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产品概述
| 名称 | Caco-2 (人结直肠腺癌细胞) (STR鉴定正确) |
| 别称 | CaCo-2; CACO-2; Caco 2; CACO 2; CACO2; CaCo2; CaCO2; Caco2; Caco-2/ATCC |
| 背景描述 | Caco-2细胞分离自直肠原位癌；当长到满时，Caco-2细胞表现出特征性的肠上皮细胞分化。Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白Ⅱ，并呈角质蛋白阳性。 |
| 种属 | 人 |
| 年龄（性别） | 男；72岁 |
| 组织来源 | 结肠，结直肠腺癌 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | MEM（含NEAA）＋20% FBS＋1% P/S |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~60-80 hours |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 致瘤性 | Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II). |
| 受体表达情况 | This cell line expressed heat stable enterotoxin (Sta, E. coli) and epidermal growth factor (EGF). |
| 基因表达情况 | keratin, retinoic acid binding protein 1, retinol binding protein 2 |
| 注意事项 | 1. 细胞贴壁慢特别是刚复苏时，一般两到三天贴壁展开，会有空泡； 2. 细胞在传代细胞中注意消散，不然难贴壁； 3. 成团生长，细胞密度越大，表面空泡黑点越多； 4. 一般细胞长至80%传代，细胞成片，其实密度很大； 5. 细胞生长太满对细胞状态影响严重，传代后易碎，死亡； 6. 细胞生长时间越久，消化难度也会随之增加；消化到轻拍培养瓶侧边细胞可以滑落的时候可以终止。 |
| 保藏机构 | ATCC; HTB-37 BCRJ; 0059 DSMZ; ACC-169 ECACC; 09042001 ECACC; 86010202 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 17,25 D3S1358 14,17 D5S818 12,13 D7S820 11,12 D8S1179 12,14 D13S317 11,13,14 D16S539 12,13 D18S51 12 D19S433 15 D21S11 30,32 FGA 19 PentaD 9,11 PentaE 7 TH01 6 TPOX 9,11 vWA 16,18 D6S1043 12 D12S391 17,23 D2S441 10,15 -
STR鉴定图
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参考文献
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Oral delivery of decanoic acid conjugated plant protein shell incorporating hybrid nanosystem leverage intestinal absorption of polyphenols(2022/01/13)
作者：Ruimin Miao, Fanqi Jin, Zhigao Wang
期刊：Biomaterials
DOI：10.1016/j.biomaterials.2022.121373
影响因子 ：15.3040
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Ferroptosis-related Genes for Overall Survival Prediction in Patients with Colorectal Cancer can be Inhibited by Gallic acid(2021/03/01)
作者：Zongchao Hong, Peili Tang, Bo Liu
期刊：International Journal of Biological Sciences
影响因子 ：10.750
引用产品：CL-0050
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Soybean polysaccharide fermentation products regulate the air-liquid interface in co-cultured Caco-2 cells by increasing short chain fatty acids transport(2022/11/17)
作者：Li Li, Mengyu Li, Jingfan Wu, Qiuran Ji, Shengnan Wang, Hong Song, Ruren Li, Jun Liu, Lina Yang, He Liu
期刊：FOOD RESEARCH INTERNATIONAL
DOI：10.1016/j.foodres.2022.112136
影响因子 ：7.4250
引用产品：CL-0050
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Qing-Re-Xing-Yu decoction confers intestinal barrier protection by reducing epithelial cell pyroptosis in experimental ulcerative colitis based on a NLRP3-GSDMD axis-dependent mechanism(2021/08/21)
作者：Jing Yan, Tingting Cao, Ningyuan Ye
期刊：Phytomedicine
DOI：10.1016/j.phymed.2021.153725
影响因子 ：6.656
引用产品：CL-0050
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SEDDS facilitate cinnamaldehyde crossing the mucus barrier: The perspective of mucus and Caco-2/HT29 co-culture models(2022/01/10)
作者：Ye Cai, Liu Liu, Mengqiu Xia
期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2022.121461
影响因子 ：6.5100
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Paeoniflorin-6′-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB(2022/10/12)
作者：Mengya Jiang, Caihong Tang, Mei Yang
期刊：PHYTOTHERAPY RESEARCH
DOI：10.1002/ptr.7657
影响因子 ：6.3880
引用产品：CL-0050
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Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis(2022/10/27)
作者：Bo Cheng, Mengyu Du, Shuxuan He
期刊：MOLECULAR MEDICINE
DOI：10.1186/s10020-022-00556-8
影响因子 ：6.3760
引用产品：CL-0050
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Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders( 2021/12/30)
作者：Jingjing Liu, Wenyang Zhao, Chun Li
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：6.2080
引用产品：CL-0050
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Indole-3-propionic Acid Improved the Intestinal Barrier by Enhancing Epithelial Barrier and Mucus Barrier(2020/12/23)
作者：Jiaojiao Li, Li Zhang, Tao Wu
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：5.895
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Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway(2020/12/15)
作者：Bowen Zhu, Yanqin Wu, Lizhi Niu
期刊：Frontiers in Oncology
影响因子 ：5.738
引用产品：CL-0050
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影响因子 ：5.652
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Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin αVβ5 receptor(2019/11/07)
作者：Jianbin Bi, Jia Zhang, Yifan Ren
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.295
引用产品：CL-0050
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GABA increases susceptibility to DSS-induced colitis in mice(2022/11/12)
作者：Haojie Zhang, Yujin Wang, Fengyuan Gao, Rucheng Liu, Wen Chen, Xue Zhao, Qian Sun, Xiaotong Sun, Jingxin Li, Chuanyong Liu, Xuelian Ma
期刊：Journal of Functional Foods
影响因子 ：5.2230
引用产品：CL-0050
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Butyrate impeded the conscription of MDSCs to reduce CAC formation by blocking the TLR2 signaling pathway(2022/11/19)
作者：Tengfei Xiao, Mingzhong Sun, Yi Chang, Jingjing Kang, Chuanxiang Zhao, Rong Zhu, Hongmei Chen, Yetao Qiang
期刊：Journal of Functional Foods
影响因子 ：5.2230
引用产品：CL-0050
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Hydrogen sulfide improves colonic barrier integrity in DSS-induced inflammation in Caco-2 cells and mice(2016/7/18)
作者：Hongyu Zhao, Rui Yan, Xiaogang Zhou
期刊：International Immunopharmacology
DOI：10.1016/j.intimp.2016.07.020
影响因子 ：4.9320
引用产品：CL-0050
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Antioxidant Effects of Roasted Licorice in a Zebrafish Model and Its Mechanisms(2022/11/10)
作者：Qian Zhou, Shanshan Zhang, Xue Geng, Haiqiang Jiang, Yanpeng Dai, Ping Wang, Min Hua, Qi Gao, Shiyue Lang, Lijing Hou, Dianhua Shi, Meng Zhou
期刊：MOLECULES
影响因子 ：4.9270
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CCAAT/Enhancer-Binding Protein Homologous Protein (CHOP) Deficiency Attenuates Heatstroke-Induced Intestinal Injury(2021/11/29)
作者：Yan Cao, Maiying Fan, Yanfang Pei
期刊：Inflammation
DOI：10.1007/s10753-021-01577-x
影响因子 ：4.6570
引用产品：CL-0050
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Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1(2019/01/08)
作者：Jinliang Li, Yuxi Qi
期刊：Experimental and Molecular Pathology
DOI：10.1016/j.yexmp.2019.01.003
影响因子 ：4.401
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Human breast milk-derived exosomes may help maintain intestinal epithelial barrier integrity(2021/03/17)
作者：He Shan, Gang Liu, Xueping Zhu
期刊：Pediatric Research
DOI：10.1038/s41390-021-01449-y
影响因子 ：3.953
引用产品：CL-0050
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Adsorption Behavior of Nonylphenol on Polystyrene Microplastics and Their Cytotoxicity in Human Caco-2 Cells(2022/10/18)
作者：Fangfang Ding, Qianqian Zhao, Luchen Wang
期刊：Water
影响因子 ：3.5300
引用产品：CL-0050
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Targeting proteasome enhances anticancer activity of oncolytic HSV-1 in colorectal cancer(2022/11/11)
作者：Xiaxi Li, Wei Hu, Jiangang Shen, Mingsong Li, Wei Gong
期刊：VIROLOGY
DOI：10.1016/j.virol.2022.11.002
影响因子 ：3.5130
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis(2021/8/10)
作者：HaiLin Xiong, XiaoHua Zhong, XiaoHong Guo
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.7510
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Intestinal ischemia‑reperfusion induces the release of IL‑17A to regulate cell inflammation, apoptosis and barrier damage(2021/12/17)
作者：Li Xiao, Wanhua Zhang, Yin Huang
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.7510
引用产品：CL-0050
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Genistein improves inflammatory response and colonic function through NF-κB signal in DSS-induced colonic injury(2017/05/26)
作者：Rui Zhang, Jian Xu, Jian Zhao
期刊：Oncotarget
影响因子 ：0.0000
引用产品：CL-0050
