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| 名称 | HT-29 (人结肠癌细胞) (STR鉴定正确) |
| 别称 | HT 29; HT29 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | McCoy’s 5A＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的；近来，已建株的培养细胞用含相同血清McCoy's5A培液培养。HT-29细胞在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
| 年龄（性别） | 女；44岁 |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~36-60 hours |
| 致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
| 受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
| 基因表达情况 | secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). |
| 保藏机构 | ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 19,23 D3S1358 15,17 D5S818 11,12 D7S820 10 D8S1179 10,16 D13S317 11,12 D16S539 11,12 D18S51 13 D19S433 14 D21S11 29,30 FGA 20,22 PentaD 11,13 PentaE 14,16 TH01 6,9 TPOX 8,9 vWA 17,19 D6S1043 12，14 D12S391 18.3,21 D2S441 11 -
STR鉴定图
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参考文献
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SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip(2020/12/01)
作者：Yaqiong Guo, Ronghua Luo, Yaqing Wang
期刊：Science Bulletin
DOI：10.1016/j.scib.2020.11.015
影响因子 ：18.900
引用产品：CL-0118
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Bacteria-Induced Colloidal Encapsulation for Probiotic Oral Delivery(2023/03/22)
作者：Chong Zhang, Xiaorong Gao, Xinxiu Ren
期刊：ACS Nano
影响因子 ：17.100
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Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer(2022/08/28)
作者：Meng Tang, Hui Xu, Hongyan Huang
期刊：Advanced Science
影响因子 ：15.100
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An enhanced cascade-based deep forest model for drug combination prediction(2022/03/10)
作者：Weiping Lin, Lianlian Wu, Yixin Zhang
期刊：Briefings In Bioinformatics
影响因子 ：13.994
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Nitroreductase-instructed supramolecular assemblies for microbiome regulation to enhance colorectal cancer treatments(2022/11/09)
作者：Jiali Chen, Pai Zhang, Yan Zhao, Jie Zhao, Xiaobo Wu, Ruijia Zhang, Ruitao Cha, Qingxin Yao, Yuan Gao
期刊：Science Advances
影响因子 ：13.600
引用产品：CL-0071 CL-0096 CL-0101 CL-0118 PM150110 PM150210 PM150710
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022/12/15)
作者：Chi Jin, Tuo Wang, Dongsheng Zhang
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影响因子 ：11.300
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The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation(2021/10/26)
作者：Yan Li, Na Zhang, Chao Ma
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-04289-0
影响因子 ：9.000
引用产品：CL-0118
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A possible but unrecognized risk of acceptable daily intake dose triazole pesticides exposure—bile acid disturbance induced pharmacokinetic changes of oral medication(2023/02/21)
作者：Yujing Xu, Yufeng Zhang, Quan Tao
期刊：CHEMOSPHERE
DOI：10.1016/j.chemosphere.2023.138209
影响因子 ：8.800
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Anti-colon cancer effects of Spirulina polysaccharide and its mechanism based on 3D models(2022/12/26)
作者：Yingfang Lu, Zexin Chen, Qianmin Lin
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2022.12.244
影响因子 ：8.200
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Targeted EpCAM-binding for the development of potent and effective anticancer proteins(2023/02/28)
作者：Zhao Liu, Chen Zhang, Beiming Cui
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114443
影响因子 ：7.500
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Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy(2021/07/20)
作者：Dandan Wang, Shuai Shao, Yanqiu Zhang
期刊：Frontiers in Immunology
影响因子 ：7.300
引用产品：CL-0118
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Indole-3-propionic Acid Improved the Intestinal Barrier by Enhancing Epithelial Barrier and Mucus Barrier(2020/12/23)
作者：Jiaojiao Li, Li Zhang, Tao Wu
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.100
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SEDDS facilitate cinnamaldehyde crossing the mucus barrier: The perspective of mucus and Caco-2/HT29 co-culture models(2022/01/10)
作者：Ye Cai, Liu Liu, Mengqiu Xia
期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2022.121461
影响因子 ：5.800
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A Genetic Algorithm-Based Ensemble Learning Framework for Drug Combination Prediction(2023/06/11)
作者：Lianlian Wu, Xiaona Ye, Yixin Zhang
期刊：Journal of Chemical Information and Modeling
影响因子 ：5.600
引用产品：CL-0118
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Palmitic acid-induced ferroptosis via CD36 activates ER stress to break calcium-iron balance in colon cancer cells(2023/03/12)
作者：Hao Kuang, Xuehua Sun, Ying Liu
期刊：FEBS Journal
影响因子 ：5.400
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Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer(2023/06/05)
作者：Qian Kang, Xin Guo, Tianfu Li
期刊：Frontiers in Microbiology
DOI：10.3389/fmicb.2023.1192900
影响因子 ：5.200
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MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2(2021/10/16)
作者：Bo Li, Xianyi Liu, Guogang Wu
期刊：Bioengineered
DOI：10.1080/21655979.2021.1996505.
影响因子 ：4.900
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Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition(2020/07/10)
作者：Yan Lin, Moussa Harouna Koumba, Suxuan Qu
期刊：Cellular Signalling
DOI：10.1016/j.cellsig.2020.109707
影响因子 ：4.800
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Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis(2022/11/21)
作者：Shanshan Xu, Youran Li, Hua Huang, Xian Miao, Yunfei Gu
期刊：Journal of Oncology
影响因子 ：4.501
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Oct4 promoted proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in colon cancer cells by activating the SCF/c-Kit signaling pathway(2022/10/18)
作者：Xiaoqian Bu, Yulong Liu, Lin Wang
期刊：CELL CYCLE
DOI：10.1080/15384101.2022.2112486
影响因子 ：4.300
引用产品：CL-0118
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Label-free classification of dead and live colonic adenocarcinoma cells based on 2D light scattering and deep learning analysis(2021/6/19)
作者：Shuaiyi Li, Ya Li, Jianning Yao
期刊：Cytometry Part A
影响因子 ：3.700
引用产品：CL-0118
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P300 increases CSNK2A1 expression which accelerates colorectal cancer progression through activation of the PI3K-AKT-mTOR axis(2023/06/28)
作者：Jilong Liu
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2023.113694
影响因子 ：3.700
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者：Linping Lei, Xuqin Xie, Long He
期刊：Investigational New Drugs
DOI：10.1007/s10637-020-01014-0
影响因子 ：3.400
引用产品：CL-0096 CL-0118 CL-0144 CL-0196 CL-0223A CL-0223B CL-0225A CL-0225B
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Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model(2021/2/17)
作者：Qinghui Jia, Haihua Zhang, Yongmei Su
期刊：Journal Of Physiology And Biochemistry
DOI：10.1007/s13105-021-00796-y
影响因子 ：3.400
引用产品：CL-0118
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Procyanidin A1 alleviates DSS-induced ulcerative colitis via regulating AMPK/mTOR/p70S6K-mediated autophagy(2022/01/10)
作者：Haihua Zhang , Wuying Lang , Xin Liu
期刊：Journal Of Physiology And Biochemistry
DOI：10.1007/s13105-021-00854-5
影响因子 ：3.400
引用产品：CL-0118
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CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway(2022/08/15)
作者：Xiaolong Zhang, Lifei Tian, Zeyu Li
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.08.036
影响因子 ：3.100
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Osthole induces apoptosis of the HT-29 cells via endoplasmic reticulum stress and autophagy(2021/8/10)
作者：Xinghua Zhou, Jian Kang, Zhendong Zhong
期刊：Oncology Letters
影响因子 ：2.900
引用产品：CL-0118
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Tanshinone IIA May Inhibit Gastric Cancer via Affecting the Intestinal Microbiome(2022/09/26)
作者：Fenggang Lu, Yi Zhang, Pinghui Song
期刊：Computational and Mathematical Methods in Medicine
影响因子 ：2.809
引用产品：CL-0118
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Anti-cancer effects of Porphyra haitanensis polysaccharides on human colon cancer cells via cell cycle arrest and apoptosis without causing adverse effects in vitro(2020/8/11)
作者：WanZi Yao, Suresh Veeraperumal, HuaMai Qiu
期刊：3 BIOTECH
DOI：10.1007/s13205-020-02379-y
影响因子 ：2.800
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis(2021/8/10)
作者：HaiLin Xiong, XiaoHua Zhong, XiaoHong Guo
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
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ISLR affects colon cancer progression by regulating the epithelial–mesenchymal transition signaling pathway(2022/01/01)
作者：Chunhua Chi, Tongming Liu, Shengnan Yang
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001233
影响因子 ：2.300
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The Upregulation of GSTO2 is Associated with Colon Cancer Progression and a Poor Prognosis(2023/01/11)
作者：Xingyu Peng, Jinfeng Zhu, Sicheng Liu
期刊：Journal of Oncology
影响因子 ：0.000
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Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics(2023/01/05)
作者：Tingting Li, Hang Li, Wenxin Xia
期刊：Oxidative Medicine and Cellular Longevity
影响因子 ：0.000
