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产品概述
| 名称 | NCI-H520 (人肺鳞癌细胞) (STR鉴定正确) |
| 别称 | H520; H-520; NCI-HUT-520; NCIH520 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | NCI-H520细胞是由Gazdar AF等在1982年建系而来，源于鳞状细胞肺癌组织；NCI-H520细胞p53 mRNA表达水平较正常肺组织低，但是没有大的结构DNA的异常。NCI-H520细胞角蛋白、波形蛋白阳性，神经丝三联蛋白阴性；NCI-H520细胞在软琼脂中可形成克隆。 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~32-60 hours |
| 致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
| 保藏机构 | ATCC; HTB-182 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 12,13 D7S820 8,12 D8S1179 14,16,17 D13S317 10,11 D16S539 8,13 D18S51 17 D19S433 13,14 D21S11 30 FGA 22 PentaD 13 PentaE 5,14 TH01 10 TPOX 8 vWA 18,19 D6S1043 12,18 D12S391 21 D2S441 10,11 -
STR鉴定图
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参考文献
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Tambulin Targets Histone Deacetylase 1 Inhibiting Cell Growth and Inducing Apoptosis in Human Lung Squamous Cell Carcinoma(2020/08/12)
作者：Wuming Wang, Yuzhen Liu, Long Zhao
期刊：Frontiers in Pharmacology
影响因子 ：5.600
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Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway(2021/12/07)
作者：Zhonghai Xie, Hongwei Li, Jin Zang
期刊：Bioengineered
DOI：10.1080/21655979.2021.2005931
影响因子 ：4.900
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Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1(2022/02/17)
作者：Hongyang Shi, Yonghong Zhang, Yu Wang
期刊：Toxicology And Applied Pharmacology
DOI：10.1016/j.taap.2022.115926
影响因子 ：4.460
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TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT(2021/11/7)
作者：Danjie Zhang, Liangzhang Sun, Jin Zhang
期刊：Toxicology And Applied Pharmacology
DOI：10.1016/j.taap.2021.115793
影响因子 ：3.800
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SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway(2020/10/27)
作者：Yan Feng, Xiangyu Guo, Huaping Tang
期刊：Annals of Translational Medicine
影响因子 ：3.616
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Resveratrol improves the cytotoxic effect of CD8 +T cells in the tumor microenvironment by regulating HMMR/Ferroptosis in lung squamous cell carcinoma(2023/03/15)
作者：Shan Gao, Minchao Kang, Jizhao Wang
期刊：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
DOI：10.1016/j.jpba.2023.115346
影响因子 ：3.400
引用产品：CL-0402
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Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells(2021/7/1)
作者：Chenglin Yang, Shiping Zhu, Weifeng Feng
期刊：Oncology Letters
影响因子 ：2.900
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Palbociclib Induces the Apoptosis of Lung Squamous Cell Carcinoma Cells via RB-Independent STAT3 Phosphorylation(2022/08/18)
作者：Wenjing Xiang, Wanchen Qi, Huayu Li
期刊：Current Oncology
影响因子 ：2.600
引用产品：CL-0402
