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![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0145-1.jpg)
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![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0145-3.jpg)
![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0145-4.jpg)
![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0145-5.jpg)
![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0145-6.jpg)
![LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-1.jpg)
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产品概述
产品信息
| 名称 | LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确) |
| 别称 | Ls174T; LS174t; Ls-174-T; LS-174-T; LS 174 T; LS-174T; Ls-174T; LS 174T; LS-174; LS174 |
| 种属 | 人 |
| 年龄（性别） | 女；58岁 |
| 组织来源 | 直肠；结直肠腺癌 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 背景描述 | LS 174T细胞是LS 180细胞的胰蛋白酶化变种。LS 174T细胞比亲本更易传代，像LS 180细胞一样生成大量的癌胚抗原(CEA)。电镜研究表明，LS 174T细胞有丰富的微丝和细胞质粘液素液泡。LS 174T细胞直肠抗原3阳性；p53抗原表达阴性，但mRNA表达阳性。LS 174T细胞角蛋白染色阳性；LS 174T细胞癌基因c-myc、N-myc、H-ras、N-ras、Myb和fos的表达呈阳性；癌基因k-ras和sis的表达未做检测。 |
| 生物安全等级 | 1 |
| 生长培养基 | MEM(PM150467)＋10% FBS(164210-500)＋1% P/S(PB180120) |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~30-40 hours |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表达情况 | serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood Type O |
| 基因表达情况 | carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin The production of CEA in the ATCC seed stock was 1944 ng per 10^6 cells in 10 days. |
| 保藏机构 | ATCC; CL-188 ATCC; CCL-188 BCRC; 60053 DSMZ; ACC-759 ECACC; 87060401 |
