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产品概述
产品信息
| 别名 | Glial Cell Line-Derived Neurotrophic Factor; hGDNF; Astrocyte-Derived Trophic Factor; ATF; GDNF |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Ser78-Ile211 |
| 检索号 | P39905 |
| 分子量 | 15.1 kDa |
| 表观分子量 | 17 kDa |
| 生物活性 | Measured by its ability to bind Human GFRA1 in functional ELISA. Activated |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| 运输 | The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.25. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glyco Protein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric Protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.
