OPG/TNFRSF11B (C-6His), Human, Recombinant
英文名称：OPG/TNFRSF11B (C-6His), Human, Recombinant
货号： PCK213
价格： ￥6080 ￥980 ￥320 ￥8680
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 别名 | Tumor Necrosis Factor Receptor Superfamily Member 11B;Osteoclastogenesis Inhibitory Factor;Osteoprotegerin;TNFRSF11B;OCIF;OPG |
| 物种 | 人 |
| 表达宿主 | Human Cells |
| 序列信息 | Glu22-Leu401 |
| 检索号 | O00300 |
| 分子量 | 44.65 kDa |
| 表观分子量 | 57 kDa |
| 生物活性 | Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 10.6 ng/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介绍 | TNFRSF11B is a secreted Protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy Receptor for the Receptor activator of nuclear factor kappa B Ligand (RANKL). By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are Derived from granulocyte/macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK Ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast. |
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？
如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱
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Q3:细胞因子加无菌水溶解后需要再次离心吗？
加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的
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Q4:细胞因子离心前需要放置室温吗？
不用提前放置室温.
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