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产品概述
产品简介
Dulbecco's改良培养基——DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素的含量增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基维生素的含量增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L(低糖型),后来又发展出葡萄糖含量为4500mg/L(高糖型),现已广泛应用于各种细胞的培养。DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产,例如利用CHO细胞表达EPO和生产乙肝疫苗。
产品说明
产品形态 | 液体 |
产品浓度 | 1× |
产品规格 | 125mL×4 |
培养基成分 | DMEM高糖(PM150210)+10% FBS(164210-50)+1% P/S(PB180120)+1% GlutaMax(PB180419) |
细菌检测 | 阴性 |
真菌检测 | 阴性 |
支原体检测 | 阴性 |
细胞生长实验 | 细胞生长良好,形态正常 |
内毒素含量(EU/mL) | ≤3 |
储存条件 | 2~8℃,避光储存 |
运输条件 | 冰袋冷藏运输 |
有效期 | 3个月 |
注意事项
1、使用产品时应注意无菌操作,避免污染。 2、本产品含有血清和双抗,如无特别需要不用额外再添加血清和双抗,可以直接使用。 3、为保持本产品的最佳使用效果,不宜将其长时间放置于室温或较高的温度环境中。 4、所有产品请于保质期内使用,超出保质期,必须放弃使用。 5、本产品只供进一步科研使用,不可应用于临床等其他方面。
参考文献
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A novel pH-sensitive targeting polysaccharide-gold nanorod conjugate for combined photothermal-chemotherapy of breast cancer(2019/02/13)
作者:Guanghui Hou, Junmin Qian, Weijun Xu
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2019.02.045
影响因子 :11.200
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Preparation, characterization, and biocompatibility evaluation of poly(Nɛ-acryloyl-l-lysine)/hyaluronic acid interpenetrating network hydrogels(2015/09/26)
作者:Ning Cui, Junmin Qian, Weijun Xu
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2015.09.095
影响因子 :11.200
引用产品:PM150210B
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Doxorubicin/cisplatin co-loaded hyaluronic acid/chitosan-based nanoparticles for in vitro synergistic combination chemotherapy of breast cancer(2019/08/15)
作者:Yaping Wang, Junmin Qian, Ming Yang
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2019.115206
影响因子 :11.200
引用产品:PM150210B
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Biomechanically-adapted immunohydrogels reconstructing myelin sheath for peripheral nerve regeneration(2022/08/03)
作者:Yifan Wang, Zhiwen Yan, Wenjun Liu
期刊:Advanced Healthcare Materials
影响因子 :10.000
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ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer(2022/08/20)
作者:Chen Zhou, Shoukang Li, Kaijian Bin
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2022.215855
影响因子 :9.700
引用产品:PM150210B
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A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer(2018/11/18)
作者:Weijun Xu, Junmin Qian, Guanghui Hou
期刊:Acta Biomaterialia
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影响因子 :9.300
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PEGylated hydrazided gold nanorods for pH-triggered chemo/photodynamic/photothermal triple therapy of breast cancer(2018/10/12)
作者:Weijun Xu, Junmin Qian, Guanghui Hou
期刊:Acta Biomaterialia
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影响因子 :9.300
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A double-network poly(Nɛ-acryloyl l-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment(2016/01/20)
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期刊:Acta Biomaterialia
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Protective effect of sodium butyrate on intestinal barrier damage and uric acid reduction in hyperuricemia mice(2023/03/20)
作者:Yukun Li, Hanqing Li, Rong Wang
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.114568
影响因子 :7.500
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Ten-gene signature reveals the significance of clinical prognosis and immuno-correlation of osteosarcoma and study on novel skeleton inhibitors regarding MMP9(2021/07/14)
作者:Weihang Li, Ziyi Ding, Dong Wang
期刊:Cancer Cell International
DOI:10.1186/s12935-021-02041-4
影响因子 :5.800
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Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection(2021/05/04)
作者:Qihe Tang, Qingyu Wang, Zhuojian Sun
期刊:Frontiers in Pharmacology
影响因子 :5.600
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Osteoblast Jmjd3 regulates osteoclastogenesis via EphB4 and RANKL signalling(2022/02/18)
作者:Rui Wang, Huikun Luo, Yang Di
期刊:Oral Diseases
影响因子 :3.800
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Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma(2021/09/30)
作者:Xiaoyu Zhang, Zhuochang Chen, Nan Li
期刊:Human Molecular Genetics
影响因子 :3.500
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miR-339-5p Inhibits Autophagy to Reduce the Resistance of Laryngeal Carcinoma on Cisplatin via Targeting TAK1(2021/8/3)
作者:Guang Li, Zexing Cheng
期刊:Biomed Research International
影响因子 :3.246
引用产品:PM150210B
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A new monoterpene alkaloid from Incarvillea Sinensis with migration inhibitory activity on cancer cell(2023/03/09)
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期刊:CHEMISTRY & BIODIVERSITY
影响因子 :2.900
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In vitro facilitating role of polygonatum sibiricum polysaccharide in osteogenic differentiation of bone marrow mesenchymal stem cells from patients with multiple myeloma(2021/04/23)
作者:Jianqiang Zhao, Lijie Ma, Zengfeng Ni
期刊:Biotechnology Letters
DOI:10.1007/s10529-021-03125-x
影响因子 :2.700
引用产品:PM150210B
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Systematic understanding of the potential targets and pharmacological mechanisms of acteoside by network pharmacology approach(2020/02/25)
作者:Yiliyasi Aisa, Kurexi Yunusi, Qian Chen
期刊:Medicinal Chemistry Research
DOI:10.1007/s00044-020-02524-5
影响因子 :2.600
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Regulatory mechanism of DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes in prostate cancer cell proliferation and apoptosis(2023/03/24)
作者:Xiao Wu, Zhongyi Zeng, Kai Peng
期刊:TISSUE & CELL
DOI:10.1016/j.tice.2023.102078
影响因子 :2.600
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Artesunate restrains the malignant progression of human cutaneous squamous cell carcinoma cells via the suppression of the PI3K/AKT pathway(2022/03/19)
作者:Xinyan Huang, Songzhao Zhang, Wenxi Wang
期刊:Tissue & Cell
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影响因子 :2.600
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Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway(2023/03/01)
作者:Haixu Wang, Yan Li, Chuang Liu
期刊:JOURNAL OF TOXICOLOGICAL SCIENCES
影响因子 :2.000
引用产品:PM150210B