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产品概述
名称 | NCI-H1299(人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | H1299; H-1299; NCIH1299 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | RPMI-1640+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
背景描述 | NCI-H1299细胞来源于一个淋巴结转移,患者接受了初期放疗。NCI-H1299细胞均一性的部分缺失p53蛋白,并缺少p53蛋白表达。NCI-H1299细胞可以合成0.1pmol/毫克蛋白的NMB蛋白,而不合成促胃液释放肽(GRP)。 |
年龄(性别) | 男;43岁 |
组织来源 | 肺癌:非小细胞肺癌;转移灶:淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | 1 |
倍增时间 | ~30 hours |
基因表达情况 | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B, The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
保藏机构 | ATCC; CRL-5803 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 23,24 D3S1358 17 D5S818 11 D7S820 10 D8S1179 10,13 D13S317 12 D16S539 12,13 D18S51 16 D19S433 14 D21S11 32.2 FGA 20 PentaD 13 PentaE 11 TH01 6,9.3 TPOX 8 vWA 16,18 D6S1043 11,13 D12S391 21,22 D2S441 11,13 -
STR鉴定图
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参考文献
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Lung cancer shapes commensal bacteria via exosome-like nanoparticles(2022/03/17)
作者:Jipeng Jianga, Jie Mei, Shasha Jiang
期刊:Nano Today
DOI:10.1016/j.nantod.2022.101451
影响因子 :18.9620
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The O-glycosylating enzyme GALNT2 acts as an oncogenic driver in non-small cell lung cancer(2022/09/04)
作者:Qing Hu, Tian Tian, Yahui Leng
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-022-00378-w
影响因子 :8.7020
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A two-circular RNA signature as a noninvasive diagnostic biomarker for lung adenocarcinoma(2019/02/18)
作者:Xiaoxia Liu, Yie Yang, Xiao Liu
期刊:Journal of Translational Medicine
影响因子 :8.440
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Thioredoxin reductase 1 inhibitor shikonin promotes cell necroptosis via SecTRAPs generation and oxygen-coupled redox cycling(2021/12/30)
作者:Yue Zhang, Shibo Sun, Weiping Xu
期刊:Free Radical Biology And Medicine
DOI:10.1016/j.freeradbiomed.2021.12.314
影响因子 :8.1010
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Radiation-induced bystander effect on the genome of bone marrow mesenchymal stem cells in lung cancer(2022/10/14)
作者:Yiming Zhang, Liying Zhang, Yangyang Li
期刊:ANTIOXIDANTS & REDOX SIGNALING
影响因子 :7.4680
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Plumbagin reduction by thioredoxin reductase 1 possesses synergy effects with GLUT1 inhibitor on KEAP1-mutant NSCLC cells(2021/12/23)
作者:Shibo Sun, Yue Zhang, Weiping Xu
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2021.112546
影响因子 :7.4190
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Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway(2021/03/06)
作者:Xiaoqing Xu, Xinyue Zhang, Yang Zhang
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2021.111439
影响因子 :7.419
引用产品:164210 164210-50 CL-0005 CL-0016 CL-0165 CL-0166 CL-0249 CL-0299 PB180120 PM150110
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TRIM25 activates AKT/mTOR by inhibiting PTEN via K63-linked polyubiquitination in non-small cell lung cancer(2021/04/30)
作者:Yuanming He, Xiumin Zhou, Shuoyi Jiang
期刊:Acta Pharmacologica Sinica
DOI:10.1038/s41401-021-00662-z
影响因子 :7.1690
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Ubiquilin 1 suppresses the cancer stem cell–like traits of non-small cell lung cancer cells by regulating reactive oxygen species homeostasis(2021/9/24)
作者:Ting Liu, Qianqian Ma, Wenjie Li
期刊:Bioengineered
DOI:10.1080/21655979.2021.1979353
影响因子 :6.8320
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Hsa_circ_0006677 regulates special AT-rich binding protein-2-mediated tumor-suppressive effect via functioning as a miR-1245a sponge in non-small cell lung cancer(2022/01/26)
作者:Xizhong Sui, Zongzhi Liu, Lei Niu
期刊:Bioengineered
DOI:10.1080/21655979.2022.2031400
影响因子 :6.8320
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer(2022/03/28)
作者:Feiyun Chang , Jiali Li , Quan Sun
期刊:Bioengineered
DOI:10.1080/21655979.2022.2053810
影响因子 :6.8320
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Casticin and chrysosplenol D from Artemisia annua L. induce apoptosis by inhibiting topoisomerase IIα in human non-small-cell lung cancer cells(2022/04/04)
作者:Chunqing Fu, Keyu Zhang, Manyuan Wang
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2022.154095
影响因子 :6.6560
引用产品:CL-0165
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Structurally Diverse Diterpenes from the South China Sea Soft Coral Sarcophyton trocheliophorum(2023/01/20)
作者:Yuting Song, Dandan Yu, Mingzhi Su
期刊:Marine Drugs
影响因子 :6.0850
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Inhibition of miR-185-3p Confers Erlotinib Resistance Through Upregulation of PFKL/MET in Lung Cancers(2021/07/21)
作者:Ke Li, Xinling Zhu, Conghu Yuan
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.081
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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma(2021/070/7)
作者:Xiaoxia Dong, Yongyu Liu, Xinzhou Deng
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.081
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Prognostic Value of Genomic Instability of m 6 A-Related lncRNAs in Lung Adenocarcinoma(2022/03/03)
作者:Rui Li, JianPing Li, TingTing Liu
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.0810
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Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3(2022/11/12)
作者:Wu Xu, Zhang Hui, Jiang Gang, Peng Minlian, Li Cheng, Lu Jiaxin, Jiang Shiyin, Yang Xiaoping, Jiang Yongliang
期刊:CLINICAL AND EXPERIMENTAL IMMUNOLOGY
影响因子 :5.7320
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Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer(2020/04/20)
作者:Fang Luo, Yi Zhao, Jiwei Liu
期刊:International Journal Of Biochemistry & Cell Biology
DOI:10.1016/j.biocel.2020.105750
影响因子 :5.652
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer(2022/11/27)
作者:Tan, Bo, Zhang, Jingwei, Wang, Wen, Ma, Haibo, Yang, Yuanyuan
期刊:APOPTOSIS
DOI:10.1007/s10495-022-01789-y
影响因子 :5.5610
引用产品:CL-0016 CL-0165 CL-0298 CL-0496 CL-0668 CM-0496 PCK001 PM150110
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Systematic screening reveals synergistic interactions that overcome MAPK inhibitor resistance in cancer cells(2021/6/9)
作者:Yu Yu, Minzhen Tao, Libin Xu
期刊:Cancer Biology & Medicine
DOI:10.20892/j.issn.2095-3941.2020.0560
影响因子 :5.3470
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Light and hydrogen peroxide dual-responsive DNA interstrand crosslink precursors with potent cytotoxicity(2022/11/12)
作者:Yuanyuan Jia, Jing Sun, Dehao Yu, Luo Wang, Anahit Campbell, Heli Fan, Huabing Sun
期刊:BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2022.106270
影响因子 :5.3070
引用产品:CL-0165
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者:Chao Yang, Jiang Shi, Jie Wang
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1941612
影响因子 :5.173
引用产品:164210 164210-50 CL-0165 CL-0496 CL-0519 PB180120 PM150110
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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis(2021/05/04)
作者:Na Li, Dan Zhu
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1919829
影响因子 :5.173
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LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis(2020/01/13)
作者:Zhanwu Yu
期刊:Cell Cycle
DOI:10.1080/15384101.2019.1708016
影响因子 :5.173
引用产品:CL-0165
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Black phosphorus nanoparticles for dual therapy of non-small cell lung cancer(2022/02/02)
作者:Zhongxiao Lin, Qiudi Deng, Qi Fang
期刊:Journal Of Drug Targeting
DOI:10.1080/1061186X.2022.2032093
影响因子 :5.0160
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Ophiopogonin B inhibits migration and invasion in non-small cell lung cancer cells through enhancing the interaction between Axin and β-catenin(2021/08/28)
作者:Shiping Zhang, Hongxiao Li, Liqiu Li
期刊:Journal of Cancer
影响因子 :4.478
引用产品:CL-0165
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Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1(2022/02/17)
作者:Hongyang Shi, Yonghong Zhang, Yu Wang
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2022.115926
影响因子 :4.4600
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LINC00961 functions as an anti-oncogene in non-small cell lung carcinoma by regulation of miR-3127(2022/02/15)
作者:YongGang Liu, Jia Li, Fang Nie
期刊:American Journal of Translational Research
DOI:PMID:35273692
影响因子 :3.9400
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SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway(2020/10/27)
作者:Yan Feng, Xiangyu Guo, Huaping Tang
期刊:Annals of Translational Medicine
影响因子 :3.9320
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Circ_0025039 acts an oncogenic role in the progression of non-small cell lung cancer through miR-636-dependent regulation of CORO1C(2022/01/16)
作者:Lei Wang, Cimei Zeng, Zhongren Chen
期刊:Molecular and Cellular Biochemistry
DOI:10.1007/s11010-021-04320-0
影响因子 :3.8420
引用产品:164210 164210-50 CL-0016 CL-0122 CL-0165 CL-0249 PB180120 PM150110 PM150910
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Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis(2020/09/16)
作者:Yurong Jin, Zhenjun Su, Hui Sheng
期刊:Neoplasma
DOI:10.4149/neo_2020_200331N333
影响因子 :3.4090
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Knockdown of lncRNA MIR503HG suppresses proliferation and promotes apoptosis of non-small cell lung cancer cells by regulating miR-489-3p and miR-625-5p(2020/01/10)
作者:Runa Dao, Muli Wudu, Linping Hui
期刊:Pathology Research and Practice
影响因子 :3.3090
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IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation(2022/10/30)
作者:Wenping Liu, Miaomiao Xin, Qing Li
期刊:Biomed Research International
影响因子 :3.2460
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Circ_0002476 regulates cell growth, invasion, and mtDNA damage in non–small cell lung cancer by targeting miR-1182/TFAM axis(2022/09/03)
作者:Weijie Wang, Haiting Sun, Xuan Ma
期刊:Thoracic Cancer
影响因子 :3.2230
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma(2023/02/24)
作者:Rui Chen, Zhiping Wang, Tianzhu Lu
期刊:Thoracic Cancer
影响因子 :3.2230
引用产品:CL-0016 CL-0161 CL-0165 CL-0166 CL-0298 CL-0668 CM-0161
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Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis(2021/11/19)
作者:Rui Zhang, Liang Shang, Jinniang Nan
期刊:Autoimmunity
DOI:10.1080/08916934.2021.2001801
影响因子 :2.9570
引用产品:164210 164210-50 CL-0016 CL-0165 CL-0166 CL-0229 PB180120 PM150110
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Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway(2022/10/26)
作者:Wenqian Hu, Hongjiao Wu, Ang Li
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7510
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Anti-tumor cembrane diterpenoids from the South China Sea soft coral Lobophytum sp.(2023/02/28)
作者:Fan Yang, Yuting Song, Dandan Yu
期刊:CHEMISTRY & BIODIVERSITY
影响因子 :2.7450
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Ginsenoside Rg3 enhances the radiosensitivity of lung cancer A549 and H1299 cells via the PI3K/AKT signaling pathway(2023/02/15)
作者:Jialin Li, Bo Yang
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-023-00749-3
影响因子 :2.7230
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Identification of a Novel circ_0010235/miR-1249-3p/HOXA13 Axis in Lung Adenocarcinoma(2022/01/25)
作者:Jiancheng Liu, Zejun Fu
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10181-5
影响因子 :2.2200
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Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage(2021/11/01)
作者:Haohao Zhu, Huai Liu, Jianfeng Wen
期刊:Applied Immunohistochemistry & Molecular Morphology
DOI:10.1097/PAI.0000000000000963
影响因子 :1.9920
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miR-137 represses migration and cell motility by targeting COX-2 in non-small cell lung cancer(2022/10/01)
作者:Yutu Luo, Suwei Hu, Fang Wang
期刊:Translational Cancer Research
影响因子 :0.4960