑；右颞叶 |
| 肿瘤类型 | 肿瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 72 hours (PubMed=7211194); 33 hours (PubMed=9842975) |
| 致瘤性 | Yes; Yes, forms tumors in nude mice |
| 抗原表达情况 | HLA A2, A9, B5, BW35, DRW3 |
| 基因表达情况 | fibronectin |
| 保藏机构 | ATCC; CRL-2610 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 12 D2S1338 17 D3S1358 15,16 D5S818 11,13 D7S820 8,10 D8S1179 12,14 D13S317 12,13 D16S539 11,13 D18S51 17,19 D19S433 12,15 D21S11 28 FGA 19,23 PentaD 11 PentaE 7,10 TH01 9 TPOX 8 vWA 17,18 D6S1043 12,19 D12S391 17,19 D2S441 15 -
STR鉴定图
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