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产品概述
| 名称 | MCF7 [MCF-7] (人乳腺癌细胞) (STR鉴定正确) |
| 别称 | MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL |
| 种属 | 人类 |
| 年龄（性别） | 女性，69岁 |
| 组织来源 | 乳腺，乳房；源自转移部位：胸腔积液 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 背景描述 | MCF7 [MCF-7]细胞保留了多个分化了的乳腺上皮的特性，包括：能通过胞质雌激素受体加工雌二醇并能形成圆形复合物。MCF7 [MCF-7]细胞含有Tx-4癌基因；肿瘤坏死因子α可以抑制MCF7 [MCF-7]细胞的生长，抗雌激素处理MCF7 [MCF-7]细胞能调变IGFBP'S的分泌。 |
| 生物安全等级 | 1 |
| 生长培养基 | MEM(PM150410)＋0.01mg/ml Insulin＋10% FBS(164210-500)＋1% P/S(PB180120) |
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~30-72小时 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 受体表达情况 | estrogen receptor, expressed |
| 抗原表达情况 | Blood Type O; Rh+ |
| 基因表达情况 | insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5 |
| 保藏机构 | ATCC; CRL-12584 ATCC; HTB-22 BCRC; 60436 BCRJ; 0162 DSMZ; ACC-115 ECACC; 86012803 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 21,23 D3S1358 16 D5S818 11,12 D7S820 8,9 D8S1179 10,14 D13S317 11 D16S539 11,12 D18S51 14 D19S433 13,14 D21S11 30 FGA 23,25 PentaD 12 PentaE 7,12 TH01 6,6 TPOX 9,12 vWA 14,15 D6S1043 12,18 D12S391 18,20 D2S441 10,14 -
STR鉴定图
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参考文献
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SO2 prodrug doped nanorattles with extra-high drug payload for “collusion inside and outside” photothermal/pH triggered - gas therapy(2020.10)
作者：Qianglan Lu, TongLu, Min Xu
期刊：Biomaterials
DOI：10.1016/j.biomaterials.2020.120236
影响因子 ：10.317
引用产品：CL-0149
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Photoelectrochemical cell enhanced by ternary heterostructured photoanode: Toward high-performance self-powered cathodic cytosensing(2019.07.15)
作者：Gao-Chao Fan, YanweiLu, HuanZhao
期刊：Biosensors and Bioelectronics
DOI：10.1016/j.bios.2019.04.063
影响因子 ：10.257
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Target-induced formation of multiple DNAzymes in solid-state nanochannels: Toward innovative photoelectrochemical probing of telomerase activity(2019.10.01)
作者：Gao-Chao Fan, Yanwei Lu, Linzheng Ma
期刊：Biosensors and Bioelectronics
DOI：10.1016/j.bios.2019.111564
影响因子 ：10.257
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Phenotype-related drug sensitivity analysis of single CTCs for medicine evaluation(2020.08.10)
作者：Haimeng Pei, Mei Yu, Defang Dong, Yiguo Wang
期刊：Chem. Sci.
影响因子 ：9.346
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Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device(2019.08.02)
作者：Haimeng Pei, Lu Li
期刊：Anal. Chem
DOI：10.1021/acs.analchem.9b01647
影响因子 ：6.785
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A gold–selenium-bonded nanoprobe for real-time in situ imaging of the upstream and downstream relationship between uPA and MMP-9 in cancer cells(2019.03.16)
作者：Mingming Luan
期刊：Chem Commun (Camb)
影响因子 ：6.12
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Low Dose of Paclitaxel Combined with XAV939 Attenuates Metastasis, Angiogenesis and Growth in Breast Cancer by Suppressing Wnt Signaling(14 August 2019)
作者：Dattatrya Shetti, Bao Zhang
期刊：Cells
影响因子 ：4.366
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A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation(2019.09.21)
作者：Peng Zhang, Shasha Li, Caixia Fu, Qian Zhang
期刊：Analyst
影响因子 ：3.978
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Possible involvement of TGF‑β‑SMAD‑mediated epithelial‑mesenchymal transition in pro‑metastatic property of PAX6(2020.06.11)
作者：Meng Jin, Daili Gao, Rongchun Wang
期刊：Oncology Reports
影响因子 ：3.417
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Effect of pluronic block composition on the structure, stability, and cytotoxicity of liposomes(2020.01.20)
作者：Ziling Li
期刊：Journal of Dispersion Science and Technology
DOI：10.1080/01932691.2020.1776130
影响因子 ：1.701
引用产品：CL-0149
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