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![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-1.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-2.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-3.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-4.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-5.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0211-6.jpg)
![SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | SK-BR-3 [SKBR3] (人乳腺腺癌细胞) (STR鉴定正确) |
| 别称 | SK-Br-3; Sk-Br-3; SK BR 03; SKBR-3; SKBr-3; SK-BR3; SKBr3; SkBr3; SKBR3 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1. SK-BR-3细胞从冷冻保存中恢复的速度可能很慢。SK-BR-3细胞在低温保存恢复过程中的附着会很轻。这很正常。这种细胞系在培养的整个第一周以及每次继代培养后都表现出松散的附着和漂浮的细胞，这并不少见；2. 如果存在大量漂浮物，尤其是在生长的第一周，在启动复苏后几天内保持细胞不受干扰。如果仍然有很多漂浮物，用台盼蓝检查生存能力。通过轻轻离心保留漂浮细胞，并在更换培养基时将其与附着的细胞一起添加回同一培养容器中，这一点很重要。不要分离或丢弃漂浮细胞。细胞最初以小斑块或细胞群的形式附着，许多细胞团仍处于悬浮状态。几天后，生长将从贴壁细胞群向外延伸。通常也会出现一些细胞碎片。漂浮细胞应始终通过温和离心（125xg，持续5-7min）保留，并在换液或传代后放回培养容器；3. SK-BR-3细胞倾向于相互堆积。在细胞达到70-80%汇合度之前不要用胰酶消化处理细胞。如果让细胞过度生长，细胞就会分离漂浮。 |
| 背景描述 | SK-BR-3 [SKBR3]细胞是由Trempe·G和Old·L·J于1970年从一位43岁的白人女性乳腺癌患者的胸腔积液中分离得到的。SK-BR-3 [SKBR3]细胞亚显微结构特征包括微丝和桥粒、肝糖原颗粒、大溶酶体、成束的细胞质纤丝；SK-BR-3 [SKBR3]细胞过表达HER2/c-erb-2基因产物。 |
| 年龄（性别） | 女性；43岁 |
| 组织来源 | 乳腺；乳房；肋膜渗出液转移灶 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 乳腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~48-72 hours |
| 致瘤性 | Yes, in nude mice; forms poorly differentiated adenocarcinoma. |
| 受体表达情况 | Blood Type A; Rh+; HLA A11, Bw22 (+/-), B40, B18 |
| 保藏机构 | ATCC; HTB-30 DSMZ; ACC-736 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 20,25 D3S1358 17 D5S818 9,12 D7S820 9,12 D8S1179 12 D13S317 11,12 D16S539 9 D18S51 10,13 D19S433 14 D21S11 30,30.2 FGA 20 PentaD 9,12 PentaE 10,11 TH01 8,9 TPOX 8,11 vWA 17 D6S1043 11,19 D12S391 18,20 D2S441 13,14 -
STR鉴定图
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参考文献
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Highly sensitive surface-enhanced Raman scattering (SERS) imaging for phenotypic diagnosis and therapeutic evaluation of breast cancer(2023/01/18)
作者：Chonggui Qiu, Wei Zhang, Yanhong Zhou
期刊：CHEMICAL ENGINEERING JOURNAL
影响因子 ：15.100
-
Profiling of single-vesicle surface proteins via droplet digital immuno-PCR for multi-subpopulation extracellular vesicles counting towards cancer diagnostics(2023/06/24)
作者：Chunchen Liu, Huixian Lin, Jingyun Guo
期刊：CHEMICAL ENGINEERING JOURNAL
影响因子 ：15.100
-
Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging(2023/08/04)
作者：Yuanyuan Zhu, Liang Song, Yongqu Zhang
期刊：CANCER RESEARCH
DOI：10.1158/0008-5472.CAN-22-3432
影响因子 ：11.200
引用产品：CL-0007 CL-0040 CL-0041 CL-0149 CL-0211 CL-0228 CL-0525
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PRMT5 reduces immunotherapy efficacy in triple-negative breast cancer by methylating KEAP1 and inhibiting ferroptosis(2023/06/01)
作者：Zhe Wang, Ruolei Li, Niuniu Ho
期刊：Journal for ImmunoTherapy of Cancer
影响因子 ：10.900
引用产品：CL-0007 CL-0041 CL-0093 CL-0149 CL-0211 CL-0228 CL-0525 CM-0007 CM-0041 CM-0093 CM-0149 CM-0211 CM-0228 CM-0525
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CircEGFR reduces the sensitivity of pirarubicin and regulates the malignant progression of triple-negative breast cancer via the miR-1299/EGFR axis(2023/06/09)
作者：Jiulong Ma, Chen Chen, Zhimin Fan
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125295
影响因子 ：8.200
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A truncated DNA aptamer with high selectivity for estrogen receptor-positive breast cancer cells(2023/08/25)
作者：Ying Cong, Shuyue Zhang, Hongmei Li
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.126450
影响因子 ：8.200
-
131I-Labeled Anti-HER2 Nanobody for Targeted Radionuclide Therapy of HER2-Positive Breast Cancer(2023/04/07)
作者：Lingzhou Zhao, Jiali Gong, Qinli Qi
期刊：International Journal of Nanomedicine
影响因子 ：8.000
引用产品：CL-0211
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Combined Molecular and Morphological Imaging of CTCs for HER2-Targeted Chemotherapy Guidance(2023/08/22)
作者：Haimeng Pei, Zhaojun Han, Dexin Du
期刊：ANALYTICAL CHEMISTRY
DOI：10.1021/acs.analchem.3c02333
影响因子 ：7.400
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Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors(2023/08/29)
作者：Yang Wei, Xu Meiyu, Xu Shuoqi, Guan Qingxian, Geng Shuaiming, Wang Juanhong, Wei Wei, Xu Hongwei, Liu Ying, Meng Yong, Gao Ming-Qing
期刊：BREAST CANCER RESEARCH
DOI：10.1186/s13058-023-01703-7
影响因子 ：7.400
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Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device(2019/08/02)
作者：Haimeng Pei, Lu Li
期刊：Anal. Chem
DOI：10.1021/acs.analchem.9b01647
影响因子 ：7.400
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An estrogen-regulated long non-coding RNA NCALD promotes luminal breast cancer proliferation by activating GRHL2(2024/01/30)
作者：Yue Meng, Dianrong Zhou, Ying Luo
期刊：Cancer Cell International
DOI：10.1186/s12935-024-03245-0
影响因子 ：5.800
引用产品：CL-0211
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Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy(2023/03/20)
作者：Tianbao Dong, Chaowei Han, Xin Liu
期刊：Molecular Pharmaceutics
DOI：10.1021/acs.molpharmaceut.2c01047
影响因子 ：4.900
引用产品：CL-0211
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Extracellular vesicles released by hypoxia-induced tumor-associated fibroblasts impart chemoresistance to breast cancer cells via long noncoding RNA H19 delivery(2024/01/10)
作者：Shuang Tao, Jian Wang, Fang Li
期刊：FASEB JOURNAL
影响因子 ：4.800
引用产品：CL-0211
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ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop(2023/06/16)
作者：Sijia Han, Xueying Jin, Tianyu Hu
期刊：CARCINOGENESIS
影响因子 ：4.700
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The miR-345-3p/PPP2CA signaling axis promotes proliferation and invasion of breast cancer cells(2021/12/18)
作者：Qian Zeng, Fangfang Jin, Husun Qian
期刊：Carcinogenesis
影响因子 ：4.700
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A comprehensive analysis of the role of QPRT in breast cancer(2023/09/18)
作者：Yiqing Yan, Lun Li, Zixin Wang
期刊：Scientific Reports
DOI：10.1038/s41598-023-42566-4
影响因子 ：4.600
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Bioinformatic analysis reveals an association between Metadherin with breast cancer prognosis and tumor immune infiltration(2024/01/23)
作者：Lixian Yang, Liu Yang, Fanting Kong
期刊：Scientific Reports
DOI：10.1038/s41598-024-52403-x
影响因子 ：4.600
引用产品：CL-0211
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CircLNPEP promotes the progression of ovarian cancer through regulating miR-876-3p/ WNT5A axis(2021/08/31)
作者：Wenwen Wang, Weiwei Zhang, Hongjun Guo
期刊：Cell Cycle
DOI：10.1080/15384101.2021.1965723
影响因子 ：4.300
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LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway(2023/02/10)
作者：Sijia Han, Xueying Jin, Tianyu Hu, Feng Chi
期刊：ONCOLOGY REPORTS
影响因子 ：4.200
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Spirocitrinols A and B, citrinin derivatives with a spiro[chromane-2,3′-isochromane] skeleton from Penicillium citrinum(2023/02/20)
作者：Junjie Tong, Yang Zhang, Yang Xu
期刊：RSC Advances
影响因子 ：3.900
引用产品：CL-0211
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Analysis of m7G-Related signatures in the tumour immune microenvironment and identification of clinical prognostic regulators in breast cancer(2023/06/23)
作者：Qinghua Huang, Jianlan Mo, Huawei Yang
期刊：BMC CANCER
DOI：10.1186/s12885-023-11012-z
影响因子 ：3.800
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CKAP2L, transcriptionally inhibited by FOXP3, promotes breast carcinogenesis through the AKT/mTOR pathway(2022/01/21)
作者：Feng Chi, Long Chen, Xiaoming Jin
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2022.113035
影响因子 ：3.700
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Development, methodological evaluation and application of a cell-based TRF assay for analysis of ADCC activity(2023/08/19)
作者：Xiao Zhu, Likun Gong, Qiuping Qin
期刊：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
DOI：10.1016/j.jpba.2023.115655
影响因子 ：3.400
引用产品：CL-0211
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Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells(2018/09/27)
作者：Zhijing Zhao, Junfeng Ma, Ying Mao
期刊：Medical Science Monitor
影响因子 ：3.100
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Identification of TFR2 as a novel ferroptosis‑related gene that serves an important role in prognosis and progression of triple‑negative breast cancer(2023/12/05)
作者：Yan Yang, Jie Du, Yunfei Huang
期刊：Oncology Letters
影响因子 ：2.900
引用产品：CL-0211
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Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer(2022/11/06)
作者：Dandan Yi, Fazhan Xu, Ru Wang, Chaoyu Jiang, Jiabo Qin, YiHsuan Lee, Xianbiao Shi, Jianfeng Sang
期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 ：2.700
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Overexpression of HOXD8 inhibits the proliferation, migration and invasion of breast cancer cells by downregulating ILP2 expression(2021/7/15)
作者：Xiaoyun Wen,Yu Chen, Xiansong Fang
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
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Effects of Chemical Fixatives on Kinetic Measurements of Biomolecular Interaction on Cell Membrane(2024/01/11)
作者：Tianbao Dong, Shengyang Wan, Yanhui Wang
期刊：JOURNAL OF MEMBRANE BIOLOGY
DOI：10.1007/s00232-024-00305-4
影响因子 ：2.400
引用产品：CL-0211
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Downregulation of BUBR1 regulates the proliferation and cell cycle of breast cancer cells and increases the sensitivity of cells to cisplatin(2023/12/04)
作者：Yiran Lu, Ruiqing Wang, Song He
期刊：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI：10.1007/s11626-023-00823-w
影响因子 ：2.100
引用产品：CL-0211
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Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3(2023/04/10)
作者：Xue Hu, Baoliang Guo, Tong Sun
期刊：Open Medicine
影响因子 ：2.100
引用产品：164210 164215 CL-0149 CL-0150 CL-0211 CL-0383 CL-0525 PB180120 PB180424 PM150140 PM150312 PM150710 PM151010
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