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产品概述
名称 | hFOB 1.19 (人SV40转染成骨细胞) (STR鉴定正确) (只出售冻存) |
别称 | hFOB1.19; hFOB |
背景描述 | hFOB 1.19细胞是在0.6mg/mL新霉素G418存在下,用温度敏感的表达载体pUCSVisA58转染自然流产的胎儿四肢组织建立的细胞系。在许可温度33.5℃下细胞分裂很快,而在限制温度39.5℃下细胞极少分裂或不分裂。hFOB 1.19细胞具有分化为表达造骨细胞表型的成熟造骨细胞的能力;hFOB 1.19细胞提供了一个同质化的快速增殖模型系统,用于研究正常人造骨细胞的分化、造骨细胞生理和激素、生长因子和对造骨细胞的功能及分化有影响的细胞因子。 |
种属 | 人 |
年龄(性别) | 胚胎 |
组织来源 | 骨;成骨细胞;以SV40巨细胞抗原转染 |
生长特性 | 贴壁细胞 |
细胞形态 | 多细胞形态 |
细胞类型 | 条件永生化细胞 |
生物安全等级 | 2 [Cells contain SV40 viral sequences] |
生长培养基 | DMEM/F12+0.3mg/ml G418+10% FBS+1% P/S |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:34℃ |
抗原表达情况 | SV40 T antigen |
基因表达情况 | alkaline phosphatase |
注意事项 | 该细胞培养难度较高;培养温度为度:33.5℃-34℃;培养基中添加G418。 |
保藏机构 | ATCC; CRL-11372 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,13 D2S1338 23,24 D3S1358 17,18 D5S818 11,12 D7S820 8,10 D8S1179 10,14 D13S317 11,12 D16S539 9,13 D18S51 10,17 D19S433 13,15 D21S11 29,32.2 FGA 19,22 PentaD 9,13 PentaE 8,11 TH01 7,9.3 TPOX 11 vWA 16,18 D6S1043 13,16 D12S391 20,23 D2S441 11,14 -
STR鉴定图
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参考文献
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The FAPα-activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway(2021/08/14)
作者:Geni Ye, Maohua Huang, Yong Li
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2021.08.015
影响因子 :14.903
引用产品:CL-0353
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Activation of cannabinoid receptor type 2-induced osteogenic differentiation involves autophagy induction and p62-mediated Nrf2 deactivation(2020/1/15)
作者:Xu Aihua, Yang Yang, Shao Yang
期刊:Cell Communication and Signaling
影响因子 :7.5250
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NPR3, transcriptionally regulated by POU2F1, inhibits osteosarcoma cell growth through blocking the PI3K/AKT pathway(2021/7/3)
作者:Shuo Li, Ruirong Guo, Zhibin Peng
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110074
影响因子 :4.8500
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma(2021/12/22)
作者:Bing Wang, Xiaowei Wang, Xing Tong
期刊:Frontiers in Genetics
影响因子 :4.772
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Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis(2019/1/2)
作者:Jiadong Wu, Aifei Wang, Xiao Wang
期刊:Bone
DOI:10.1016/j.bone.2018.12.019
影响因子 :4.6260
引用产品:CL-0353
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway(2021/09/13)
作者:Ke Li, Hongtao Yu, Chunbing Zhao
期刊:Journal of Cancer
影响因子 :4.478
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SRPX2 Promotes Cell Proliferation and Invasion in Osteosarcoma Through Regulating Hippo Signaling Pathway(2020/2/26)
作者:Zhiqiang Wu, Chunmeng Wang, Yong Chen
期刊:OncoTargets and Therapy
影响因子 :4.3450
引用产品:CL-0353
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Inhibiting effect of microRNA-187-3p on osteogenic differentiation of osteoblast precursor cells by suppressing cannabinoid receptor type 2(2019/7/13)
作者:Aihua Xu, Yang Yang, Yang Shao
期刊:Differentiation
DOI:10.1016/j.diff.2019.07.002. Epub 2019 Jul 13.
影响因子 :3.5330
引用产品:CL-0353
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Upregulation of miR‑95-3p inhibits growth of osteosarcoma by targeting HDGF(2019/6/10)
作者:Xiwei Liu, Wei Ma, Jianbing Ma
期刊:Pathology Research And Practice
影响因子 :3.3090
引用产品:CL-0353
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Identification of Candidate MicroRNA-mRNA Subnetwork for Predicting the Osteosarcoma Progression by Bioinformatics Analysis(2022/09/22)
作者:Dejie Lu, Hanji Huang, Li Zheng
期刊:Computational and Mathematical Methods in Medicine
影响因子 :2.8090
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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells(2022/11/08)
作者:Zhengjiang Li, Yan Zhang, Yongkui Li, Shuxing Xing, Shunqiang Li, Jing Lyu, Zhaonan Ban
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7510