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![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-1.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-2.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-3.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-4.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-5.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0137-6.jpg)
![NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | NCTC clone 929 [L cell, L-929] (小鼠成纤维细胞) (STR鉴定正确) |
| 别称 | NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 生长培养基 | MEM（含NEAA）＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | NCTC clone 929 [L cell, L-929, derivative of Strain L-929]细胞是1948年3月建立的细胞系L的克隆株；细胞系L是最早建立的连续培养细胞系之一，而NCTC clone 929 [L cell, L-929, derivative of Strain L-929]是最早的克隆株。从一只100日龄的雄性C3H/An小鼠的皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L，第95代的细胞系L使用毛细管法分离单细胞建立了NCTC clone 929 [L cell, L-929, derivative of Strain L-929]。 |
| 年龄（性别） | 雄性，100日龄 |
| 组织来源 | 未知 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~28-36小时 |
| 致瘤性 | Yes, in immunosuppressed mice. |
| 保藏机构 | ATCC; CCL-1 ATCC; CRL-6364BCRC; 60091 BCRJ; 0188 DSMZ; ACC-2 ECACC; 85011425 ECACC; 85103115 ECACC; 88102702 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 14 X-1 26,27 1-2 17 7-1 25,26,27 8-1 16 1-1 10 19-2 12 15-3 24.3,25.3,26.3 12-1 16 6-4 17,18 4-2 20.3 3-2 13,14 2-1 9 13-1 17.1
11-2 15,16 17-2 15 -
STR鉴定图
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参考文献
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期刊：INORGANIC CHEMISTRY COMMUNICATIONS
DOI：10.1016/j.inoche.2023.110543
影响因子 ：3.800
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Microwave-Assisted Synthesis of Luminescent Carbonaceous Nanoparticles as Silkworm Feed for Fabricating Fluorescent Silkworm Silk(2022/12/24)
作者：Wenkai Chen, Yangsheng Zhong, Gangrong Fu
期刊：Coatings
影响因子 ：3.400
引用产品：CL-0137
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Mussel‐mimetic chitosan based injectable hydrogel with fast-crosslinking and water-resistance as tissue adhesive(2023/04/24)
作者：Yurui Wang, Jing Zhao, Xiaoran Wang
期刊：INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
DOI：10.1016/j.ijadhadh.2023.103382
影响因子 ：3.400
引用产品：CL-0137
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A New 7-azaindole Structure Analog: Molecular Docking, Synthesis and Preliminary Biological Activity in vitro for Anticancer(2022/09/09)
作者：Yifeng Zhan, Hang Tong, Shibo He
期刊：CHEMISTRY & BIODIVERSITY
影响因子 ：2.900
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Nanofiber Induced Silk Fibroin Nanofiber/Silk Fibroin (SFNF/SF) Fibrous Scaffolds for 3D Cell Culture(2023/02/17)
作者：Shiyang Chen, Tongda Lei, Yunrui Zhang
期刊：FIBERS AND POLYMERS
DOI：10.1007/s12221-023-00113-y
影响因子 ：2.500
引用产品：CL-0137
