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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Human

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  • Procell-FGF-2/ bFGF/ FGF-b (K128N), Human, Recombinant

FGF-2/ bFGF/ FGF-b (K128N), Human, Recombinant

货号:PCK005

价格:¥980¥1880¥7880¥11180

规格:
10µg
  • 10µg
  • 50µg
  • 500µg
  • 1mg
数量: - +
产品概述

产品信息

别名 Fibroblast Growth Factor 2; FGF-2; Basic fibroblast Growth Factor; bFGF; Heparin-binding Growth Factor 2; HBGF-2; FGF2; FGFB
物种 Human
表达宿主 E.coli
序列信息 Met1-Ser155
检索号 BAG70135.1
分子量 17kDa
表观分子量 17.2 kDa
生物活性 Testing in progress

产品特性

纯度 >95% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU/µg as determined by LAL test.
保存 Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled waterPlease aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

Fibroblast Growth Factors (FGF) are a family of heparin-binding secreted Proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.
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