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产品概述
产品信息
| 别名 | Ciliary Neurotrophic Factor; CNTF |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Ala2-Met200 |
| 检索号 | P26441 |
| 分子量 | 22.93 kDa |
| 表观分子量 | 25 kDa |
| 生物活性 | Testing in progress |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| 运输 | The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM Cysteine, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their Protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle Cytokines share gp130 as a signal transducing subunit in their Receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-Derived endothelial cell Growth Factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.
