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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Human

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  • Procell-GM-CSF/ CSF2, Human, Recombinant

GM-CSF/ CSF2, Human, Recombinant

货号:PCK036

价格:¥1380¥3680¥15880¥23280

规格:
10µg
  • 10µg
  • 50µg
  • 500µg
  • 1mg
数量: - +
产品概述

产品信息

别名 Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
物种 Human
表达宿主 P.pastoris
序列信息 Ala18-Glu144
检索号 P04141
分子量 14.4 kDa
表观分子量 24-35 kDa
生物活性 Measured in a cell proliferation assay using TF‑1 human erythroleukemic cellsThe ED50 for this effect is less than 0.6 ng/ mL.

产品特性

纯度 >90% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

GM-CSF was initially characterized as a Growth Factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to Cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a Growth Factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages, and eosinophils, GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
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