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产品概述
名称 | Ca Ski (人宫颈癌肠转移细胞/人宫颈癌上皮细胞) (STR鉴定正确) |
别称 | Ca-Ski; CaSki; Caski; CASKI |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | RPMI-1640+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
背景描述 | Ca Ski细胞是从小肠肠系膜转移灶的细胞中建立的。据报道,Ca Ski细胞含有完整的HPV-16(每个细胞大约600个拷贝)和HPV-18相关序列。 |
年龄(性别) | 女;40岁 |
组织来源 | 宫颈癌肠转移 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 宫颈癌细胞 |
生物安全等级 | 2 |
倍增时间 | ~60-80 hours |
基因表达情况 | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
保藏机构 | ATCC; CRL-1550 ATCC; CRM-CRL-1550 ATCC; CRL-7915BCRC; 60251 ECACC; 87020501 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 21 D3S1358 15 D5S818 13 D7S820 8,11 D8S1179 15 D13S317 8,12 D16S539 11,12 D18S51 17 D19S433 15,16 D21S11 30 FGA 21 PentaD 10 PentaE 5 TH01 7 TPOX 8 vWA 17 D6S1043 11,14 D12S391 18 D2S441 11 -
STR鉴定图
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参考文献
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ASF1B promotes cervical cancer progression through stabilization of CDK9(2020/08/26)
作者:Xinjian Liu, Jingwei Song, Yenan Zhang
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02872-5
影响因子 :9.000
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Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway(2021/06/27)
作者:Qin Meng, Baofang Zhang, Yingming Zhang
期刊:Journal Of Cellular And Molecular Medicine
影响因子 :5.300
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KMT2A regulates cervical cancer cell growth through targeting VDAC1.(2020/05/21)
作者:Changlin Zhang, Yijun Hua, Huijuan Qiu
期刊:Aging-US
影响因子 :5.200
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CDK6 increases glycolysis and suppresses autophagy by mTORC1-HK2 pathway activation in cervical cancer cells(2022/02/15)
作者:Xiaoxi Zhang, Yunxia Sun, Siyao Cheng
期刊:Cell Cycle
DOI:10.1080/15384101.2022.2039981
影响因子 :5.173
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T-box transcription factor TBX1, targeted by microRNA-6727-5p, inhibits cell growth and enhances cisplatin chemosensitivity of cervical cancer cells through AKT and MAPK pathways(2021/2/8)
作者:Haixia Liu, Mei Song, Xiaoyan Sun
期刊:Bioengineered
DOI:10.1080/21655979.2021.1880732
影响因子 :4.900
引用产品:CL-0048
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OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway(2022/09/29)
作者:Limin Zhou, Hongying Li, Dunlan Zhang
期刊:ONCOLOGY REPORTS
影响因子 :4.200
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Vitamin E succinate exerts anti-tumour effects on human cervical cancer cells via the CD47-SIRPɑ pathway both in vivo and in vitro(2021/05/05)
作者:Xiaoli Huang, Markus Neckenig, Jintang Sun
期刊:Journal of Cancer
影响因子 :3.900
引用产品:CL-0048
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Characterization of the microenvironment in different immune-metabolism subtypes of cervical cancer with prognostic significance(2023/02/03)
作者:Wujiang Lai, Jinrong Liao, Xiaoxuan Li
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2023.1067666
影响因子 :3.700
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Signature of seven cuproptosis-related lncRNAs as a novel biomarker to predict prognosis and therapeutic response in cervical cancer.(2022/09/20)
作者:Xinyu Liu, Lei Zhou, Minghui Gao
期刊:Frontiers in Genetics
影响因子 :3.700
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MiRNA-211 triggers an autophagy-dependent apoptosis in cervical cancer cells: regulation of Bcl-2(2019/10/21)
作者:Shang Liu, Hongyan Wang, Jing Mu
期刊:Naunyn-Schmiedebergs Archives of Pharmacology
DOI:10.1007/s00210-019-01720-4
影响因子 :3.600
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Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells(2019/11/30)
作者:Rui Tong, Jingru Zhang, Chunyan Wang
期刊:Naunyn-Schmiedebergs Archives of Pharmacology
DOI:10.1007/s00210-019-01772-6
影响因子 :3.600
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Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells(2019/11/30)
作者:Rui Tong, Jingru Zhang, Chunyan Wang
期刊:Naunyn-Schmiedebergs Archives of Pharmacology
DOI:10.1007/s00210-019-01772-6
影响因子 :3.600
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hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR-217 to upregulate anterior gradient 2(2022/03/11)
作者:Yumei Lv, Mingyi Wang, Mingli Chen
期刊:Journal Of Clinical Laboratory Analysis
影响因子 :3.124
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tRNA‑derived fragment tRF‑Glu49 inhibits cell proliferation, migration and invasion in cervical cancer by targeting FGL1(2022/08/09)
作者:Yang Wang, Wenying Xia, Fangrong Shen
期刊:Oncology Letters
影响因子 :2.900
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Circ_0042986 Presence Restrains Cervical Cancer Development via Upregulating PEG3 by Directly Targeting miR-582-3p(2022/08/26)
作者:Jun Wang, Xing Chen, Lingzhi Zheng
期刊:Reproductive Sciences
DOI:10.1007/s43032-022-01053-3
影响因子 :2.900
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Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer(2023/09/16)
作者:Mei Wu, Mingji Ye
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01218-6
影响因子 :2.900
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Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer(2020/11/30)
作者:Guanglei Zhong, Xingyu Fang, Qingsheng Xie
期刊:Oncology Letters
影响因子 :2.900
引用产品:CL-0048
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SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A(2023/04/21)
作者:Boya Deng, Ailin Li, Ying Zhu
期刊:Oncology Letters
影响因子 :2.900
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Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway(2023/04/20)
作者:Zhimin Qi, Xia Wang, Xia Liu
期刊:Chinese Journal of Integrative Medicine
影响因子 :2.900
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FBXO39 predicts poor prognosis and correlates with tumor progression in cervical squamous cell carcinoma(2022/08/28)
作者:Yanru Yang, Yun Zhao, Guorui Sun
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma(2023/07/23)
作者:Yanru Yang, Saijie Zuo, Wenqing Li
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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ZIC5 promotes aggressiveness and cancer stemness in cervical squamous cell carcinoma(2022/12/05)
作者:Qingge Jia, Junyang Song, Tianqi Xu
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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Circular RNA circ_0000119 promotes cervical cancer cell growth and migration via miR-433-3p/PAK2 axis(2023/08/04)
作者:Junxiao Zhang, Guanghua Chu, Lihua Zheng
期刊:JOURNAL OF APPLIED GENETICS
DOI:10.1007/s13353-023-00772-w
影响因子 :2.400
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Circular RNA circVPRBP serves as a microRNA-106b-5p sponge to regulate proliferation and metastasis of cervical cancer cells via tripartite motif-containing protein 3(2022/08/10)
作者:Yingying Jiang, Wei Xu, Junzhen Tao
期刊:ANTI-CANCER DRUGS
DOI:10.1097/CAD.0000000000001335
影响因子 :2.300
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PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway(2023/01/05)
作者:Boya Deng, Siyang Zhang, Yingying Zhou
期刊:HISTOCHEMISTRY AND CELL BIOLOGY
DOI:10.1007/s00418-022-02175-0
影响因子 :2.300
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LncRNA Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) promotes the migration, invasion and epithelial-mesenchymal transition (EMT) through targeting miR-147a/insulin-like growth factor 1 receptor (IGF1R) pathway in cervical cancer tissues and c(2022/03/01)
作者:Limei Zhang, Yufei Cai, Chenchen Tian
期刊:Journal Of Obstetrics And Gynaecology Research
影响因子 :1.600