IL-22, Mouse, Recombinant
英文名称：IL-22, Mouse, Recombinant
货号： PCK255
价格： ￥14680 ￥3480 ￥1180 ￥19680
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 别名 | Interleukin-22;IL-22;IL-10-Related T-Cell-Derived-Inducible Factor;IL-TIF;IL-TIF Alpha;Interleukin-22a;IL-22a;Il22;Il22a;Iltif;Iltifa |
| 物种 | 小鼠 |
| 表达宿主 | E.coli |
| 序列信息 | Leu34-Val179 |
| 检索号 | Q9JJY9 |
| 分子量 | 16.7 kDa |
| 表观分子量 | 15 kDa |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介绍 | Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue Protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature Protein that shares approximately 79% and 22% sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase Proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 Receptor complex consists of two Receptor subunits, IL-22R (previously an orphan Receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II Cytokine Receptor family. |
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？
如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱
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Q3:细胞因子加无菌水溶解后需要再次离心吗？
加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的
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Q4:细胞因子离心前需要放置室温吗？
不用提前放置室温.
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