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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Mouse

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  • Procell-FGF-2/ bFGF/ FGF-b (Met1-Ser154), Mouse, Recombinant

FGF-2/ bFGF/ FGF-b (Met1-Ser154), Mouse, Recombinant

货号:PCK011

价格:¥780¥1680¥7080¥9980

规格:
10µg
  • 10µg
  • 50µg
  • 500µg
  • 1mg
数量: - +
产品概述

产品信息

别名 Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; Fgf2; Fgf-2
物种 Mouse
表达宿主 E.coli
序列信息 Met1-Ser154
检索号 P15655
分子量 17.2 kDa
表观分子量 16 kDa
生物活性 Measured in a cell proliferation assay using BALB/ c 3T3 cells.The ED50 for this effect is less than 2 ng/ ml.

产品特性

纯度 >95% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 20mM PB, 400mM NaCl, pH 7.0.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

FGF basic is one of 22 mitogenic Proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF Receptors. FGF basic binds other Proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions.
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