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产品概述
名称 | U-2 OS [U2OS] (人骨肉瘤细胞) (STR鉴定正确) |
别称 | U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T |
背景描述 | U-2 OS细胞(原名2T)是由Ponten·J和Saksela·E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立的。U-2 OS细胞与WI-38细胞共培养或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的补体结合试验(CF法)未检测到病毒。U-2 OS细胞表达胰岛素样生长因子I、II(IGF-I、IGF-II)受体和骨肉瘤衍生生长因子(ODGF)。 |
种属 | 人 |
年龄(性别) | 女 |
组织来源 | 骨;骨肉瘤 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | 1 |
生长培养基 | McCoy's 5A+10% FBS+1% P/S |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~25-30 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II) |
抗原表达情况 | Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
基因表达情况 | osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
保藏机构 | ATCC; HTB-96 DSMZ; ACC-785 ECACC; 92022711 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13 D2S1338 20,24 D3S1358 16 D5S818 11 D7S820 11,12 D8S1179 12,14 D13S317 13 D16S539 11,12 D18S51 14 D19S433 15 D21S11 31 FGA 20,21 PentaD 9 PentaE 10,13 TH01 6,9.3 TPOX 11,12 vWA 14,18 D6S1043 11 D12S391 19,20 D2S441 10,14 -
STR鉴定图
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参考文献
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Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase(2021/08/05)
作者:Ximing Shao, Zhihao Ding, Wenhua Zhou
期刊:Nature Nanotechnology
DOI:10.1038/s41565-021-00952-x
影响因子 :40.523
引用产品:CL-0016 CL-0101 CL-0103 CL-0150A CL-0150B CL-0236 CL-0238
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Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor(2022/09/22)
作者:Haoyu Guo, Lutong Wang, Wei Wu
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.09.020
影响因子 :11.4670
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MoS2 Quantum Dot@Polyaniline Inorganic–Organic Nanohybrids for in Vivo Dual-Modal Imaging Guided Synergistic Photothermal/Radiation Therapy(2016/09/07)
作者:Jinping Wang, Xiaoxiao Tan, Xiaojuan Pang
期刊:ACS Applied Materials & Interfaces
影响因子 :10.3830
引用产品:CL-0016 CL-0101 CL-0103 CL-0150A CL-0150B CL-0236 CL-0238
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lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression(2021/08/26)
作者:Lei Ding, Taiyuan Liu, Yuan Qu
期刊:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2021.08.022
影响因子 :10.183
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Targeting visualization of malignant tumor based on the alteration of DWI signal generated by hTERT promoter–driven AQP1 overexpression(2022/01/19)
作者:Liang Zhang, Mingfu Gong, Sheng Lei
期刊:European Journal Of Nuclear Medicine And Molecular Imaging
DOI:10.1007/s00259-022-05684-1
影响因子 :10.0570
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Zinc finger Asp-His-His-Cys palmitoyl -acyltransferase 19 accelerates tumor progression through wnt/β-catenin pathway and is upregulated by miR-940 in osteosarcoma(2022/03/17)
作者:Shuhong Liang, Xiaojian Zhang, Jun Li
期刊:Bioengineered
DOI:10.1080/21655979.2022.2040827
影响因子 :6.8320
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A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma.(2022/11/11)
作者:Xinxing Wang, Guang Xia, Shilang Xiao, Song Wu, Lina Zhang, Junjie Huang, Wenxiu Zhang, Xu Cao, Image_1.tif, Image_2.tif, Image_3.tif, Image_4.tif, Image_5.tif, Image_6.tif, Image_7.tif, DataSheet_1.csv, DataSheet_2.csv, DataSheet_3.csv, DataSheet_4.
期刊:Frontiers in Oncology
影响因子 :5.7380
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma(2021/12/22)
作者:Bing Wang, Xiaowei Wang, Xing Tong
期刊:Frontiers in Genetics
影响因子 :4.772
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway(2021/09/13)
作者:Ke Li, Hongtao Yu, Chunbing Zhao
期刊:Journal of Cancer
影响因子 :4.478
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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells(2022/11/08)
作者:Zhengjiang Li, Yan Zhang, Yongkui Li, Shuxing Xing, Shunqiang Li, Jing Lyu, Zhaonan Ban
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7510