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  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)

RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)

货号:CL-0190

规格:1×10⁶cells/T25培养瓶

价格: ¥1600

生长培养基:DMEM10% FBS1% P/S

数量: - +

  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
  • RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)

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RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确) ¥1600
RAW264.7细胞专用培养基
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产品概述
产品信息
名称 RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确)
别称 RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
背景描述 RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤;sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖,并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球,但对肿瘤靶细胞无作用。
种属 小鼠
年龄(性别) 雄性,成年
组织来源 Abelson鼠科白血病病毒诱导的肿瘤;单核细胞;巨噬细胞
生长特性 贴壁细胞,不用胰酶消化
细胞形态 不规则形
细胞类型 肿瘤细胞
肿瘤类型 白血病细胞
生物安全等级 2
生长培养基 DMEM10% FBS1% P/S
推荐传代比例 1:6~1:8
推荐换液频率 2~3次/周
倍增时间 ~12-30小时
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养条件 气相:空气,95%;CO2,5% 温度:37℃
受体表达情况 complement (C3)
抗原表达情况 H-2d
基因表达情况 lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox).
注意事项 1、该细胞不建议使用胰酶消化,胰酶会刺激分化; 2、 超过3天不传代细胞容易分化; 3、 培养时,存在少量分化细胞,属于正常现象。
保藏机构 ATCC; TIB-71 ECACC; 91062702
STR鉴定
  • STR位点信息
    18-3 18
    6-7 12
    5-5 14
    X-1 24
    1-2 17
    7-1 25.2
    8-1 13
    1-1 15,16
    19-2 14
    15-3 22.3
    12-1 16
    6-4 18
    4-2 22.3
    3-2 14
    2-1 16
    13-1 16.2
    11-2 17
    17-2 14,16
  • STR鉴定图
参考文献
文献列表
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07普诺赛技术支持-徐婷

【 北京 】

08普诺赛技术支持-赵家麒

08普诺赛技术支持-赵家麒

【 湖南、天津、河北、山西、内蒙古 】

09普诺赛技术支持-黄玲

09普诺赛技术支持-黄玲

【 四川、陕西、甘肃、重庆、西藏、浙江 】

02普诺赛技术支持-张洁

02普诺赛技术支持-张洁

【 湖北、江西、新疆、海南 】

03普诺赛技术支持-林梦君

03普诺赛技术支持-林梦君

【 上海、宁夏、青海、山东、福建 】

04普诺赛技术支持-李欢欢

04普诺赛技术支持-李欢欢

【 广东 、广西、贵州、云南 】

05普诺赛技术支持-黄鹂

05普诺赛技术支持-黄鹂

【 辽宁、黑龙江、吉林、河南 】