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| 名称 | MC3T3-E1 Subclone 14 (小鼠颅顶前骨细胞亚克隆14) (STR鉴定正确) |
| 别称 | MC3T3-E1 SUBCLONE 14 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆，从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化，不形成ECM，可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质，表达可比较的基本水平的mRNA编码Osf2/Cbfa1，一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后，高分化性的亚克隆形成与骨类似的形成小骨的编织骨，低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型，尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
| 年龄（性别） | 新生 |
| 组织来源 | 颅顶骨 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
| 基因表达情况 | collagen |
| 保藏机构 | ATCC; CRL-2594 |
STR鉴定
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STR位点信息
18-3 15 6-7 17 5-5 17 X-1 28 1-2 19 7-1 26.2 8-1 16 1-1 16,17 19-2 13 15-3 22.3 12-1 17 6-4 18 4-2 20.3 3-2 14 2-1 16 13-1 16.1
11-2 16 17-2 16 -
STR鉴定图
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参考文献
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期刊：Materials Today
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影响因子 ：24.200
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期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2021.10.017
影响因子 ：18.900
引用产品：CL-0378
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A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis(2021/09/17)
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期刊：Bioactive Materials
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影响因子 ：18.900
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An injectable self-adaptive polymer as a drug carrier for the treatment of nontraumatic early-stage osteonecrosis of the femoral head(2022/03/12)
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期刊：Bone Research
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影响因子 ：12.700
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期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.121666
影响因子 ：11.200
引用产品：CL-0378
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作者：Minggang Yang, Hui Liu, Caijie Qiu
期刊：Carbon
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影响因子 ：10.900
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期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.126145
影响因子 ：8.200
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作者：Renlei Yang, Jiao Zhang, Jie Li
期刊：Free Radical Biology And Medicine
DOI：10.1016/j.freeradbiomed.2021.12.010
影响因子 ：8.101
引用产品：CL-0378
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作者：Yang Qiu, Jianyue Ying, Fujie Yan
期刊：FOOD RESEARCH INTERNATIONAL
DOI：10.1016/j.foodres.2023.112850
影响因子 ：8.100
引用产品：CL-0378
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Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells(2021/05/10)
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期刊：International Journal of Nanomedicine
影响因子 ：8.000
引用产品：CL-0378
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影响因子 ：7.500
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Corylifol A protects against ovariectomized-induced bone loss and attenuates RANKL‐induced osteoclastogenesis via ROS reduction, ERK inhibition, and NFATc1 activation(2023/01/14)
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期刊：FREE RADICAL BIOLOGY AND MEDICINE
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影响因子 ：7.400
引用产品：CL-0378
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Amorphous Calcium Magnesium Phosphate Nanocomposites with Superior Osteogenic Activity for Bone Regeneration(2021/11/24)
作者：Jiang Yingying, Tan Shuo, Hu Jianping
期刊：Regenerative Biomaterials
影响因子 ：6.700
引用产品：CL-0378
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Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration(2021/7/19)
作者：Yuhan Zhang, Ting Wang, Juan Li
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：5.700
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IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA(2022/11/27)
作者：Zimo Zhou, Senxiang Chen, Tong Wu, Yifeng Chen, Yuxiao Cao, Ying Huang, Da Liu
期刊：JOURNAL OF CELLULAR PHYSIOLOGY
影响因子 ：5.600
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Dipeptidyl peptidase 3 is essential for maintaining osteoblastic differentiation under a high-glucose environment by inhibiting apoptosis, oxidative stress and inflammation through the modulation of the Keap1-Nrf2 pathway(2023/06/03)
作者：Xiaofeng Li, Xiangyou Yu, Shenglin He
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110404
影响因子 ：5.600
引用产品：CL-0378
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Er-xian decoction drug-containing serum promotes Mc3t3-e1 cell proliferation and osteogenic differentiation via regulating BK channel(2022/11/01)
作者：Ming-Shi Ren, Hui-Hui Xie, Yu Ding, Zi-Han Li, Bo Liu
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.400
引用产品：CL-0378
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Apocynin exerts cytoprotective effects on dexamethasone-induced osteoblasts by inhibiting oxidative stress through the Nrf2 signalling pathway(2023/09/25)
作者：Xinglong Zhang, Ran Pang, Kai Zhang
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 ：5.300
引用产品：CL-0378
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The effect of calcitonin gene-related peptide functionalized TiO2 nanotubes on osteoblast and osteoclast differentiation in vitro(2020/05/05)
作者：Min Lai, Xufeng Yan, Ke Shen
期刊：Colloids And Surfaces A-Physicochemical And Engineering Aspects
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影响因子 ：5.200
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Effects of a novel self-assembling peptide scaffold on bone regeneration and controlled release of two growth factors(2021/12/07)
作者：Hongchuan Tian, Ai Guo, Kai Li
期刊：Journal Of Biomedical Materials Research Part A
影响因子 ：4.900
引用产品：CL-0378
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Resveratrol Protects Osteoblasts Against Dexamethasone-Induced Cytotoxicity Through Activation of AMP-Activated Protein Kinase(2020/10/23)
作者：Liang Wang, Qiushi Li, Haibo Yan
期刊：Drug Design Development and Therapy
影响因子 ：4.800
引用产品：CL-0378
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Study of Injectable Hydrogel Based on ALN/Nha Promoting Osteogenesis And Inhibiting Osteoclasts in Osteoporotic Bone Defects Repair(2024/01/12)
作者：Lu Yang, Xingyu Chen, Long Chen
期刊：MACROMOLECULAR BIOSCIENCE
影响因子 ：4.600
引用产品：CL-0378
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A novel fluorescent probe for detecting hydrogen sulfide in osteoblasts during lipopolysaccharide-mediated inflammation under periodontitis(2021/10/11)
作者：Xiaoya Lu , Hanchuang Zhu , Yi Chen
期刊：Scientific Reports
DOI：10.1038/s41598-021-99761-4
影响因子 ：4.600
引用产品：CL-0378
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Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane(2022/05/03)
作者：Qiuhong Zhu, Hua Jiao, Xiaoliang Zhao
期刊：RSC Advances
影响因子 ：4.036
引用产品：CL-0378
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Microstructured titanium functionalized by naringin inserted multilayers for promoting osteogenesis and inhibiting osteoclastogenesis(2021/08/10)
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期刊：Journal Of Biomaterials Science-Polymer Edition
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影响因子 ：3.600
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D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation(2019/01/05)
作者：WeiKang Zhao, Bin He, Ao Zhou
期刊：Tissue Engineering and Regenerative Medicine
影响因子 ：3.600
引用产品：CL-0378
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Comprehensive reparative effects of bacteriostatic poly(L-lactide-co-glycolide)/poly(L-lactide-co-ε-caprolactone) electrospinning membrane on alveolar bone defects in progressive periodontitis(2022/10/05)
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影响因子 ：3.400
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Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds(2018/11/27)
作者：Yanbo Zhang, Ruiyan Li, Wenzheng Wu
期刊：Biomed Research International
影响因子 ：3.246
引用产品：CL-0378
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The initial application of conch-shell hydroxyapatite and HACC as functional filler in PMMA bone cement(2023/08/06)
作者：Luqi Tian, Ronge Xing, Haoyue Yang
期刊：International Journal of Polymeric Materials and Polymeric Biomaterials
DOI：10.1080/00914037.2023.2243367
影响因子 ：3.200
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Fabrication and Characterization of Suspension Plasma-Sprayed Fluoridated Hydroxyapatite Coatings for Biomedical Applications(2018/8/20)
作者：Yu Bai, Shengjian Zhou, Li Shi
期刊：J Therm Spray Tech
影响因子 ：3.100
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Fabrication of porous gehlenite coating on Al2O3–ZrO2–SiC composite ceramics and its in vitro biological activities:(2022/03/24)
作者：Xianzhi Chen, Jiaxin Li, Shuidan Gu
期刊：Journal Of Biomaterials Applications
影响因子 ：2.900
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Irisin enhances osteogenic differentiation of mouse MC3T3‑E1 cells via upregulating osteogenic genes(2021/5/31)
作者：Jian Yang, Kai Yu, Dongmei Liu
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
引用产品：CL-0378
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MiR-708 inhibits MC3T3-E1 cells against H2O2-induced apoptosis through targeting PTEN(2020/7/10)
作者：Wei Zhang, Shengyu Cui, Hong Yi
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-020-01780-w
影响因子 ：2.600
引用产品：CL-0378
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Composition and bioactivity of calcium phosphate coatings on anodic oxide nanotubes formed on pure Ti and Ti-6Al-4V alloy substrates(2020/01/23)
作者：Qianting Yao, Yingying Jiang, Shuo Tan
期刊：Materials Science and Engineering: C
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影响因子 ：1.900
引用产品：CL-0378
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Biomimetic Synthesis and Mechanism of Hydroxyapatite Crystals by Biotemplate of Small Intestinal Submucosa and Bacterial Cellulose(2023/06/25)
作者：Qiuhong Zhu, Hua Jiao, Kang Zhao
期刊：CRYSTAL RESEARCH AND TECHNOLOGY
影响因子 ：1.500
引用产品：CL-0378
