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产品概述
名称 | U-2 OS [U2OS] (人骨肉瘤细胞) (STR鉴定正确) |
别称 | U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | McCoy's 5A+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
背景描述 | U-2 OS细胞(原名2T)是由Ponten·J和Saksela·E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立的。U-2 OS细胞与WI-38细胞共培养或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的补体结合试验(CF法)未检测到病毒。U-2 OS细胞表达胰岛素样生长因子I、II(IGF-I、IGF-II)受体和骨肉瘤衍生生长因子(ODGF)。 |
年龄(性别) | 女 |
组织来源 | 骨;骨肉瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | 1 |
倍增时间 | ~25-30 hours |
受体表达情况 | insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II) |
抗原表达情况 | Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
基因表达情况 | osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
保藏机构 | ATCC; HTB-96 DSMZ; ACC-785 ECACC; 92022711 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13 D2S1338 20,24 D3S1358 16 D5S818 11 D7S820 11,12 D8S1179 12,14 D13S317 13 D16S539 11,12 D18S51 14 D19S433 15 D21S11 31 FGA 20 PentaD 9 PentaE 10,13 TH01 6,9.3 TPOX 11,12 vWA 14,18 D6S1043 11 D12S391 19,20 D2S441 10,14 -
STR鉴定图
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参考文献
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Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase(2021/08/05)
作者:Ximing Shao, Zhihao Ding, Wenhua Zhou
期刊:Nature Nanotechnology
DOI:10.1038/s41565-021-00952-x
影响因子 :38.300
引用产品:CL-0016 CL-0101 CL-0103 CL-0150A CL-0150B CL-0236 CL-0238
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Recruiting Rate Determines the Blinking Propensity of Rhodamine Fluorophores for Super-Resolution Imaging(2023/02/23)
作者:Ying Zheng, Zhiwei Ye, Xue Zhang
期刊:Journal of the American Chemical Society
影响因子 :15.000
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Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor(2022/09/22)
作者:Haoyu Guo, Lutong Wang, Wei Wu
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.09.020
影响因子 :10.800
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor(2023/01/05)
作者:Wei Huang, Xiaoyue Wu, Yifan Zhao
期刊:ACS Applied Materials & Interfaces
影响因子 :9.500
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Targeting visualization of malignant tumor based on the alteration of DWI signal generated by hTERT promoter–driven AQP1 overexpression(2022/01/19)
作者:Liang Zhang, Mingfu Gong, Sheng Lei
期刊:European Journal Of Nuclear Medicine And Molecular Imaging
DOI:10.1007/s00259-022-05684-1
影响因子 :9.100
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lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression(2021/08/26)
作者:Lei Ding, Taiyuan Liu, Yuan Qu
期刊:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2021.08.022
影响因子 :8.800
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Subtle Structural Translation Magically Modulates the Super-Resolution Imaging of Self-Blinking Rhodamines(2023/02/14)
作者:Ying Zheng, Zhiwei Ye, Yi Xiao
期刊:Analytical Chemistry
DOI:10.1021/acs.analchem.2c05298
影响因子 :7.400
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2-Hydroxy-3-methylanthraquinone inhibits homologous recombination repair in osteosarcoma through the MYC-CHK1-RAD51 axis(2023/01/30)
作者:Doudou Jing, Xuanzuo Chen, Zhenhao Zhang
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00611-y
影响因子 :5.700
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Solasonine Inhibits Cancer Stemness and Metastasis by Modulating Glucose Metabolism via Wnt/β-Catenin/Snail Pathway in Osteosarcoma(2023/06/28)
作者:Bangjun Wang, Yi Zhou, Peng Zhang
期刊:AMERICAN JOURNAL OF CHINESE MEDICINE
影响因子 :5.700
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MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1(2023/06/29)
作者:Fan Yang, Jin Wu, Mindie Zhao
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.600
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Circular RNA hsa_circ_001350 contributes to osteosarcoma progression by regulating microRNA-578/CCR4-NOT transcription complex and subunit 7/Wnt signaling(2023/06/15)
作者:Rujin Xu, Xingyuan Ci, Fang He
期刊:American Journal of Cancer Research
DOI:PMID:37424831
影响因子 :5.300
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Zinc finger Asp-His-His-Cys palmitoyl -acyltransferase 19 accelerates tumor progression through wnt/β-catenin pathway and is upregulated by miR-940 in osteosarcoma(2022/03/17)
作者:Shuhong Liang, Xiaojian Zhang, Jun Li
期刊:Bioengineered
DOI:10.1080/21655979.2022.2040827
影响因子 :4.900
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Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment(2023/06/08)
作者:Song Liao, Jianxiong Li, Song Gao
期刊:Frontiers in Oncology
影响因子 :4.700
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A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma.(2022/11/11)
作者:Xinxing Wang, Guang Xia, Shilang Xiao, Song Wu, Lina Zhang, Junjie Huang, Wenxiu Zhang, Xu Cao, Image_1.tif, Image_2.tif, Image_3.tif, Image_4.tif, Image_5.tif, Image_6.tif, Image_7.tif, DataSheet_1.csv, DataSheet_2.csv, DataSheet_3.csv, DataSheet_4.
期刊:Frontiers in Oncology
影响因子 :4.700
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Prognosis-related VDAC1 regulates the proliferation and apoptosis of osteosarcoma cells via the MAPK signaling pathway(2023/03/05)
作者:Xu Cai, Shaihong Zhu
期刊:Genomics
DOI:10.1016/j.ygeno.2023.110595
影响因子 :4.400
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Identification of an EMT-related gene-based prognostic signature in osteosarcoma(2023/04/27)
作者:Haoli Gong, Ye Tao, Sheng Xiao
期刊:Cancer Medicine
影响因子 :4.000
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway(2021/09/13)
作者:Ke Li, Hongtao Yu, Chunbing Zhao
期刊:Journal of Cancer
影响因子 :3.900
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A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction(2023/06/07)
作者:Junwei Pan, Ming Liu, Xiaozong Duan
期刊:PLoS One
DOI:10.1371/journal.pone.0286422
影响因子 :3.700
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma(2021/12/22)
作者:Bing Wang, Xiaowei Wang, Xing Tong
期刊:Frontiers in Genetics
影响因子 :3.700
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Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR-194-5p and elevating HK2 expression(2023/02/21)
作者:Dawei Wang, Yang Wang, Huadong Wang
期刊:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子 :2.900
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LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages(2023/03/28)
作者:Peng Yuan, Yukang Que, Yulei Liu
期刊:Oncology Letters
影响因子 :2.900
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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells(2022/11/08)
作者:Zhengjiang Li, Yan Zhang, Yongkui Li, Shuxing Xing, Shunqiang Li, Jing Lyu, Zhaonan Ban
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
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DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway(2023/02/27)
作者:Mingming Yang, Hang Zhang, Shichang Gao
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-03572-4
影响因子 :2.600
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Piperine improves the sensitivity of osteosarcoma cells to doxorubicin by inducing apoptosis and inhibiting the PI3K/AKT/GSK-3β pathway(2023/03/09)
作者:Yubin Qi, Lin Yao, Jianke Liu
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-03642-7
影响因子 :2.600
引用产品:CL-0236
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Circ_0003732 promotes osteosarcoma progression through regulating miR-377-3p/CPEB1 axis and Wnt/β-catenin signaling pathway(2022/01/01)
作者:Zheng Zhou, Tang Liu, Zhihong Li
期刊:Anti-Cancer Drugs
DOI:10.1097/CAD.0000000000001206
影响因子 :2.389