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产品概述
产品简介
Dulbecco's改良培养基——DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L(低糖型)，后来又发展出葡萄糖含量为4500mg/L(高糖型)，现已广泛应用于各种细胞的培养。DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。
产品说明
| 产品形态 | 液体 |
| 产品浓度 | 1× |
| 产品规格 | 125mL×4 |
| 培养基成分 | DMEM高糖(PM150210)＋10% FBS(164210-50)＋1% P/S(PB180120)＋1% GlutaMax(PB180419) |
| 细菌检测 | 阴性 |
| 真菌检测 | 阴性 |
| 支原体检测 | 阴性 |
| 细胞生长实验 | 细胞生长良好，形态正常 |
| 内毒素含量(EU/mL) | ≤3 |
| 储存条件 | 2~8℃，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 3个月 |
注意事项
1、使用产品时应注意无菌操作，避免污染。 2、本产品含有血清和双抗，如无特别需要不用额外再添加血清和双抗，可以直接使用。 3、为保持本产品的最佳使用效果，不宜将其长时间放置于室温或较高的温度环境中。 4、所有产品请于保质期内使用，超出保质期，必须放弃使用。 5、本产品只供进一步科研使用，不可应用于临床等其他方面。
参考文献
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Hyperthermia-triggered biomimetic bubble nanomachines(2023/08/11)
作者：Junbin Gao, Hanfeng Qin, Fei Wang
期刊：Nature Communications
DOI：10.1038/s41467-023-40474-9
影响因子 ：16.600
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A novel pH-sensitive targeting polysaccharide-gold nanorod conjugate for combined photothermal-chemotherapy of breast cancer(2019/02/13)
作者：Guanghui Hou, Junmin Qian, Weijun Xu
期刊：Carbohydrate Polymers
DOI：10.1016/j.carbpol.2019.02.045
影响因子 ：11.200
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Preparation, characterization, and biocompatibility evaluation of poly(Nɛ-acryloyl-l-lysine)/hyaluronic acid interpenetrating network hydrogels(2015/09/26)
作者：Ning Cui, Junmin Qian, Weijun Xu
期刊：Carbohydrate Polymers
DOI：10.1016/j.carbpol.2015.09.095
影响因子 ：11.200
引用产品：PM150210B
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Doxorubicin/cisplatin co-loaded hyaluronic acid/chitosan-based nanoparticles for in vitro synergistic combination chemotherapy of breast cancer(2019/08/15)
作者：Yaping Wang, Junmin Qian, Ming Yang
期刊：Carbohydrate Polymers
DOI：10.1016/j.carbpol.2019.115206
影响因子 ：11.200
引用产品：PM150210B
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Biomechanically-adapted immunohydrogels reconstructing myelin sheath for peripheral nerve regeneration(2022/08/03)
作者：Yifan Wang, Zhiwen Yan, Wenjun Liu
期刊：Advanced Healthcare Materials
影响因子 ：10.000
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ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer(2022/08/20)
作者：Chen Zhou, Shoukang Li, Kaijian Bin
期刊：CANCER LETTERS
DOI：10.1016/j.canlet.2022.215855
影响因子 ：9.700
引用产品：PM150210B
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A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer(2018/11/18)
作者：Weijun Xu, Junmin Qian, Guanghui Hou
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.11.026
影响因子 ：9.300
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PEGylated hydrazided gold nanorods for pH-triggered chemo/photodynamic/photothermal triple therapy of breast cancer(2018/10/12)
作者：Weijun Xu, Junmin Qian, Guanghui Hou
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.10.019
影响因子 ：9.300
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A double-network poly(Nɛ-acryloyl l-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment(2016/01/20)
作者：Weijun Xu, Junmin Qian, Guanghui Hou
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2016.01.027
影响因子 ：9.300
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Protective effect of sodium butyrate on intestinal barrier damage and uric acid reduction in hyperuricemia mice(2023/03/20)
作者：Yukun Li, Hanqing Li, Rong Wang
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114568
影响因子 ：7.500
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Ten-gene signature reveals the significance of clinical prognosis and immuno-correlation of osteosarcoma and study on novel skeleton inhibitors regarding MMP9(2021/07/14)
作者：Weihang Li, Ziyi Ding, Dong Wang
期刊：Cancer Cell International
DOI：10.1186/s12935-021-02041-4
影响因子 ：5.800
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Antibacterial MccM as the Major Microcin in Escherichia coli Nissle 1917 against Pathogenic Enterobacteria(2023/07/20)
作者：Yi Ma, Wei Fu, Bin Hong
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：5.600
引用产品：PM150210B
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Aucubin improves chronic unpredictable mild stress-induced depressive behavior in mice via the GR/NF-κB/NLRP3 axis(2023/07/29)
作者：Ping Liu, Shiyuan Song, Ping Yang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
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影响因子 ：5.600
引用产品：PM150210B
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Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection(2021/05/04)
作者：Qihe Tang, Qingyu Wang, Zhuojian Sun
期刊：Frontiers in Pharmacology
影响因子 ：5.600
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Circ_0001861 facilitates trophoblast cell proliferation, migration, invasion and epithelial-mesenchymal transition via the miR-296-5p/forkhead box protein 1 pathway in preeclampsia(2023/12/29)
作者：Yanhua Liu, Shengpu Wang, Xiaoying Zhang
期刊：JOURNAL OF HYPERTENSION
DOI：10.1097/HJH.0000000000003634
影响因子 ：4.900
引用产品：PM150210B
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Osteoblast Jmjd3 regulates osteoclastogenesis via EphB4 and RANKL signalling(2022/02/18)
作者：Rui Wang, Huikun Luo, Yang Di
期刊：Oral Diseases
影响因子 ：3.800
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Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma(2021/09/30)
作者：Xiaoyu Zhang, Zhuochang Chen, Nan Li
期刊：Human Molecular Genetics
影响因子 ：3.500
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miR-339-5p Inhibits Autophagy to Reduce the Resistance of Laryngeal Carcinoma on Cisplatin via Targeting TAK1(2021/8/3)
作者：Guang Li, Zexing Cheng
期刊：Biomed Research International
影响因子 ：3.246
引用产品：PM150210B
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Circ_0001806 relieves LPS-induced HK2 cell injury by regulating the expression of miR-942-5p and TXNIP(2023/08/05)
作者：Mingjin Chen, Lefeng Zhang
期刊：JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
DOI：10.1007/s10863-023-09978-3
影响因子 ：3.000
引用产品：PM150210B
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M2 macrophages-derived exosomes regulate osteoclast differentiation by the CSF2/TNF-α axis(2024/01/18)
作者：Yue Zhou, Guangyao Hu
期刊：BMC Oral Health
DOI：10.1186/s12903-023-03842-x
影响因子 ：2.900
引用产品：PM150210B
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In vitro facilitating role of polygonatum sibiricum polysaccharide in osteogenic differentiation of bone marrow mesenchymal stem cells from patients with multiple myeloma(2021/04/23)
作者：Jianqiang Zhao, Lijie Ma, Zengfeng Ni
期刊：Biotechnology Letters
DOI：10.1007/s10529-021-03125-x
影响因子 ：2.700
引用产品：PM150210B
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Systematic understanding of the potential targets and pharmacological mechanisms of acteoside by network pharmacology approach(2020/02/25)
作者：Yiliyasi Aisa, Kurexi Yunusi, Qian Chen
期刊：Medicinal Chemistry Research
DOI：10.1007/s00044-020-02524-5
影响因子 ：2.600
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Regulatory mechanism of DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes in prostate cancer cell proliferation and apoptosis(2023/03/24)
作者：Xiao Wu, Zhongyi Zeng, Kai Peng
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2023.102078
影响因子 ：2.600
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Artesunate restrains the malignant progression of human cutaneous squamous cell carcinoma cells via the suppression of the PI3K/AKT pathway(2022/03/19)
作者：Xinyan Huang, Songzhao Zhang, Wenxi Wang
期刊：Tissue & Cell
DOI：10.1016/j.tice.2022.101789
影响因子 ：2.600
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Circ_0044516 Enriches the Level of SARM1 as a miR-330-5p Sponge to Regulate Cell Malignant Behaviors and Tumorigenesis of Prostate Cancer(2022/01/07)
作者：Yan Wu
期刊：Biochemical Genetics
DOI：10.1007/s10528-021-10160-w
影响因子 ：2.400
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Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway(2023/03/01)
作者：Haixu Wang, Yan Li, Chuang Liu
期刊：JOURNAL OF TOXICOLOGICAL SCIENCES
影响因子 ：2.000
引用产品：PM150210B
