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当前位置:首页 >> 产品中心 >> 辅助试剂 >> 细胞因子 >> Human

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  • Procell-TNF-α/ TNFA/ TNFSF2 (C-6His), Human, Recombinant

TNF-α/ TNFA/ TNFSF2 (C-6His), Human, Recombinant

货号:PCK051

价格:¥680¥1380¥6880¥9980

规格:
10µg
  • 10µg
  • 50µg
  • 500µg
  • 1mg
数量: - +
产品概述

产品信息

别名 Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
物种 Human
表达宿主 E.coli
序列信息 Val77-Leu233
检索号 P01375
分子量 18.5 kDa
表观分子量 16 kDa
标签 C-6His
生物活性 Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less than 0.1 ng/ ml.

产品特性

纯度 >95% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled waterPlease aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons, IL2, and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF Receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8, Lys9, Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
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