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产品信息
| 名称 | HBE135-E6E7 (人支气管上皮细胞) (STR鉴定正确) |
| 别称 | HBE 135-E6E7; HBE 135-E6/E7 |
| 背景描述 | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/mL G418 |
| 种属 | 人 |
| 年龄（性别） | 男性, 54岁 |
| 组织来源 | 肺；支气管 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样；卵圆形 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | 2 |
| 生长培养基 | KSFM+5 ng/ml rhEGF + 0.05 mg/ml BPE0.05 mg/ml BPE(Bovine Pituitary Extract)+0.005 mg/ml insulin +500 ng/ml hydrocortisone |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 倍增时间 | Approximately 24 to 30 hrs |
| 冻存条件 | 冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 保藏机构 | ATCC; CRL-2741 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 9 D2S1338 16,17 D3S1358 15,17 D5S818 12 D7S820 9,10 D8S1179 13,14 D13S317 8,11 D16S539 9,12 D18S51 12 D19S433 13,16 D21S11 28,30 FGA 24,25 PentaD 9,12 PentaE 12,16 TH01 5,6 TPOX 8,10 vWA 16,17 D6S1043 13 D12S391 19,24 D2S441 10,11 -
STR鉴定图
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