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![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-1.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-2.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-3.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-4.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-5.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-6.jpg)
![SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | SW480 [SW-480] (人结肠癌细胞) (DMEM) (STR鉴定正确) |
| 别称 | SW-480; SW 480; SW480E |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | DMEM＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | SW480 [SW-480]细胞源自原位直肠腺癌，和SW620细胞源自同一病人一年后的淋巴结转移。CSAp和直肠抗体3阴性；角蛋白免疫过氧化物酶染色阳性。p53基因第273位密码子的G→A突变引起Arg→His替代，309位密码子的C→T突变导致Pro→Ser替代。细胞p53蛋白表达水平提高，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性，癌基因N-myc的表达未做检测。SW480 [SW-480]细胞不表达细胞溶解酶，一种与肿瘤入侵相关的金属蛋白酶。有报道称，SW480 [SW-480]细胞表达GM-CSF。SW480 [SW-480]细胞ras原癌基因的12位密码子有一个突变，可以用作PCR法检测该突变的阳性对照。1978年11月，A·Leibovitz将其提交给ATCC时已传代至第91代。 |
| 年龄（性别） | 男性，50岁 |
| 组织来源 | 直肠；结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~30-50小时 |
| 致瘤性 | Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
| 受体表达情况 | epidermal growth factor (EGF) |
| 基因表达情况 | carcinoembryonic antigen (CEA) 0.7 ng/10^6 cells/10 days; keratin; transforming growth factor beta, myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -, HLA A2, B8, B17; Blood Type A; Rh+, The cells are positive for keratin by immunoperoxidase staining., The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. |
| 保藏机构 | ATCC; CCL-228 DSMZ; ACC-313 ECACC; 87092801 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 15 D5S818 13 D7S820 8 D8S1179 13 D13S317 12 D16S539 13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鉴定图
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参考文献
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Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer(2022/08/28)
作者：Meng Tang, Hui Xu, Hongyan Huang
期刊：Advanced Science
影响因子 ：15.100
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Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells(2018/04/24)
作者：Jingjing Mou, Jin Peng, Yingying Shi
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2018.04.099
影响因子 ：7.500
-
Design, synthesis and evaluation of N3-substituted quinazolinone derivatives as potential Blooms Syndrome protein (BLM) helicase inhibitor for sensitization treatment of colorectal cancer(2022/11/21)
作者：Jia-Li Tu, Bi-Han Wu, Heng-Bo Wu, Jia-En Wang, Zi-Lin Zhang, Kun-Yu Gao, Lu-Xuan Zhang, Qin-Rui Chen, Ying-Chen Zhou, Jia-Heng Tan, Zhi-Shu Huang, Shuo-Bin Chen
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2022.114944
影响因子 ：6.700
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Design, synthesis, and biological evaluation of 1,6-naphthyridine-2-one derivatives as novel FGFR4 inhibitors for the treatment of colorectal cancer(2023/08/04)
作者：Bo Fang, Yinshuang Lai, Hao Yan
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2023.115703
影响因子 ：6.700
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Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer(2021/11/06)
作者：Zhipeng Jiang, Hao Hu, Wenli Hu
期刊：Cancer Cell International
DOI：10.1186/s12935-021-02290-3
影响因子 ：5.800
引用产品：164210 164210-50 CL-0096 CL-0144 CL-0223B CL-0225B PB180120 PM150110 PM150210
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Galloflavin Relieves the Malignant Behavior of Colorectal Cancer Cells in the Inflammatory Tumor Microenvironment.( 2021/12/10)
作者：Li Guo, Yi Yang, Yongjia Sheng
期刊：Frontiers in Pharmacology
影响因子 ：5.600
引用产品：CL-0223B
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Palmitic acid-induced ferroptosis via CD36 activates ER stress to break calcium-iron balance in colon cancer cells(2023/03/12)
作者：Hao Kuang, Xuehua Sun, Ying Liu
期刊：FEBS Journal
影响因子 ：5.400
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Characterization of SHCBP1 to prognosis and immunological landscape in pan-cancer: novel insights to biomarker and therapeutic targets(2023/03/14)
作者：Fei Jiang, Yanlong Shi, Yue Wang
期刊：Aging-US
影响因子 ：5.200
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KIF18A improves migration and invasion of colorectal cancer (CRC) cells through inhibiting PTEN signaling(2023/09/13)
作者：Yuan Liu, Ming Sun, Bin Zhang
期刊：Aging-US
影响因子 ：5.200
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Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer(2023/06/05)
作者：Qian Kang, Xin Guo, Tianfu Li
期刊：Frontiers in Microbiology
DOI：10.3389/fmicb.2023.1192900
影响因子 ：5.200
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Long non-coding RNA HOXA-AS3 facilitates the malignancy in colorectal cancer by miR-4319/SPNS2 axis(2021/10/21)
作者：Yang Jiang, Xiaoyu Yu, Huixin Sun
期刊：Journal Of Physiology And Biochemistry
DOI：10.1007/s13105-021-00832-x
影响因子 ：5.080
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Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway(2023/09/15)
作者：Xintian Lan, Meng Lu, Xiaoxue Fang
期刊：MOLECULES
影响因子 ：4.600
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Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer(2020/06/28)
作者：Dingsheng Liu, Hong Zhang, Mingming Cui
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1783058
影响因子 ：4.300
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影响因子 ：4.000
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Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway(2023/06/22)
作者：Weiwei Zheng, Yingchang Guo, Guangtan Zhang
期刊：PLoS One
DOI：10.1371/journal.pone.0287133
影响因子 ：3.700
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者：Linping Lei, Xuqin Xie, Long He
期刊：Investigational New Drugs
DOI：10.1007/s10637-020-01014-0
影响因子 ：3.400
引用产品：CL-0096 CL-0118 CL-0144 CL-0196 CL-0223A CL-0223B CL-0225A CL-0225B
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Anti-cancer effects of Porphyra haitanensis polysaccharides on human colon cancer cells via cell cycle arrest and apoptosis without causing adverse effects in vitro(2020/8/11)
作者：WanZi Yao, Suresh Veeraperumal, HuaMai Qiu
期刊：3 BIOTECH
DOI：10.1007/s13205-020-02379-y
影响因子 ：2.800
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis(2021/8/10)
作者：HaiLin Xiong, XiaoHua Zhong, XiaoHong Guo
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
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Circ_0000370 Plays an Oncogenic Role in Colorectal Cancer by Regulating the miR-502-5p/SIRT1 Axis(2023/08/10)
作者：Zhu Li, Gangling Tong, Xiaodan Peng
期刊：BIOCHEMICAL GENETICS
DOI：10.1007/s10528-023-10468-9
影响因子 ：2.400
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NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer(2023/02/27)
作者：Hong Yang, Leilei Pu, Ruobing Li
期刊：Journal of Gastrointestinal Oncology
影响因子 ：2.100
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The combination of inositol hexaphosphate and inositol inhibits metastasis of colorectal cancer cells by upregulating claudin 7(2023/06/08)
作者：Yisa Han, Tongtong Lan, Xuezhen Ma
期刊：BIOLOGICAL & PHARMACEUTICAL BULLETIN
影响因子 ：2.000
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Cryptotanshinone, a Potential SIRT3 Inhibitor, Suppresses Colorectal Cancer Proliferation in vitro and in vivo(2023/08/18)
作者：Dimeng Song, Xiaoxue Fang, Zhiyuan Sun
期刊：Natural Product Communications
影响因子 ：1.800
