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产品概述
| 名称 | NCI-N87 [N87] (人胃癌细胞) (STR鉴定正确) |
| 别称 | NCI-N87 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | NCI-N87细胞表达表面糖蛋白癌胚抗原(CEA)和TAG72，并且没有左旋多巴胺脱羧酶(DDC)活性。NCI-N87细胞的血管活性的肠肽(VIP)受体活性极低并缺乏胃泌激素受体，它们表达蕈毒碱胆碱受体。没有证据表明，NCI-N87细胞存在N-myc、L-myc、myb和EGF受体基因的重组。NCI-N87细胞表达的c-myc和c-erb-B2 RNA水平与其它细胞株相当。以下基因NCI-N87细胞不表达：N-myc、L-myc、c-cis、IGF-2或胃泌激素释放肽。据报道，NCI-N87细胞的植板率为4.3%。 |
| 年龄（性别） | 男 |
| 组织来源 | 胃癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 胃癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~48-80 hours |
| 致瘤性 | Yes, the cells form tumors in athymic nude mice. |
| 受体表达情况 | acetylcholine, muscarinic, expressed |
| 保藏机构 | ATCC; CRL-5822 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 8,12 D2S1338 23,24 D3S1358 14 D5S818 12,13 D7S820 10,11 D8S1179 14 D13S317 8,11 D16S539 9,13 D18S51 17 D19S433 14,14.2 D21S11 30 FGA 20,21 PentaD 12 PentaE 5 TH01 9 TPOX 9,11 vWA 15,16 D6S1043 12 D12S391 16,21 D2S441 11,12 -
STR鉴定图
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参考文献
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RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer(2019/10/21)
作者：Yang Yu, Lei Chen, Guiping Zhao
期刊：Oncogene
影响因子 ：8.7560
引用产品：CL-0169
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Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment(2021/07/16)
作者：Chenghao Li, Jiawei Li, Yan Li
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：5.895
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Circ_0021087 acts as a miR-184 sponge and represses gastric cancer progression by adsorbing miR-184 and elevating FOSB expression(2021/06/02)
作者：Yin Yu, Hong Li, Chunhua Wu
期刊：European Journal Of Clinical Investigation
影响因子 ：5.722
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Silencing of Nudix type 5 represses proliferation and invasion and enhances chemosensitivity of gastric carcinoma cells by affecting the AKT/GSK-3β/β-catenin pathway(2022/03/02)
作者：Dong Tan, Yafei Zhang
期刊：Toxicology And Applied Pharmacology
DOI：10.1016/j.taap.2022.115968
影响因子 ：4.4600
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Pyrotinib treatment on HER2-positive gastric cancer cells promotes the released exosomes to enhance endothelial cell progression, which can be counteracted by apatinib(2019/3/12)
作者：Zhengxing Gao, Chunqing Song, Guangxin Li
期刊：Onco Targets and Therapy
影响因子 ：4.3450
引用产品：CL-0169
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Demethylase FTO enhances the PI3K/Akt signaling to promote gastric cancer malignancy(2023/03/27)
作者：Yuxuan Zhu, Jing Yang, Yongxiang Li
期刊：MEDICAL ONCOLOGY
DOI：10.1007/s12032-023-01990-2
影响因子 ：3.7380
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Circ_0032821 Facilitates Gastric Cancer Cell Proliferation, Migration, Invasion and Glycolysis by Regulating MiR-1236-3p/HMGB1 Axis(2020/10/12)
作者：Lei Chen, Kun Chi, Huaguo Xiang
期刊：Cancer Management and Research
影响因子 ：3.6020
引用产品：164210 164210-50 CL-0022 CL-0107 CL-0169 CL-0292 CL-0563 PB180120 PM150110
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Knockdown of Serum- and Glucocorticoid-Regulated Kinase 1 Enhances Cisplatin Sensitivity of Gastric Cancer Through Suppressing the Nuclear Factor Kappa-B Signaling Pathway.(2021/11/01)
作者：Jishui Zhang, Wenhao Lv, Yagang Liu
期刊：Balkan Medical Journal
DOI：10.5152/balkanmedj.2021.21114
影响因子 ：3.5700
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ZNF521/EBF1 axis regulates AKR1B1 to promote the proliferation, migration, and invasion of gastric cancer cells(2022/11/17)
作者：Yu Cheng, Yi-Jiang Ni, Li-Ming Tang
期刊：KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子 ：2.9960
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Nucleoporin 37 promotes the cell proliferation, migration, and invasion of gastric cancer through activating the PI3K/AKT/mTOR signaling pathway(2021/12/09)
作者：Jishui Zhang, Wenhao Lv, Yagang Liu
期刊：In Vitro Cellular & Developmental Biology - Animal
DOI：10.1007/s11626-021-00627-w
影响因子 ：2.7230
引用产品：CL-0169
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Ubiquitin-specific protease 1 overexpression indicates poor prognosis and promotes proliferation, migration, and invasion of gastric cancer cells(2021/12/29)
作者：Debin Meng, Delong Li
期刊：Tissue & Cell
DOI：10.1016/j.tice.2021.101723
影响因子 ：2.5860
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Knockdown of kinesin family member 2C restricts cell proliferation and induces cell cycle arrest in gastric cancer(2022/11/30)
作者：Shuai Li, Yulian Ma, Chen Wu, Xinfang Hou, Shuai Li, Yulian Ma, Chen Wu, Xinfang Hou
期刊：HISTOLOGY AND HISTOPATHOLOGY
影响因子 ：2.1300
