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| 名称 | NCI-H1299(人非小细胞肺癌细胞) (STR鉴定正确) |
| 别称 | H1299; H-1299; NCIH1299 |
| 背景描述 | NCI-H1299细胞来源于一个淋巴结转移，患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。 |
| 种属 | 人 |
| 年龄（性别） | 男；43岁 |
| 组织来源 | 肺癌：非小细胞肺癌；转移灶：淋巴结 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~30 hours |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 基因表达情况 | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
| 保藏机构 | ATCC; CRL-5803 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 23,24 D3S1358 17 D5S818 11 D7S820 10 D8S1179 10,13 D13S317 12 D16S539 12,13 D18S51 16 D19S433 14 D21S11 32.2 FGA 20 PentaD 13 PentaE 11 TH01 6,9.3 TPOX 8 vWA 16,18 D6S1043 11,13 D12S391 21,22 D2S441 11,13 -
STR鉴定图
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参考文献
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A two-circular RNA signature as a noninvasive diagnostic biomarker for lung adenocarcinoma(2019/02/18)
作者：Xiaoxia Liu, Yie Yang, Xiao Liu
期刊：Journal of Translational Medicine
影响因子 ：8.440
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Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway(2021/03/06)
作者：Xiaoqing Xu, Xinyue Zhang, Yang Zhang
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2021.111439
影响因子 ：7.419
引用产品：164210 164210-50 CL-0005 CL-0016 CL-0165 CL-0166 CL-0249 CL-0299 PB180120 PM150110
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TRIM25 activates AKT/mTOR by inhibiting PTEN via K63-linked polyubiquitination in non-small cell lung cancer(2021/04/30)
作者：Yuanming He, Xiumin Zhou, Shuoyi Jiang
期刊：Acta Pharmacologica Sinica
DOI：10.1038/s41401-021-00662-z
影响因子 ：7.1690
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Ubiquilin 1 suppresses the cancer stem cell–like traits of non-small cell lung cancer cells by regulating reactive oxygen species homeostasis(2021/9/24)
作者：Ting Liu, Qianqian Ma, Wenjie Li
期刊：Bioengineered
DOI：10.1080/21655979.2021.1979353
影响因子 ：6.8320
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Inhibition of miR-185-3p Confers Erlotinib Resistance Through Upregulation of PFKL/MET in Lung Cancers(2021/07/21)
作者：Ke Li, Xinling Zhu, Conghu Yuan
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：6.081
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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma(2021/070/7)
作者：Xiaoxia Dong, Yongyu Liu, Xinzhou Deng
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：6.081
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Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer(2020/04/20)
作者：Fang Luo, Yi Zhao, Jiwei Liu
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2020.105750
影响因子 ：5.652
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Systematic screening reveals synergistic interactions that overcome MAPK inhibitor resistance in cancer cells(2021/6/9)
作者：Yu Yu, Minzhen Tao, Libin Xu
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0560
影响因子 ：5.3470
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者：Chao Yang, Jiang Shi, Jie Wang
期刊：Cell Cycle
DOI：10.1080/15384101.2021.1941612
影响因子 ：5.173
引用产品：164210 164210-50 CL-0165 CL-0496 CL-0519 PB180120 PM150110
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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis(2021/05/04)
作者：Na Li, Dan Zhu
期刊：Cell Cycle
DOI：10.1080/15384101.2021.1919829
影响因子 ：5.173
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LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis(2020/01/13)
作者：Zhanwu Yu
期刊：Cell Cycle
DOI：10.1080/15384101.2019.1708016
影响因子 ：5.173
引用产品：CL-0165
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Ophiopogonin B inhibits migration and invasion in non-small cell lung cancer cells through enhancing the interaction between Axin and β-catenin(2021/08/28)
作者：Shiping Zhang, Hongxiao Li, Liqiu Li
期刊：Journal of Cancer
影响因子 ：4.478
引用产品：CL-0165
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SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway(2020/10/27)
作者：Yan Feng, Xiangyu Guo, Huaping Tang
期刊：Annals of Translational Medicine
影响因子 ：3.9320
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Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis(2020/09/16)
作者：Yurong Jin, Zhenjun Su, Hui Sheng
期刊：Neoplasma
DOI：10.4149/neo_2020_200331N333
影响因子 ：3.4090
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Knockdown of lncRNA MIR503HG suppresses proliferation and promotes apoptosis of non-small cell lung cancer cells by regulating miR-489-3p and miR-625-5p(2020/01/10)
作者：Runa Dao, Muli Wudu, Linping Hui
期刊：Pathology Research and Practice
影响因子 ：3.3090
