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产品概述
名称 | HT-29 (人结肠癌细胞) (STR鉴定正确) |
别称 | HT 29; HT29 |
种属 | 人类 |
年龄(性别) | 女性,44岁 |
组织来源 | 结直肠腺癌 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的;近来,已建株的培养细胞用含相同血清McCoy's5A培液培养。HT-29细胞在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
生物安全等级 | 1 |
生长培养基 | McCoy’s 5A+10% FBS+1% P/S |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~36-60小时 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
基因表达情况 | secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). |
保藏机构 | ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201 |
STR鉴定
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STR位点信息
Amelogenin X,X CSF1PO 11,12 D2S1338 19,23 D3S1358 15,17 D5S818 11,12 D7S820 10,10 D8S1179 10,16 D13S317 11,12 D16S539 11,12 D18S51 13,13 D19S433 14,14 D21S11 29,30 FGA 20,22 PentaD 11,13 PentaE 14,16 TH01 6,9 TPOX 8,9 vWA 17,19 D6S1043 12,14 D12S391 18.3,21 D2S441 11,11 -
STR鉴定图
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参考文献
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SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip(2020/12/1)
作者:Yaqiong Guo, Ronghua Luo, Yaqing Wang
期刊:Sci Bull (Beijing)
DOI:10.1016/j.scib.2020.11.015
影响因子 :11.78
引用产品:CL-0118
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The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation(44495)
作者:Li, Yan, Zhang, Na, Ma, Chao
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-04289-0
影响因子 :8.469
引用产品:CL-0118
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Insight Into Polysaccharides From Panax ginseng C. A. Meyer in Improving Intestinal Inflammation: Modulating Intestinal Microbiota and Autophagy(2021/07/20)
作者:Dandan Wang, Shuai Shao, Yanqiu Zhang
期刊:Frontiers in Immunology
影响因子 :7.561
引用产品:CL-0118
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Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition(2020.07.10)
作者:Yan Lin, Moussa Harouna Koumba, Suxuan Qu
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2020.109707
影响因子 :4.315