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产品概述
| 名称 | NIH/3T3 (小鼠胚胎细胞) (STR鉴定正确) |
| 别称 | NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | NIH/3T3细胞是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。NIH/3T3细胞对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用；NIH/3T3细胞鼠痘病毒阴性。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胚胎 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~20 hours |
| 保藏机构 | ATCC; CRL-1658 ATCC; CRL-6442DSMZ; ACC-59 ECACC; 93061524 |
STR鉴定
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STR位点信息
18-3 17,19 6-7 12 5-5 14,15 X-1 25 1-2 13,17 7-1 29 8-1 15 1-1 10 19-2 11,12 15-3 20.3 12-1 20 6-4 16 4-2 19.3,20.3 3-2 14,15 2-1 9 13-1 16.2
11-2 15,17 17-2 13,14 -
STR鉴定图
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参考文献
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Triple Enzyme-Regulated Molecular Hydrogels for Carrier-Free Delivery of Lonidamine(2021/07/29)
作者：Can Wu, Chunlu Wang, Yaoyao Zheng
期刊：Advanced Functional Materials
影响因子 ：19.000
引用产品：CL-0171
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Triple Enzyme-Regulated Molecular Hydrogels for Carrier-Free Delivery of Lonidamine(2021/07/29)
作者：Can Wu, Chunlu Wang, Yaoyao Zheng
期刊：Advanced Functional Materials
影响因子 ：19.000
引用产品：CL-0171
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Ultrasound-triggered piezocatalytic composite hydrogels for promoting bacterial-infected wound healing(2022/12/14)
作者：Dun Liu, Lei Li, Benlong Shi
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2022.11.023
影响因子 ：18.900
引用产品：CL-0171
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A self-monitoring microneedle patch for light-controlled synergistic treatment of melanoma(2023/03/27)
作者：Chenyuan Wang, Yongnian Zeng, Kaifeng Chen
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2023.03.016
影响因子 ：18.900
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Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation(2023/10/13)
作者：Jiayu Liu, Chuanrong Zhao, Xue Xiao
期刊：Nature Communications
DOI：10.1038/s41467-023-42341-z
影响因子 ：16.600
引用产品：CL-0171
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The miR-669a-5p/G3BP/HDAC6/AKAP12 Axis Regulates Primary Cilia Length(2023/12/13)
作者：Weina Wang, Xuyao Dai, Yue Li
期刊：Advanced Science
影响因子 ：15.100
引用产品：CL-0171
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Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells(2022/08/12)
作者：Qi Wang, Feng Xiao, Haomiao Su
期刊：NUCLEIC ACIDS RESEARCH
影响因子 ：14.900
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MOFs-Based Nanoagents Enable Sequential Damage to Cancer-Associated Fibroblast and Tumor Cells for Phototriggered Tumor Microenvironment Regulation(2023/08/31)
作者：Jiaqi Meng, Weier Bao, Ming Liu
期刊：Small
影响因子 ：13.300
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Biocompatible and biodegradable chitin-based hydrogels crosslinked by BDDE with excellent mechanical properties for effective prevention of postoperative peritoneal adhesion(2023/01/05)
作者：Yongkang Zou, Pengpeng Yue, Hankun Cao
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.120543
影响因子 ：11.200
引用产品：CL-0171
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Injectable chitosan/xyloglucan composite hydrogel with mechanical adaptivity and endogenous bioactivity for skin repair(2023/04/13)
作者：Hua Wei, Shengkai Yu, Yansheng Zhang
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.120904
影响因子 ：11.200
引用产品：CL-0171
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Nanoparticle-Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs(2022/03/04)
作者：Jie Tao, Shunyao Zhu, Nazi Zhou
期刊：Advanced Healthcare Materials
影响因子 ：11.092
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Eco-friendly and degradable red phosphorus nanoparticles for rapid microbial sterilization under visible light(2020/07/26)
作者：Qian Wu, Xiangmei Liu, Bo Li
期刊：Journal of Materials Science & Technology
DOI：10.1016/j.jmst.2020.04.084
影响因子 ：10.900
引用产品：CL-0171
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Scaffold-free Tracheal Engineering via a Modular Strategy Based on Cartilage and Epithelium Sheets(2022/12/02)
作者：Minglei Yang, Jiafei Chen, Yi Chen
期刊：Advanced Healthcare Materials
影响因子 ：10.000
引用产品：CL-0171
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Multifunctional MOF-Based Microneedle Patch With Synergistic Chemo-Photodynamic Antibacterial Effect and Sustained Release of Growth Factor for Chronic Wound Healing(2023/03/20)
作者：Yongnian Zeng, Chenyuan Wang, Kai Lei
期刊：Advanced Healthcare Materials
影响因子 ：10.000
引用产品：CL-0171
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Enzyme-Responsive Modular Peptides Enhance Tumor Penetration of Quantum Dots via Charge Reversal Strategy(2024/01/27)
作者：Qiuju Dai, Zhen Du, Lihong Jing
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.500
引用产品：CL-0171
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MMP14high macrophages orchestrate progressive pulmonary fibrosis in SR-Ag-induced hypersensitivity pneumonitis(2024/01/11)
作者：Dan Peng, Juan Li, Yin Li
期刊：PHARMACOLOGICAL RESEARCH
DOI：10.1016/j.phrs.2024.107070
影响因子 ：9.300
引用产品：CL-0171
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Genetically engineered bi-functional silk material with improved cell proliferation and anti-inflammatory activity for medical application(2018/12/23)
作者：Yuancheng Wang, Feng Wang, Sheng Xu
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.12.036
影响因子 ：9.300
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Gelatin-based dynamic response antioxidant, anti-inflammatory multifunctional hydrogel for enhanced diabetic wound repair(2024/01/20)
作者：Dong Yang, Zeyu Shou, Xiaoling Xie
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.129453
影响因子 ：8.200
引用产品：CL-0171
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Doping proanthocyanidins into gel/zirconium hybrid hydrogel to reshape the microenvironment of diabetic wounds for healing acceleration(2024/01/17)
作者：Yaxin Zhu, Dong Yang, Jieyu Liu
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.129353
影响因子 ：8.200
引用产品：CL-0171
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Enhanced single RNA imaging reveals dynamic gene expression in live animals(2023/03/03)
作者：Yucen Hu, Jingxiu Xu, Erqing Gao
期刊：eLife
影响因子 ：7.700
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MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression(2022/01/12)
作者：Jinti Lin, Ankai Xu, Jiakang Jin
期刊：OncoImmunology
DOI：10.1080/2162402X.2021.2024941
影响因子 ：7.200
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The miR-21-5p enriched in the apoptotic bodies of M2 macrophage-derived extracellular vesicles alleviates osteoarthritis by inducing a change in the macrophage phenotype(2022/10/05)
作者：Leilei Qin, Jianye Yang, Xudong Su
期刊：Genes & Diseases
DOI：10.1016/j.gendis.2022.09.010
影响因子 ：6.800
引用产品：CL-0171
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A confined crosslinking strategy towards an intelligent organosilica–micellar hybrid drug delivery system(2022/1/18)
作者：Xing Qin, Limei Qin, Jianping He
期刊：Biomaterials Science
影响因子 ：6.600
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Stromal and tumor immune microenvironment reprogramming through multifunctional cisplatin-based liposomes boosts the efficacy of anti-PD-1 immunotherapy in pancreatic cancer(2023/11/03)
作者：Hang Yu, Wenting Zhu, Caiyan Lin
期刊：Biomaterials Science
影响因子 ：6.600
引用产品：CL-0171
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Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing(2022/08/17)
作者：Hongli Zhang, Xu Zhang, Qi Cao
期刊：Biomaterials Science
影响因子 ：6.600
引用产品：CL-0171
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Effect of manganese ion doping on magnetic properties and magnetic induction heating of Zn-Al ferrite nanoparticles(2023/07/31)
作者：Yifan Zheng, Renpeng Yang, Xiaogang Yu
期刊：JOURNAL OF ALLOYS AND COMPOUNDS
DOI：10.1016/j.jallcom.2023.171597
影响因子 ：6.200
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Structural, physicochemical properties and function of swim bladder collagen in promoting fibroblasts viability and collagen synthesis(2022/12/09)
作者：Lanfei Xiao, Jiehuan Lv, Yongjun Liang
期刊：LWT-FOOD SCIENCE AND TECHNOLOGY
影响因子 ：6.000
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Sawdust-derived cellulose nanofibrils with high biosafety for potential bioprinting(2024/01/09)
作者：Haixin Jiao, Yifei Shi, Jianzhong Sun
期刊：INDUSTRIAL CROPS AND PRODUCTS
DOI：10.1016/j.indcrop.2024.118025
影响因子 ：5.900
引用产品：CL-0171
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Nano-baicalein facilitates chemotherapy in breast cancer by targeting tumor microenvironment(2023/02/24)
作者：Fang Zheng, Yujia Luo, Yuanqi Liu
期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2023.122778
影响因子 ：5.800
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Multi-targeting liposomal codelivery of cisplatin and rapamycin inhibits pancreatic cancer growth and metastasis through stromal modulation(2023/08/14)
作者：Wenting Zhu, Hang Yu, Menglei Jia
期刊：INTERNATIONAL JOURNAL OF PHARMACEUTICS
DOI：10.1016/j.ijpharm.2023.123316
影响因子 ：5.800
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Triggering Drug Release and Thermal-Disrupting Interface Induced Mitigation of Composite Photothermal Hydrogel Treating Infectious Wounds( 2021/12/13)
作者：Long Hua, Hu Qian, Ting Lei
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：5.700
引用产品：CL-0171
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Mussel-inspired biocompatible polydopamine/carboxymethyl cellulose/polyacrylic acid adhesive hydrogels with UV-shielding capacity(2021/01/02)
作者：Zuwu Tang, Yanan Miao, Jing Zhao
期刊：Cellulose
DOI：10.1007/s10570-020-03596-7
影响因子 ：5.700
引用产品：CL-0171
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Peficitinib ameliorates doxorubicin-induced cardiotoxicity by suppressing cellular senescence and enhances its antitumor activity(2023/07/12)
作者：Hui Hua, Qi Zhao, Jing Xia
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110630
影响因子 ：5.600
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Tetrandrine Alleviates Silica-induced Pulmonary Fibrosis Through PI3K/AKT Pathway: Network Pharmacology Investigation and Experimental Validation(2024/01/24)
作者：Ruimin Ma, Xiaoxi Huang, Di Sun
期刊：INFLAMMATION
DOI：10.1007/s10753-023-01964-6
影响因子 ：5.100
引用产品：CL-0171
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Allyl phenyl selenides as H2O2 acceptors to develop ROS-responsive theranostic prodrugs(2022/09/17)
作者：Xueyan Yang, Bo Yuan, Hai Xiong
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2022.106154
影响因子 ：5.100
引用产品：CL-0171
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Danshensu alleviates bleomycin-induced pulmonary fibrosis by inhibiting lung fibroblast-to-myofibroblast transition via the MEK/ERK signaling pathway(2021/6/30)
作者：Huaman Liu, Xinyue Zhang, Yumeng Shao
期刊：Bioengineered
DOI：10.1080/21655979.2021.1944020
影响因子 ：4.900
引用产品：CL-0171
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Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds(2022/12/11)
作者：Yuwei Li, Yu Liu, Senio Campos de Souza
期刊：Journal of Functional Biomaterials
影响因子 ：4.800
引用产品：CL-0171
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Two-Pore-Domain Potassium Channel TREK–1 Mediates Pulmonary Fibrosis through Macrophage M2 Polarization and by Direct Promotion of Fibroblast Differentiation(2023/04/26)
作者：Yunna Zhang, Jiafeng Fu, Yang Han
期刊：Biomedicines
DOI：10.3390/biomedicines11051279
影响因子 ：4.700
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Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers(2022/10/20)
作者：Hui Liu, Xiao Liu, Yuanqiao Cao
期刊：CHINESE JOURNAL OF POLYMER SCIENCE
影响因子 ：4.300
引用产品：CL-0171
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microRNA‐542‐5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen‐activated protein kinase signaling pathway through the negative regulation of P21‐activated kinase 1(2018/9/14)
作者：Xingmao Wu, Kaiqiang Ji, Haiyuan Wang
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.000
引用产品：CL-0171
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Imperatorin derivative OW1, a new vasoactive compound, attenuates cell proliferation and migration by inhibiting Nox1-mediated oxidative stress(2023/02/15)
作者：Nan Zhou, Suyun Yong, Xianpeng Shi
期刊：JOURNAL OF PHARMACY AND PHARMACOLOGY
影响因子 ：3.300
引用产品：CL-0171
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Adjusting the accuracy of PEGDA-GelMA vascular network by dark pigments via digital light processing printing:(2021/11/05)
作者：Lin Sheng, Mo Li, Shuxian Zheng
期刊：Journal Of Biomaterials Applications
影响因子 ：2.900
引用产品：CL-0171
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Study on properties and biocompatibility of poly (butylene succinate) and sodium alginate biodegradable composites for biomedical applications(2022/08/26)
作者：Shanshan Wang, Quansheng Xing
期刊：Materials Research Express
影响因子 ：2.300
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Long Non-Coding RNA Dancr Affects Myocardial Fibrosis in Atrial Fibrillation Mice via the MicroRNA-146b-5p/Smad5 Axis(2023/11/01)
作者：Dejin Wang, Xiqian Wang, Tianxiao Yang
期刊：Acta Cardiologica Sinica
DOI：10.6515/ACS.202311_39(6).20230619B
影响因子 ：1.900
引用产品：CL-0171
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Antibacterial HPL-DOSS surface coating for commercial plastics(2023/09/11)
作者：Guohao Chen, Xiao Liu, Rui Zhang
期刊：JOURNAL OF THE CHINESE CHEMICAL SOCIETY
影响因子 ：1.800
引用产品：CL-0171
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Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation(2017/08/07)
作者：Fengming Zhu, Octavia L S Chong Lee Shin, Guangchang Pei
期刊：Oncotarget
影响因子 ：0.000
引用产品：CL-0171
