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产品概述
| 名称 | NIH/3T3 (小鼠胚胎细胞) (STR鉴定正确) |
| 别称 | NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3 |
| 背景描述 | NIH/3T3细胞是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。NIH/3T3细胞对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用；NIH/3T3细胞鼠痘病毒阴性。 |
| 种属 | 小鼠 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胚胎 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | DMEM＋10% CS＋1% P/S |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~20小时 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 保藏机构 | ATCC; CRL-1658 ATCC; CRL-6442DSMZ; ACC-59 ECACC; 93061524 |
STR鉴定
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STR位点信息
18-3 17,19 6-7 12 5-5 14,15 X-1 25 1-2 13,17 7-1 29 8-1 15 1-1 10 19-2 11,12 15-3 20.3 12-1 20 6-4 16 4-2 19.3,20.3 3-2 14,15 2-1 9 13-1 16.2 11-2 15,17 17-2 13,14 -
STR鉴定图
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参考文献
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Triple Enzyme-Regulated Molecular Hydrogels for Carrier-Free Delivery of Lonidamine(2021/07/29)
作者：Can Wu, Chunlu Wang, Yaoyao Zheng
期刊：Advanced Functional Materials
影响因子 ：19.9240
引用产品：CL-0171
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Triple Enzyme-Regulated Molecular Hydrogels for Carrier-Free Delivery of Lonidamine(2021/07/29)
作者：Can Wu, Chunlu Wang, Yaoyao Zheng
期刊：Advanced Functional Materials
影响因子 ：19.9240
引用产品：CL-0171
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Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells(2022/08/12)
作者：Qi Wang, Feng Xiao, Haomiao Su
期刊：NUCLEIC ACIDS RESEARCH
影响因子 ：19.1600
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Genetically engineered bi-functional silk material with improved cell proliferation and anti-inflammatory activity for medical application(2018/12/23)
作者：Yuancheng Wang, Feng Wang, Sheng Xu
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.12.036
影响因子 ：10.633
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Eco-friendly and degradable red phosphorus nanoparticles for rapid microbial sterilization under visible light(2020/07/26)
作者：Qian Wu, Xiangmei Liu, Bo Li
期刊：Journal of Materials Science & Technology
DOI：10.1016/j.jmst.2020.04.084
影响因子 ：10.319
引用产品：CL-0171
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MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression(2022/01/12)
作者：Jinti Lin, Ankai Xu, Jiakang Jin
期刊：OncoImmunology
DOI：10.1080/2162402X.2021.2024941
影响因子 ：7.7230
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A confined crosslinking strategy towards an intelligent organosilica–micellar hybrid drug delivery system(2022/1/18)
作者：Xing Qin, Limei Qin, Jianping He
期刊：Biomaterials Science
影响因子 ：7.5900
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Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing(2022/08/17)
作者：Hongli Zhang, Xu Zhang, Qi Cao
期刊：Biomaterials Science
影响因子 ：7.5900
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The miR-21-5p enriched in the apoptotic bodies of M2 macrophage-derived extracellular vesicles alleviates osteoarthritis by inducing a change in the macrophage phenotype(2022/10/05)
作者：Leilei Qin, Jianye Yang, Xudong Su
期刊：Genes & Diseases
DOI：10.1016/j.gendis.2022.09.010
影响因子 ：7.2430
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Danshensu alleviates bleomycin-induced pulmonary fibrosis by inhibiting lung fibroblast-to-myofibroblast transition via the MEK/ERK signaling pathway(2021/6/30)
作者：Huaman Liu, Xinyue Zhang, Yumeng Shao
期刊：Bioengineered
DOI：10.1080/21655979.2021.1944020
影响因子 ：6.8320
引用产品：CL-0171
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Mussel-inspired biocompatible polydopamine/carboxymethyl cellulose/polyacrylic acid adhesive hydrogels with UV-shielding capacity(2021/01/02)
作者：Zuwu Tang, Yanan Miao, Jing Zhao
期刊：Cellulose
DOI：10.1007/s10570-020-03596-7
影响因子 ：6.1230
引用产品：CL-0171
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Triggering Drug Release and Thermal-Disrupting Interface Induced Mitigation of Composite Photothermal Hydrogel Treating Infectious Wounds( 2021/12/13)
作者：Long Hua, Hu Qian, Ting Lei
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：6.0640
引用产品：CL-0171
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Allyl phenyl selenides as H2O2 acceptors to develop ROS-responsive theranostic prodrugs(2022/09/17)
作者：Xueyan Yang, Bo Yuan, Hai Xiong
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2022.106154
影响因子 ：5.3070
引用产品：CL-0171
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microRNA‐542‐5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen‐activated protein kinase signaling pathway through the negative regulation of P21‐activated kinase 1(2018/9/14)
作者：Xingmao Wu, Kaiqiang Ji, Haiyuan Wang
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.4800
引用产品：CL-0171
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Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers(2022/10/20)
作者：Hui Liu, Xiao Liu, Yuanqiao Cao
期刊：CHINESE JOURNAL OF POLYMER SCIENCE
影响因子 ：3.8150
引用产品：CL-0171
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Adjusting the accuracy of PEGDA-GelMA vascular network by dark pigments via digital light processing printing:(2021/11/05)
作者：Lin Sheng, Mo Li, Shuxian Zheng
期刊：Journal Of Biomaterials Applications
影响因子 ：2.7120
引用产品：CL-0171
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Study on properties and biocompatibility of poly (butylene succinate) and sodium alginate biodegradable composites for biomedical applications(2022/08/26)
作者：Shanshan Wang, Quansheng Xing
期刊：Materials Research Express
影响因子 ：2.0250
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Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation(2017/08/07)
作者：Fengming Zhu, Octavia L S Chong Lee Shin, Guangchang Pei
期刊：Oncotarget
影响因子 ：0.0000
引用产品：CL-0171
