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产品概述
| 名称 | Caki-1 (人肾透明细胞癌皮肤转移细胞) (STR鉴定正确) |
| 别称 | CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | McCoy's 5A＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | Caki-1细胞的超微结构中包含许多微绒毛、少许微丝、许多小线粒体、充分发育的高尔基体、内质网、许多脂滴和多层体，次级溶酶体；目前，没有在Caki-1细胞内发现病毒颗粒。 |
| 年龄（性别） | 男；49岁 |
| 组织来源 | 肾透明细胞癌皮肤转移 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肾癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~40-60 hours |
| 致瘤性 | Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters. |
| 抗原表达情况 | Blood Type O; Rh-; HLA A9, B12, Bw35 |
| 保藏机构 | ATCC; HTB-46 DSMZ; ACC-142DSMZ; ACC-731 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11 D2S1338 17,19 D3S1358 17 D5S818 11,12 D7S820 8,12 D8S1179 12,14 D13S317 11,12 D16S539 12 D18S51 14 D19S433 14 D21S11 28,30 FGA 26 PentaD 11,12 PentaE 11,22 TH01 6,8 TPOX 8,11 vWA 15,17 D6S1043 11,12 D12S391 15,17 D2S441 12,14 -
STR鉴定图
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参考文献
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Tumor promoting effects of circRNA_001287 on renal cell carcinoma through miR-144-targeted CEP55(2020/12/01)
作者：Jiafu Feng, Yongcan Guo, Yuanmeng Li
期刊：Journal Of Experimental & Clinical Cancer Research
DOI：10.1186/s13046-020-01744-2
影响因子 ：11.300
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Serine hydroxymethyltransferase 2 knockdown induces apoptosis in ccRCC by causing lysosomal membrane permeabilization via metabolic reprogramming(2023/02/20)
作者：Zhangnan Liu, Mengzhen Fan, Junqing Hou
期刊：Cell Death & Disease
DOI：10.1038/s41419-023-05677-4
影响因子 ：9.000
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Identification of the H3K36me3 reader LEDGF/p75 in the pancancer landscape and functional exploration in clear cell renal cell carcinoma(2023/08/26)
作者：Yuwei Zhang, Wei Guo, Yangkun Feng
期刊：Computational and Structural Biotechnology Journal
DOI：10.1016/j.csbj.2023.08.023
影响因子 ：6.000
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LncRNA-LET inhibits cell growth of clear cell renal cell carcinoma by regulating miR-373-3p(2019/11/21)
作者：Zhuo Ye, Jiachen Duan, Lihui Wang
期刊：Cancer Cell International
影响因子 ：5.800
引用产品：CL-0052
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Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.(2022/09/21)
作者：Longfei Yang, Yuwei Zhang, Yang Wang
期刊：Frontiers in Pharmacology
影响因子 ：5.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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LncRNA-encoded Micropeptide ACLY-BP drives lipid deposition and cell proliferation in Clear Cell Renal Cell Carcinoma via maintenance of ACLY acetylation(2023/07/06)
作者：Shenghua Zhang, Zheng Zhang, Xiaolong Liu
期刊：MOLECULAR CANCER RESEARCH
DOI：10.1158/1541-7786.MCR-22-0781
影响因子 ：5.200
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Spindle pole body component 24 homolog potentiates tumor progression via regulation of SRY-box transcription factor 2 in clear cell renal cell carcinoma(2022/01/14)
作者：Chengfang Sun, Jingchao Wei, Zhilin Long
期刊：Faseb Journal
影响因子 ：4.800
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Identification of the regulatory mechanism of ACE2 in COVID-19–induced kidney damage with systems genetics approach(2023/03/23)
作者：Xueling Yang, Chunhua Lin, Jian Liu
期刊：JOURNAL OF MOLECULAR MEDICINE-JMM
DOI：10.1007/s00109-023-02304-9
影响因子 ：4.700
引用产品：CL-0052
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A pan-cancer analysis of DDR1 in prognostic signature and tumor immunity, drug resistance(2023/04/08)
作者：Longfei Yang, Yuwei Zhang, Yifan Tang
期刊：Scientific Reports
DOI：10.1038/s41598-023-27975-9
影响因子 ：4.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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HOXD1 functions as a novel tumor suppressor in kidney renal clear cell carcinoma(2021/2/19)
作者：Yuanbo Cui, Chunyan Zhang, Ya Li
期刊：Cell Biology International
影响因子 ：3.900
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The potential of Lycium barbarum miR166a in kidney cancer treatment(2022/12/28)
作者：Qiang Zhang, Zhiyuan Xie, Yan Li
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2022.113455
影响因子 ：3.700
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Comprehensive analysis of the HOXA gene family identifes HOXA13as a novel oncogenic gene in kidney renal clear cell carcinoma(2020/05/22)
作者：Yuanbo Cui, Ming Yan, Chunyan Zhang
期刊：JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI：10.1007/s00432-020-03259-x
影响因子 ：3.600
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P4HA3 promotes clear cell renal cell carcinoma progression via the PI3K/AKT/GSK3β pathway(2023/01/02)
作者：Zhechuan Zhang, Yuanfeng Zhang, Ronggui Zhang
期刊：MEDICAL ONCOLOGY
DOI：10.1007/s12032-022-01926-2
影响因子 ：3.400
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Sertraline/ICG‐loaded liposome for dual‐modality imaging and effective chemo‐photothermal combination therapy against metastatic clear cell renal cell carcinoma(2020/1/14)
作者：Yuting Lei, Li Zeng, Shuqing Xie
期刊：Chemical Biology & Drug Design
影响因子 ：3.000
引用产品：CL-0052
