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产品概述
| 名称 | C2C12 (小鼠成肌细胞) (种属鉴定正确) |
| 别称 | C2c12; C2-C12; C12 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成肌细胞样 |
| 生长培养基 | DMEM＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | C2C12细胞是由D·Yaffe和O·Saxel建立的小鼠成肌细胞株的亚克隆；C2C12细胞分化很快，形成可伸缩的肌管并生成特征性的肌蛋白。用骨形成蛋白2(BMP-2)处理C2C12细胞，会导致C2C12细胞的分化途径从成肌细胞转换为成骨细胞。检测表明，C2C12细胞肢骨发育畸形病毒(鼠痘)阴性。 |
| 年龄（性别） | 不详 |
| 组织来源 | 骨骼肌；品系：C3H |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~20小时 |
| 保藏机构 | ATCC; CRL-1772 BCRC; 60083 BCRJ; 0058 DSMZ; ACC-565 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15 X-1 25,26 1-2 16 7-1 26 8-1 17 1-1 10 19-2 12 15-3 25.3 12-1 16 6-4 18 4-2 19.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 15 -
STR鉴定图
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参考文献
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Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo(2022/02/10)
作者：Yi Zongyi, Qu LiangTang, Huixian Liu
期刊：Nature Biotechnology
DOI：10.1038/s41587-021-01180-3
影响因子 ：68.164
引用产品：CL-0044
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Polystyrene microplastics-induced ROS overproduction disrupts the skeletal muscle regeneration by converting myoblasts into adipocytes(2021/04/27)
作者：Shengchen Wang, Jing Liu, Yujie Yao
期刊：Journal Of Hazardous Materials
DOI：10.1016/j.jhazmat.2021.125962
影响因子 ：13.600
引用产品：CL-0044
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MOFs-Based Nanoagents Enable Sequential Damage to Cancer-Associated Fibroblast and Tumor Cells for Phototriggered Tumor Microenvironment Regulation(2023/08/31)
作者：Jiaqi Meng, Weier Bao, Ming Liu
期刊：Small
影响因子 ：13.300
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Nanoparticle-Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs(2022/03/04)
作者：Jie Tao, Shunyao Zhu, Nazi Zhou
期刊：Advanced Healthcare Materials
影响因子 ：11.092
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Cordycepin inhibits myogenesis via activating the ERK1/2 MAPK signalling pathway in C2C12 cells(2023/07/14)
作者：Chunfang Cheng, Shasha Zhang, Yanchun Gong
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.115163
影响因子 ：7.500
引用产品：CL-0044
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TNF-α contributes to sarcopenia through caspase-8/caspase-3/GSDME-mediated pyroptosis(2023/02/24)
作者：Jingying Wu, Siming Lin, Weixiao Chen
期刊：Cell Death Discovery
DOI：10.1038/s41420-023-01365-6
影响因子 ：7.000
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Enrichment of miR-17-5p enhances the protective effects of EPC-EXs on vascular and skeletal muscle injury in a diabetic hind limb ischemia model(2023/04/01)
作者：Qunwen Pan, Xiaobing Xu, Wen He
期刊：Biological Research
DOI：10.1186/s40659-023-00418-5
影响因子 ：6.700
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Near-infrared triggered ropivacaine liposomal gel for adjustable and prolonged local anaesthesia(2021/11/23)
作者：Yufei Hou, Xiangxue Meng, Shixin Zhang
期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2021.121315
影响因子 ：6.510
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Effect of manganese ion doping on magnetic properties and magnetic induction heating of Zn-Al ferrite nanoparticles(2023/07/31)
作者：Yifan Zheng, Renpeng Yang, Xiaogang Yu
期刊：JOURNAL OF ALLOYS AND COMPOUNDS
DOI：10.1016/j.jallcom.2023.171597
影响因子 ：6.200
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Targeting NAT10 protects against sepsis-induced skeletal muscle atrophy by inhibiting ROS/NLRP3(2023/07/17)
作者：Chuntao Wang, Yukun Liu, Yongsheng Zhang
期刊：LIFE SCIENCES
影响因子 ：6.100
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Reduced energy metabolism contributing to aging of skeletal muscle by serum metabolomics and gut microbiota analysis(2023/03/23)
作者：Pan He, Guanhua Du, Xuemei Qin
期刊：Life Sciences
影响因子 ：6.100
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miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1(2022/12/30)
作者：Lixue Wang, Yuhuai Xie, Wei Chen
期刊：Cells
影响因子 ：6.000
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Vitamin A Promotes the Repair of Mice Skeletal Muscle Injury through RARα(2023/08/22)
作者：Wenjia Zhang, Qingyun Peng, Xiaoyu Zhang
期刊：Nutrients
影响因子 ：5.900
引用产品：CL-0044
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GSDMD Knockout Alleviates Sepsis-Associated Skeletal Muscle Atrophy by Inhibiting IL18/AMPK Signaling(2023/06/22)
作者：Yongsheng Zhang, Yukun Liu, Chuntao Wang
期刊：iScience
影响因子 ：5.800
引用产品：CL-0044
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MiR‐499 regulates myoblast proliferation and differentiation by targeting transforming growth factor β receptor 1(2018/09/19)
作者：Jiyao Wu, Binglin Yue, Xianyong Lan
期刊：Journal of Cellular Physiology
影响因子 ：5.600
引用产品：CL-0044
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Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues(2021/09/20)
作者：Fuying Qin, Jiaojiao Zhang, Daiwei Wang
期刊：Journal of Functional Foods
影响因子 ：5.600
引用产品：CL-0044
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Aronia melanocarpa anthocyanin extracts are an effective regulator of suppressor of cytokine signaling 3-dependent insulin resistance in HepG2 and C2C12 cells(2020/10/28)
作者：Jing Chen, Jinyan Zhu, Xianjun Meng
期刊：Journal of Functional Foods
影响因子 ：5.600
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Meroterpenoids From Ganoderma lucidum Mushrooms and Their Biological Roles in Insulin Resistance and Triple-Negative Breast Cancer(2021/11/3)
作者：Jiaojiao Zhang, Daiwei Wang, Dan Cai
期刊：Frontiers in Chemistry
影响因子 ：5.500
引用产品：CL-0044
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Aerobic exercise and resistance exercise alleviate skeletal muscle atrophy through IGF-1/IGF-1R-PI3K/Akt pathway in mice with myocardial infarction(2021/12/01)
作者：Lili Feng, Bowen Li, Yue Xi
期刊：American Journal Of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00344.2021
影响因子 ：5.500
引用产品：CL-0044
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BMSC-Derived Exosomes Inhibit Dexamethasone-Induced Muscle Atrophy via the miR-486-5p/FoxO1 Axis(2021/10/01)
作者：Ziyi Li, Chang Liu, Shilun Li, Ting Li
期刊：Frontiers in Endocrinology
影响因子 ：5.200
引用产品：CL-0044
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MicroRNA transcriptome of skeletal muscle during yak development reveals miR-652 regulates myoblasts differentiation and survival by targeting ISL1(2022/08/27)
作者：Xuelan Zhou, Xian Guo, Chunnian Liang
期刊：Journal of Integrative Agriculture
影响因子 ：4.800
引用产品：CL-0044
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Proteoglycan Extracted from Ganoderma lucidum Ameliorated Diabetes-Induced Muscle Atrophy via the AMPK/SIRT1 Pathway In Vivo and In Vitro(2023/08/08)
作者：Jiaqi Li, Ying Zhang, Fanzhen Yu
期刊：ACS Omega
影响因子 ：4.100
引用产品：CL-0044
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Meroterpenoids containing benzopyran or benzofuran motif from Ganoderma cochlear(2022/04/09)
作者：Fuying Qin, Daiwei Wang, Te Xu
期刊：PHYTOCHEMISTRY
DOI：10.1016/j.phytochem.2022.113184
影响因子 ：3.800
引用产品：CL-0044
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Spiroganodermaines A−G from Ganoderma species and their activities against insulin resistance and renal fibrosis(2022/08/02)
作者：Jiaojiao Zhang, Daiwei Wang, Yunli Peng
期刊：PHYTOCHEMISTRY
DOI：10.1016/j.phytochem.2022.113324
影响因子 ：3.800
引用产品：CL-0044
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Development of Probiotic-Fermented Black Mulberry (Morus nigra L.) Juice and Its Antioxidant Activity in C2C12 Cells(2022/12/01)
作者：Mingshan Lv, Aihemaitijiang Aihaiti, Xiaolu Liu
期刊：Fermentation-Basel
DOI：10.3390/fermentation8120697
影响因子 ：3.700
引用产品：CL-0044
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Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells(2023/08/09)
作者：Xinyan Pan, Chunqiao Liu, Xing Wang
期刊：Molecular Medicine Reports
影响因子 ：3.400
引用产品：CL-0044
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LncRNA XLOC_015548 affects the proliferation and differentiation of myoblasts via the MAPK signaling pathway(2023/02/27)
作者：Yihao Wei, Tiantian Qi, Siyang Cao
期刊：EXPERIMENTAL BIOLOGY AND MEDICINE
影响因子 ：3.200
引用产品：CL-0044
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Low frequency pulsed electromagnetic field promotes C2C12 myoblasts proliferation via activation of MAPK/ERK pathway(2016/06/11)
作者：Haixia Xu, Jie Zhang, Yutian Lei
期刊：Biochemical and Biophysical Research Communications
DOI：10.1016/j.bbrc.2016.09.044
影响因子 ：3.100
引用产品：CL-0044
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Transcription factor EB enhances autophagy and ameliorates palmitate-induced insulin resistance at least partly via upregulating AMPK activity in skeletal muscle cells(2021/10/6)
作者：Ping Wang, Chun Guang Li, Xian Zhou
期刊：Clinical And Experimental Pharmacology And Physiology
影响因子 ：2.900
引用产品：CL-0044
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Structural characterization of minor optically pure and impure meroterpenoid-type compounds in Ganoderma lucidum and structure revision of spirolingzhine D(2022/09/17)
作者：Jiaojiao Zhang, Daiwei Wang, Yunli Peng
期刊：TETRAHEDRON
影响因子 ：2.100
引用产品：CL-0044
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脓毒症通过抑制AKT/GSK3β磷酸化导致骨骼肌细胞膜nAChRs聚集障碍(2019/11/30)
作者：Tianmei Li, Li Liu, Xiaobin Wang
期刊：南方医科大学学报
DOI：10.12122/j.issn.1673-4254.2019.11.11
影响因子 ：1.397
引用产品：CL-0044
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Interactions between Cytosolic Phospholipase A2 Activation and Mitochondrial Reactive Oxygen Species Production in the Development of Ventilator-Induced Diaphragm Dysfunction(2019/04/18)
作者：Xianlong Zhou, Xiaojun Wei, Shaoping Li
期刊：Oxidative Medicine and Cellular Longevity
影响因子 ：0.000
引用产品：CL-0044
