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产品概述
名称 | RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确) |
别称 | RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7 |
种属 | 小鼠 |
年龄(性别) | 雄;成年 |
组织来源 | Abelson鼠科白血病病毒诱导的肿瘤;单核细胞;巨噬细胞 |
生长特性 | 贴壁细胞 |
细胞形态 | 不规则形 |
背景描述 | RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤;sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖,并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球,但对肿瘤靶细胞无作用。 |
生物安全等级 | 2 |
生长培养基 | DMEM(PM150210)+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~12-30 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | complement (C3) |
抗原表达情况 | H-2d |
基因表达情况 | lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox). |
保藏机构 | ATCC; TIB-71 ECACC; 91062702 |
STR鉴定
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STR位点信息
18-3 18 9-2 14.3 6-7 12 5-5 14 X-1 23.3 15-3 22.3 12-1 15.3 6-4 17.3 4-2 20.3 jaridl X,Y -
STR鉴定图
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参考文献
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Evaluation of efficacy on RANKL induced osteoclast from RAW264.7 cells(2018.12.04)
作者:Chengchao Song, Xiaobin Yang, Yongsheng Lei, Zhen Zhang, Wanli Smith, Jinglong Yan
期刊:journal of cellular physiology
影响因子 :5.546
引用产品:CL-0190
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Cellular study of the LPS‐induced osteoclastic multinucleated cell formation from RAW264.7 cells(2019.06.21)
作者:Yuan Xiao, Yang Cao, Chengchao Song
期刊:Journal of Cellular Physiology
影响因子 :5.546
引用产品:CL-0190
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Activation of RAW264.7 macrophages by an acidic polysaccharide derived from Citrus grandis ‘Tomentosa’(2020.08.01)
作者:Ruiyi Fan, Congyi Zhu, Diyang Qiu
期刊:International Journal of Biological Macromolecules
DOI:10.1016/j.ijbiomac.2019.11.172
影响因子 :5.162
引用产品:CL-0190
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Dual-functional lipid polymeric hybrid pH-responsive nanoparticles decorated with cell penetrating peptide and folate for therapy against rheumatoid arthritis(19 June 2018)
作者:Jinlong Zhaoa, Xueyan Zhanga, Xiangshi Sun
期刊:European Journal of Pharmaceutics and Biopharmaceutics
DOI:10.1016/j.ejpb.2018.06.020
影响因子 :4.604
引用产品:CL-0190
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Study of double-targeting nanoparticles loaded with MCL-1 siRNA and dexamethasone for adjuvant-induced arthritis therapy(September 2020)
作者:Xiangyu Li, Changhui Yu, Xiangxue Meng
期刊:European Journal of Pharmaceutics and Biopharmaceutics
DOI:10.1016/j.ejpb.2020.07.009
影响因子 :4.604
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Dual-functional lipid polymeric hybrid pH-responsive nanoparticles decorated with cell penetrating peptide and folate for therapy against rheumatoid arthritis(2018.09)
作者:Jinlong Zhao, Xueyan Zhang, Xiangshi Sun
期刊:European Journal of Pharmaceutics and Biopharmaceutics
DOI:10.1016/j.ejpb.2018.06.020
影响因子 :4.604
引用产品:CL-0190
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Three new monoterpene glycosides from oil peony seed cake(2018.01)
作者:PuLiuYu Zhang, Yi-Fan Xu, Xin-Yue Zhu
期刊:Industrial Crops and Products
DOI:10.1016/j.indcrop.2017.10.043
影响因子 :4.244
引用产品:CL-0190
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Tantalum boride as a biocompatible coating to improve osteogenesis of the bionano interface(2020.03.29)
作者:Ruiyan Li, Guancong Liu, Lina Yang, Yun'an Qing
期刊:J Biomed Mater Res A
影响因子 :3.525
引用产品:CL-0190
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Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway(2019.07.16)
作者:Yan Jiang
期刊:Journal of Drug Targeting
DOI:10.1080/1061186X.2019.1616296
影响因子 :3.38
引用产品:CL-0190
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Curcumin prevents osteocyte apoptosis by inhibiting M1‐type macrophage polarization in mice model of glucocorticoid‐associated osteonecrosis of the femoral head(2020.02.03)
作者:Shengyang Jin, Chunqing Meng, Yu He
期刊:Journal of Orthopaedic Research
影响因子 :2.728
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Electrochemical sensing of H2O2 released from living cells based on AuPd alloy-modified PDA nanotubes(2019.02.20)
作者:Guangli He, Fengli Gao, Wei Li, Pengwei Li
期刊:Analytical Methods
影响因子 :2.596
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Placenta‑derived mesenchymal stem cells ameliorate lipopolysaccharide‑induced inflammation in RAW264.7 cells and acute lung injury in rats(2020.06.15)
作者:Wen Yuan Heng‑Ya Song Jie Xiong
期刊:Molecular Medicine Reports
影响因子 :2.1
引用产品:CL-0190
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Artemargyinolide E, a new sesquiterpene lactone from Artemisia argyi inhibits inflammatory responses via down-regulating NF-κB signaling pathway(2020.04)
作者:Lai-Bin Zhang, Hui-Hui Zhu
期刊:Phytochemistry Letters
DOI:10.1016/j.phytol.2020.01.009
影响因子 :1.459
引用产品:CL-0190
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Anti-inflammatory activities of gentiopicroside against iNOS and COX-2 targets(2019.01)
作者:Qi-li Zhang, Jian Zhang, Peng-fei Xia
期刊:Chinese Herbal Medicines
DOI:10.1016/j.chmed.2018.10.004
影响因子 :0
引用产品:CL-0190