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产品概述
名称 | MCF7 [MCF-7] (人乳腺癌细胞) (STR鉴定正确) |
别称 | MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL |
种属 | 人 |
年龄(性别) | 女;69岁 |
组织来源 | 乳腺,乳房;源自转移部位:胸腔积液 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | MCF7 [MCF-7]细胞保留了多个分化了的乳腺上皮的特性,包括:能通过胞质雌激素受体加工雌二醇并能形成圆形复合物。MCF7 [MCF-7]细胞含有Tx-4癌基因;肿瘤坏死因子α可以抑制MCF7 [MCF-7]细胞的生长,抗雌激素处理MCF7 [MCF-7]细胞能调变IGFBP'S的分泌。 |
生物安全等级 | 1 |
生长培养基 | MEM(PM150410)+0.01mg/ml Insulin+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~30-72 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | estrogen receptor, expressed |
抗原表达情况 | Blood Type O; Rh+ |
基因表达情况 | insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5 |
保藏机构 | ATCC; CRL-12584 ATCC; HTB-22 BCRC; 60436 BCRJ; 0162 DSMZ; ACC-115 ECACC; 86012803 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 21,23 D3S1358 16 D5S818 11,12 D7S820 8,9 D8S1179 10,14 D13S317 11 D16S539 11,12 D18S51 14 D19S433 13 D21S11 30 FGA 23,25 PentaD 12 PentaE 7,12 TH01 6 TPOX 9,12 vWA 14,15 D1S1656 11,15.3 D6S1043 12,18 D12S391 18,20 -
STR鉴定图
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参考文献
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SO2 prodrug doped nanorattles with extra-high drug payload for “collusion inside and outside” photothermal/pH triggered - gas therapy(2020.10)
作者:Qianglan Lu, TongLu, Min Xu
期刊:Biomaterials
DOI:10.1016/j.biomaterials.2020.120236
影响因子 :10.317
引用产品:CL-0149
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Photoelectrochemical cell enhanced by ternary heterostructured photoanode: Toward high-performance self-powered cathodic cytosensing(2019.07.15)
作者:Gao-Chao Fan, YanweiLu, HuanZhao
期刊:Biosensors and Bioelectronics
DOI:10.1016/j.bios.2019.04.063
影响因子 :10.257
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Target-induced formation of multiple DNAzymes in solid-state nanochannels: Toward innovative photoelectrochemical probing of telomerase activity(2019.10.01)
作者:Gao-Chao Fan, Yanwei Lu, Linzheng Ma
期刊:Biosensors and Bioelectronics
DOI:10.1016/j.bios.2019.111564
影响因子 :10.257
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Phenotype-related drug sensitivity analysis of single CTCs for medicine evaluation(2020.08.10)
作者:Haimeng Pei, Mei Yu, Defang Dong, Yiguo Wang
期刊:Chem. Sci.
影响因子 :9.346
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Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device(2019.08.02)
作者:Haimeng Pei, Lu Li
期刊:Anal. Chem
DOI:10.1021/acs.analchem.9b01647
影响因子 :6.785
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A gold–selenium-bonded nanoprobe for real-time in situ imaging of the upstream and downstream relationship between uPA and MMP-9 in cancer cells(2019.03.16)
作者:Mingming Luan
期刊:Chem Commun (Camb)
影响因子 :6.12
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Low Dose of Paclitaxel Combined with XAV939 Attenuates Metastasis, Angiogenesis and Growth in Breast Cancer by Suppressing Wnt Signaling(14 August 2019)
作者:Dattatrya Shetti, Bao Zhang
期刊:Cells
影响因子 :4.366
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A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation(2019.09.21)
作者:Peng Zhang, Shasha Li, Caixia Fu, Qian Zhang
期刊:Analyst
影响因子 :3.978
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Possible involvement of TGF‑β‑SMAD‑mediated epithelial‑mesenchymal transition in pro‑metastatic property of PAX6(2020.06.11)
作者:Meng Jin, Daili Gao, Rongchun Wang
期刊:Oncology Reports
影响因子 :3.417
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Effect of pluronic block composition on the structure, stability, and cytotoxicity of liposomes(2020.01.20)
作者:Ziling Li
期刊:Journal of Dispersion Science and Technology
DOI:10.1080/01932691.2020.1776130
影响因子 :1.701
引用产品:CL-0149