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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Human

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EGF/Urogastrone/URG, Human, Recombinant

货号:PCK001

价格:¥400¥780¥1280¥1880

规格:
10μg
  • 10μg
  • 50μg
  • 500μg
  • 1mg
数量: - +
产品概述

产品信息

别名 Urogastrone, URG
物种 Human
表达宿主 E.coli
序列信息 MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus
检索号 P01133.2
分子量 7.16 kDa
表观分子量 9-11 kDa
标签 His-tag at the C-terminus
生物活性 Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 10^6 IU/mg.

产品特性

纯度 >95% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素 <0.1 EU per 1 μg of the protein by the LAL method.
保存 Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
运输 Ambient temperature or ice pack.
制剂 The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
复融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介绍

Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
参考文献
文献列表
  • Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome(2022/09/13)

    作者:Tianli Shen, Yunhua Wu, Xingjie Wang

    期刊:EXPERIMENTAL AND MOLECULAR MEDICINE

    DOI:10.1038/s12276-022-00848-3

    影响因子 :12.800

    引用产品:CL-0666 PCK001

  • Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer(2022/11/27)

    作者:Tan, Bo, Zhang, Jingwei, Wang, Wen, Ma, Haibo, Yang, Yuanyuan

    期刊:APOPTOSIS

    DOI:10.1007/s10495-022-01789-y

    影响因子 :7.200

    引用产品:CL-0016 CL-0165 CL-0298 CL-0668 CM-0496 PCK001 PM150110

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