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产品概述
| 名称 | U-937 (人组织细胞淋巴瘤细胞) (STR鉴定正确) |
| 别称 | U937; U 937 |
| 种属 | 人 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 单核细胞 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 3×10^5-5×10^5cells/mL |
| 推荐换液频率 | 2~3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | U-937细胞是由Sundstrom和Nilsson于1974年建立，取材于患组织细胞淋巴瘤病人的胸水。研究表明：U-937细胞能被人混合淋巴细胞培养上清，佛波脂、维生素D3、γ干扰素、肿瘤坏死因子和维甲酸诱导终末单核细胞分化。U-937细胞免疫球蛋白产物和EB病毒表达为阴性；U-937细胞表达Fas抗原且对TNF和抗-Fas抗体敏感。 |
| 年龄（性别） | 男 |
| 组织来源 | 组织细胞淋巴瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~30-60 hours |
| 受体表达情况 | complement (C3) |
| 基因表达情况 | lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA) |
| 保藏机构 | ATCC; CRL-1593ATCC; CRL-1593.2 DSMZ; ACC-5 ECACC; 85011440 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,20 D3S1358 16 D5S818 12 D7S820 9,11 D8S1179 12,13 D13S317 10,12 D16S539 12 D18S51 13,14 D19S433 14,16 D21S11 27,29 FGA 22,25 PentaD 12,13 PentaE 13 TH01 6,9.3 TPOX 8,11 vWA 14,15 D6S1043 12,18 D12S391 17,18 D2S441 10 -
STR鉴定图
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参考文献
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An immune evasion mechanism with IgG4 playing an essential role in cancer and implication for immunotherapy(2020/08/20)
作者：Hui Wang, Qian Xu, Chanyuan Zhao
期刊：Journal for ImmunoTherapy of Cancer
影响因子 ：10.900
引用产品：CL-0239
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Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway(2022/10/18)
作者：Hanyue Li, Yu Yuan, Hongying Chen
期刊：Molecular Metabolism
DOI：10.1016/j.molmet.2022.101617
影响因子 ：8.100
引用产品：CL-0239
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Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation(2022/09/07)
作者：Peng Liu, Shuya Wang, Guangxin Wang
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 ：5.300
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miR‐129‐2‐3p directly targets SYK gene and associates with the risk of ischaemic stroke in a Chinese population(2018/11/29)
作者：Suli Huang, Ziquan Lv, Ying Wen
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.300
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MicroRNA-124a contributes to glucocorticoid resistance in acute-on-chronic liver failure by negatively regulating glucocorticoid receptor alpha(2019/10/01)
作者：Xin Wang, Hongrui Xu, Yadong Wang
期刊：Annals of Hepatology
DOI：10.1016/j.aohep.2019.08.007
影响因子 ：3.800
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MicroRNA-124a contributes to glucocorticoid resistance in acute-on-chronic liver failure by negatively regulating glucocorticoid receptor alpha(2019/10/1)
作者：Xin Wang, Hongrui Xu, Yadong Wang
期刊：Annals of Hepatology
DOI：10.1016/j.aohep.2019.08.007
影响因子 ：3.800
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Identification of Blood Let-7e-5p as a Biomarker for Ischemic Stroke(2016/10/24)
作者：Suli Huang, Ziquan Lv, Yi Guo
期刊：PLoS One
DOI：10.1371/journal.pone.0163951
影响因子 ：3.700
引用产品：CL-0239
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Matrisome provides a supportive microenvironment for oral squamous cell carcinoma progression(2021/12/16)
作者：Yungang He, Pingmeng Deng, Ying Yan
期刊：Journal of Proteomics
DOI：10.1016/j.jprot.2021.104454
影响因子 ：3.300
引用产品：CL-0239
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Human EVI2B acts as a Janus-faced oncogene/antioncogene by differently affecting as per cancer type neoplastic cells growth and immune infiltration:(2023/03/23)
作者：Fangyingnan Zhang, Xinning Wang, Xiaoqi Zhang
期刊：Oncologie
DOI：10.1515/oncologie-2022-1002
影响因子 ：0.900
