Exodus-2/CCL21a, Mouse, Recombinant
英文名称：Exodus-2/CCL21a, Mouse, Recombinant
货号： PCK229
价格： ￥11880 ￥2080 ￥780 ￥17280
产品信息
| 产品形态 | 粉末 |
| 产品规格 | 500μg / 50μg / 10μg / 1mg |
| 别名 | C-C Motif Chemokine 21a;6Ckine;Beta-Chemokine Exodus-2;Small-Inducible Cytokine A21a;Thymus-Derived Chemotactic Agent 4;TCA4;Ccl21a;Scya21;Scya21a |
| 物种 | 小鼠 |
| 表达宿主 | E.coli |
| 序列信息 | Ser24-Gly133 |
| 检索号 | P84444 |
| 分子量 | 12.1 kDa |
| 表观分子量 | 16 kDa |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
| 有效期 | 12个月 |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介绍 | C-C Motif Chemokine 21a (CCL21a) is a small secreted Cytokine that belongs to the intercrine beta (CC Chemokine) family. Mouse CCL21 cDNA encodes a 133 amino acid residue Protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature Protein. Mouse CCL21 has three forms while CCL21a has Ser-65. CCL21 elicits its effects by binding to a cell surface Chemokine Receptor known as CCR7 and CXCR3. Mouse CCL21 inhibits hemopoiesis and stimulates chemotaxis. It has chemotactic function in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Mouse CCL21 shows preferential activity towards naive T-cells and may play a role in mediating homing of lymphocytes to secondary lymphoid organs. |
-
Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
-
Q2:细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？
如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱
-
Q3:细胞因子加无菌水溶解后需要再次离心吗？
加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的
-
Q4:细胞因子离心前需要放置室温吗？
不用提前放置室温.
识别码示意图
