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产品概述
| 名称 | 5637 (人膀胱癌细胞) (STR鉴定正确) |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 注意事项 | 该细胞较难消化，注意延长消化时间，消化到细胞收缩变圆，轻拍培养瓶侧边，细胞可以滑落方可终止消化。 |
| 背景描述 | 5637细胞能生成SCF、IL-1、IL-3、IL-6、G-CSF、GM-CSF等。 |
| 年龄（性别） | 男；68岁 |
| 组织来源 | 膀胱；癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 膀胱癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~24-36 hours |
| 致瘤性 | Yes, within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
| 保藏机构 | ATCC; HTB-9 DSMZ; ACC-35 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 25 D3S1358 15,17 D5S818 11,12 D7S820 10,11 D8S1179 10,16 D13S317 11 D16S539 9 D18S51 16,18 D19S433 13,15 D21S11 36 FGA 22 PentaD 11 PentaE 10,12 TH01 7,9 TPOX 8 vWA 18 D6S1043 16,20 D12S391 20 D2S441 14 -
STR鉴定图
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参考文献
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Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D(2018/11/27)
作者：Zhe Qiang, Zongyuan Zhou, Ting Peng
期刊：Journal Of Experimental & Clinical Cancer Research
影响因子 ：11.300
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity(2023/01/07)
作者：Xiaosong Wei, Beibei Wang, Zixin Wu
期刊：CANCER LETTERS
DOI：10.1016/j.canlet.2023.216058
影响因子 ：9.700
引用产品：CL-0002 CL-0125 CL-0222 CL-0227 CL-0449A CL-0463 CL-0628 PM150410 PM150710 PM150910 PM151010
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Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.(2022/09/21)
作者：Longfei Yang, Yuwei Zhang, Yang Wang
期刊：Frontiers in Pharmacology
影响因子 ：5.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI(2020/03/03)
作者：Xiaosong Wei, Beibei Wang, Qi Wang
期刊：Frontiers in Pharmacology
影响因子 ：5.600
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CRTAC1 (Cartilage acidic protein 1) inhibits cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process in bladder cancer by downregulating Yin Yang 1 (YY1) to inactivate the TGF-β pathway(2021/11/24)
作者：Jianghua Yang, Li Fan, Xiaoxing Liao
期刊：Bioengineered
DOI：10.1080/21655979.2021.1974645
影响因子 ：4.900
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The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis(2022/11/21)
作者：Jiani He, Changming Dong, Hao Zhang, Yuanjun Jiang, Tao Liu, Xiaojun Man
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2022.110527
影响因子 ：4.800
引用产品：CL-0002 CL-0005 CL-0122 CL-0125 CL-0222 CL-0227 CL-0431
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A pan-cancer analysis of DDR1 in prognostic signature and tumor immunity, drug resistance(2023/04/08)
作者：Longfei Yang, Yuwei Zhang, Yifan Tang
期刊：Scientific Reports
DOI：10.1038/s41598-023-27975-9
影响因子 ：4.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway( 2022/01/02)
作者：Bo Li, Qian Xiao, Liping Shan
期刊：Cell Cycle
DOI：10.1080/15384101.2021.2021050
影响因子 ：4.300
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CircRPPH1 accelerates the proliferation and migration of bladder cancer via enhancing the STAT3 signaling pathway(2023/03/31)
作者：Xiao Liu, Yonghua Tong, Qiu Huang
期刊：ONCOLOGY REPORTS
影响因子 ：4.200
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LncRNA MBNL1-AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/PHLPP2/FOXO1 axis in bladder cancer(2019/11/25)
作者：Xiaosong Wei, Xiaoming Yang, Beibei Wang
期刊：Cancer Medicine
影响因子 ：4.000
引用产品：CL-0002
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Rad54L promotes bladder cancer progression by regulating cell cycle and cell senescence(2022/09/07)
作者：Yinzhao Wang, Tailai Zhou, Hengxing Chen
期刊：MEDICAL ONCOLOGY
DOI：10.1007/s12032-022-01751-7
影响因子 ：3.400
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HEATR3 involved in the cell proliferation, metastasis and cell cycle development of bladder cancer acts as a tumor suppressor(2023/07/31)
作者：Xia Dou, Xiaoya Ma, Wenting Meng
期刊：MOLECULAR GENETICS AND GENOMICS
DOI：10.1007/s00438-023-02046-w
影响因子 ：3.100
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Effect of isorhamnetin on carbonic anhydrase IX expression and tumorigenesis of bladder cancer by activating PPARγ/PTEN/AKT pathway(2023/03/02)
作者：Peng Zhang, Yisheng Sun, Lei Shi
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2023.102048
影响因子 ：2.600
引用产品：CL-0002
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Hsa_circ_0008035 Knockdown Inhibits Bladder Cancer Progression through miR-1184/RAP2B Axis(2023/03/23)
作者：Yang Fu, Kun Liu, Xi Jiang
期刊：UROLOGIA INTERNATIONALIS
影响因子 ：1.600
