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产品概述
| 名称 | TM3 (小鼠睾丸间质细胞) (STR鉴定正确) |
| 别称 | TM-3 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | TM3细胞在LH作用下cAMP产量升高，但对促卵泡激素没有响应。TM3细胞对LH的响应持续时间与血清批次有关；在LH存在下，TM3细胞可以代谢胆固醇。 |
| 年龄（性别） | 雄性；11-13天 |
| 组织来源 | 睾丸间质 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| 受体表达情况 | Luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
| 基因表达情况 | prostaglandin F2a |
| 保藏机构 | ATCC; CRL-1714 ECACC; 91060526 |
STR鉴定
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STR位点信息
18-3 18 6-7 15 5-5 12 X-1 24 1-2 15,17 7-1 25.2,26.2 8-1 13,16,17 1-1 16 19-2 9 15-3 20.3,22.3 12-1 16 6-4 16.3,18 4-2 19.3 3-2 13 2-1 9,16 13-1 16.2
11-2 15,18 17-2 15,16 -
STR鉴定图
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参考文献
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Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders(2021/10/20)
作者：Tianxin Zhao, Xiangliang Tang, Dian Li
期刊：Journal of Hazardous Materials
DOI：10.1016/j.jhazmat.2021.127547
影响因子 ：14.224
引用产品：CL-0234
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Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells(2020/08/14)
作者：Jun Jing, Ning Ding, Dandan Wang
期刊：Cell Death & Disease
DOI：10.1038/s41419-020-02751-z
影响因子 ：9.000
引用产品：CL-0234
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Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking(2023/04/10)
作者：Jiachen Ge, Qi Qian, Yanhua Gao
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.154813
影响因子 ：7.900
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis(2023/07/20)
作者：Fangpeng Hou, Junyun Huang, Furong Qing
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2023.115262
影响因子 ：6.800
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Triphenyl phosphate induced apoptosis of mice testicular Leydig cells and TM3 cells through ROS-mediated mitochondrial fusion inhibition(2023/04/05)
作者：Minxin Wang, Jinyu Xu, Zhengbo Zhao
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2023.114876
影响因子 ：6.800
引用产品：CL-0234
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Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway(2020/2/13)
作者：Zhiqiang Li, Jing Zhao, Hongyu Liu
期刊：Life Sciences
影响因子 ：6.100
引用产品：CL-0234
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Phytochemical analysis and in vitro and in vivo antioxidant properties of Plagiorhegma dubia Maxim as a medicinal crop for diabetes treatment(2023/03/09)
作者：Hui Sun, Meihua Chen, Xu He
期刊：Arabian Journal of Chemistry
DOI：10.1016/j.arabjc.2023.104788
影响因子 ：6.000
引用产品：CL-0234
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1,25-Dihydroxyvitamin D deficiency accelerates male reproductive senescence in aging mice and 1,25(OH)2D3 alleviates oxidative stress via NF-κB/SOD(2021/4/7)
作者：Jialing He, Hui Wang, Jiaxin Shi
期刊：American Journal Of Physiology-Endocrinology And Metabolism
DOI：10.1152/ajpendo.00531.2020
影响因子 ：5.100
引用产品：CL-0234
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Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism(2019/8/25)
作者：Gaoqing Xu, Jing Zhao, Hongyu Liu
期刊：Molecules.
DOI：10.3390/molecules24173084.
影响因子 ：4.600
引用产品：CL-0234
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BMAL1 knockdown promoted apoptosis and reduced testosterone secretion in TM3 Leydig cell line(2020/4/16)
作者：He Ding, Jing Zhao, Hongyu Liu
期刊：Gene
DOI：10.1016/j.gene.2020.144672
影响因子 ：3.500
引用产品：CL-0234
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Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway(2022/01/08)
作者：Zhiqiang Li, Hongtao Wang, Kaiyan Zhang
期刊：Molecular Biology Reports
DOI：10.1007/s11033-021-07107-0
影响因子 ：2.800
引用产品：CL-0234
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Knockdown of ZnT4 Induced Apoptosis, Inhibited Proliferation and testosterone synthesis of TM3 cells(2023/09/21)
作者：Huanhuan Li, Yuejia Li, Junsheng Liu
期刊：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI：10.1007/s11626-023-00804-z
影响因子 ：2.100
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MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells(2023/01/30)
作者：Chengshuang Pan, Qianjin Fei, Jianyuan Jin
期刊：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI：10.1007/s11626-022-00745-z
影响因子 ：2.100
引用产品：CL-0234
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A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway(2021/11/30)
作者：Shiting Yu,Bing Han,Xin Xing
期刊：Oxidative Medicine and Cellular Longevity
影响因子 ：0.000
