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产品概述
产品信息
| 名称 | LS 174T [LS174T] (人结肠腺癌细胞) (STR鉴定正确) |
| 别称 | Ls174T; LS174t; Ls-174-T; LS-174-T; LS 174 T; LS-174T; Ls-174T; LS 174T; LS-174; LS174 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | MEM（ATCC改良）＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
参考资料(来源文献)
| 背景描述 | LS 174T细胞是LS 180细胞的胰蛋白酶化变种。LS 174T细胞比亲本更易传代，像LS 180细胞一样生成大量的癌胚抗原(CEA)。电镜研究表明，LS 174T细胞有丰富的微丝和细胞质粘液素液泡。LS 174T细胞直肠抗原3阳性；p53抗原表达阴性，但mRNA表达阳性。LS 174T细胞角蛋白染色阳性；LS 174T细胞癌基因c-myc、N-myc、H-ras、N-ras、Myb和fos的表达呈阳性；癌基因k-ras和sis的表达未做检测。 |
| 年龄（性别） | 女；58岁 |
| 组织来源 | 直肠；结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~30-40 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表达情况 | serologically defined colon cancer antigen 3; Homo sapiens, expressedHLA A2, B13, B50; Blood Type O |
| 基因表达情况 | carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin The production of CEA in the ATCC seed stock was 1944 ng per 10^6 cells in 10 days. |
| 保藏机构 | ATCC; CL-188 ATCC; CCL-188BCRC; 60053 DSMZ; ACC-759 ECACC; 87060401 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,14 D2S1338 18,22 D3S1358 15,17 D5S818 11,15 D7S820 10.3,11 D8S1179 12,16 D13S317 10 D16S539 11,13 D18S51 11,13 D19S433 14,15 D21S11 29,31 FGA 21,22 PentaD 10 PentaE 15,16 TH01 6,7 TPOX 8,9 vWA 15,17 D6S1043 12,13 D12S391 18,20 D2S441 10,11 -
STR鉴定图
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参考文献
文献列表
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Nested Phosphorothioated Hybrid Primer-Mediated Isothermal Amplification for Specific and Dye-Based Subattomolar Nucleic Acid Detection at Low Temperatures(2023/03/03)
作者：Yaru Wang, Lanxiang Zhang, Yuqing Shen
期刊：ACS Sensors
DOI：10.1021/acssensors.2c02754
影响因子 ：8.900
引用产品：CL-0145
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The Milk Active Ingredient, 2′-Fucosyllactose, Inhibits Inflammation and Promotes MUC2 Secretion in LS174T Goblet Cells In Vitro(2023/01/01)
作者：Qianqian Yao, Huiying Li, Yanan Gao
期刊：Foods
影响因子 ：5.200
引用产品：CL-0145
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