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产品概述
| 名称 | MCF 10A (人正常乳腺上皮细胞) (STR鉴定正确) |
| 别称 | MCF 10A; MCF.10A; MCF10A; MCF10a; MCF-10 Attached; Michigan Cancer Foundation-10A |
| 种属 | 人类 |
| 年龄（性别） | 36岁 |
| 组织来源 | 乳腺；乳房 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生物安全等级 | 1 |
| 生长培养基 | DMEM/F12＋5% HS(164215)＋20ng/ml EGF＋0.5μg/ml Hydrocortisone＋10μg/ml Insulin＋1% NEAA＋1% P/S |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 注意事项 | 该细胞较难消化，注意延长消化时间，消化到细胞收缩变圆，轻拍培养瓶侧边，细胞可以滑落方可终止消化；通常需要10~15分钟。 |
| 保藏机构 | ATCC; CRL-10317 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 21,26 D3S1358 14,18 D5S818 10,13 D7S820 10,11 D8S1179 14,16 D13S317 8,9 D16S539 11,12 D18S51 18,19 D19S433 13,15 D21S11 28,30 FGA 22,24 PentaD 10,12 PentaE 13,14 TH01 8,9.3 TPOX 9,11 vWA 15,17 D6S1043 12,18 D12S391 17,20 D2S441 11,14 -
STR鉴定图
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参考文献
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Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging(2019/8/12)
作者：Zhongyuan Chen, Yueting Peng, Xiaoxue Xie
期刊：Theranostics
影响因子 ：11.556
引用产品：CL-0525
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An endogenous stimulus detonated nanocluster-bomb for contrast-enhanced cancer imaging and combination therapy(2021/8/17)
作者：Huanhuan Sun, Wenjie Ma, Shuangdi Duan
期刊：Chemical Science
影响因子 ：9.825
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Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer(2021/4/19)
作者：Xinxing Wang, Bingjian Xue, Yujie Zhang
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-03684-x
影响因子 ：8.469
引用产品：CL-0040 CL-0041 CL-0149 CL-0150A CL-0150B CL-0152A CL-0152B CL-0290A CL-0290B CL-0525
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MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis(2021/4/6)
作者：Fengxia Chen, Qingqing Wang, Xiaoyan Yu
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-03661-4
影响因子 ：8.469
引用产品：CL-0005 CL-0041 CL-0114 CL-0150A CL-0150B CL-0380 CL-0525 PM150312B
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Simultaneous Fluorescence Visualization of Epithelial-Mesenchymal Transition and Apoptosis Processes in Tumor Cells for Evaluating the Impact of Epithelial-Mesenchymal Transition on Drug Efficacy(2018/9/18)
作者：Mingming Luan, Jinjie Chang, Wei Pan
期刊：Anal Chem
DOI：10.1021/acs.analchem.8b02494
影响因子 ：6.986
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AFM Force Relaxation Curve Reveals That the Decrease of Membrane Tension Is the Essential Reason for the Softening of Cancer Cells(2021/5/12)
作者：Keli Ren, Jingwei Gao, Dong Han
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：6.684
引用产品：CL-0525
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Multifunctional Hf/Mn-TCPP Metal-Organic Framework Nanoparticles for Triple-Modality Imaging-Guided PTT/RT Synergistic Cancer Therapy(2020/10/18)
作者：Jianfeng Bao, Xiangyang Zu, Xiao Wang
期刊：International Journal of Nanomedicine
影响因子 ：6.4
引用产品：CL-0525
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Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway(2019/10/07)
作者：Tianqi Li, Yudi Xiong, Qingqing Wang
期刊：Artificial Cells Nanomedicine and Biotechnology
DOI：10.1080/21691401.2019.1670670
影响因子 ：5.678
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Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway(2019.10.07)
作者：Tianqi Li , Yudi Xiong, Qingqing Wang
期刊：Artificial Cells Nanomedicine and Biotechnology
DOI：10.1080/21691401.2019.1670670
影响因子 ：5.678
引用产品：CL-0525
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Enzymatic rhamnosylation of anticancer drugs by an α-L-rhamnosidase from Alternaria sp. L1 for cancer-targeting and enzyme-activated prodrug therapy(08 August 2019)
作者：Li Xu, Xiaohong Liu, Yinping Li, Zhenhao Yin
期刊：Applied Microbiology and Biotechnology
DOI：10.1007/s00253-019-10011-0
影响因子 ：4.813
引用产品：CL-0525
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Enzymatic rhamnosylation of anticancer drugs by an α-l-rhamnosidase from Alternaria sp. L1 for cancer-targeting and enzyme-activated prodrug therapy(2019/08/08)
作者：Li Xu , Xiaohong Liu , Yinping Li
期刊：Applied Microbiology And Biotechnology
DOI：10.1007/s00253-019-10011-0
影响因子 ：4.813
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Ferroptosis regulators, especially SQLE, play an important role in prognosis, progression and immune environment of breast cancer(2021/10/29)
作者： Wenqing Tang, Fangshi Xu, Meng Zhao
期刊：Bmc Cancer
DOI：10.1186/s12885-021-08892-4
影响因子 ：4.43
