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产品概述
| 名称 | SK-MES-1 (人肺鳞癌细胞) (STR鉴定正确) |
| 别称 | SK MES 1; SKMES-1; SK-Mes-1; SK-MES1; SKMES1; SK-MES; SKMES |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | MEM（含NEAA）＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | SK-MES-1细胞是由G·Trempe和L·J·Old于1970年从一个患肺部鳞癌的病人胸水中分离培养。 |
| 年龄（性别） | 男；65岁 |
| 组织来源 | 肺鳞状细胞癌；转移位置：胸水 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~50 hours |
| 抗原表达情况 | Blood Type O; Rh+; HLA A3, Aw30, B7, B27 |
| 保藏机构 | ATCC; HTB-58 DSMZ; ACC-353 ECACC; 91091804ECACC; 93120837 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 12 D2S1338 19 D3S1358 16 D5S818 11 D7S820 8 D8S1179 13,14 D13S317 11 D16S539 13 D18S51 17 D19S433 14 D21S11 29,30 FGA 20,24 PentaD 12,13 PentaE 5,11 TH01 6,9.3 TPOX 8 vWA 14 D6S1043 12,19 D12S391 22 D2S441 10,14 -
STR鉴定图
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参考文献
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Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway(2021/12/07)
作者：Zhonghai Xie, Hongwei Li, Jin Zang
期刊：Bioengineered
DOI：10.1080/21655979.2021.2005931
影响因子 ：4.900
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Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating leucine zipper tumor suppressor (LZTS3) through sponging microRNA miR-1275(2020/12/31)
作者：Junpeng Feng, Jianhang Li , Peng Qie
期刊：Bioengineered
DOI：10.1080/21655979.2020.1860492
影响因子 ：4.900
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M7G-Related lncRNAs predict prognosis and regulate the immune microenvironment in lung squamous cell carcinoma(2022/11/04)
作者：Pan, Junfan, Huang, Zhidong, Lin, Hancui, Cheng, Wenfang, Lai, Jinhuo, Li, Jiancheng
期刊：BMC CANCER
DOI：10.1186/s12885-022-10232-z
影响因子 ：3.800
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TFAP2A-induced SLC2A1-AS1 promotes cancer cell proliferation(2021/2/19)
作者：Yuanbo Cui, Chunyan Zhang, Shanshan Ma
期刊：Biological Chemistry
影响因子 ：3.700
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CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway(2021/11/12)
作者：Decai Chi, Dong Wang, Minghui Zhang
期刊：Experimental Cell Research
DOI：10.1016/j.yexcr.2021.112914
影响因子 ：3.700
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Proteasome 26S subunit, non-ATPase 1 (PSMD1) facilitated the progression of lung adenocarcinoma by the de-ubiquitination and stability of PTEN-induced kinase 1 (PINK1)(2022/02/19)
作者：Lihua Liu, Aihua Liu, Jun Dong
期刊：Experimental Cell Research
DOI：10.1016/j.yexcr.2022.113075
影响因子 ：3.700
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circRNA-mediated upregulation of HOXC9 is correlated with poor outcome and immune microenvironment infiltrates in LUAD(2022/10/14)
作者：Yunlong Liu, Liling Jing, Jie Zhang
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.10.043
影响因子 ：3.100
