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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Human

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  • Procell-VEGF-A/ VEGF165, Human, Recombinant

VEGF-A/ VEGF165, Human, Recombinant

货号:PCK015

价格:¥1780¥4980¥19780¥28880

规格:
10µg
  • 10µg
  • 50µg
  • 500µg
  • 1mg
数量: - +
产品概述

产品信息

别名 Vascular Endothelial Growth Factor Isoform 165; VEGF165
物种 Human
表达宿主 Human Cells
序列信息 Ala27-Arg191
检索号 P15692-4
分子量 19.1 kDa
表观分子量 20 kDa
生物活性 Loaded Human VEGF-165 Antibody(12E3)-mFcon AMQ Biosensor, can bind Human VEGF165 with an affinity constant of <10-3 nM as determined in BLI assay.Loaded Human VEGF-165 Antibody(10F9)-mFc on AMQ Biosensor, can bind Human VEGF165 with an affinity constant of 0.004 nM as determined in BLI assay.

产品特性

纯度 >90% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

Human Vascular Endothelial Growth Factor (VEGF), also known as VEGF-A and vascular Permeability Factor (VPF), belongs to the platelet-Derived Growth Factor family of cysteine-knot Growth Factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase Receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
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