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产品概述
名称 | BEAS-2B(人正常肺上皮细胞)(STR鉴定正确) |
别称 | Beas-2B; BEAS 2B; BEAS2B; Beas2B; Bronchial Epithelium transformed with Ad12-SV40 2B |
背景描述 | BEAS-2B细胞是从一位非癌个体的正常人支气管上皮病理切片分离出的上皮细胞。这些细胞用腺病毒12-SV40病毒杂交病毒感染并克隆,BEAS-2B细胞保留了对血清反应进行鳞关分化的能力,并有用于筛选诱导或影响分化及致癌的化学或生物制剂。BEAS-2B细胞角蛋白及SV40抗原染色阳性。 |
种属 | 人 |
年龄(性别) | 男性 |
组织来源 | 肺;支气管;正常;上皮;病毒转化 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
细胞类型 | 转化细胞系 |
生物安全等级 | 2 |
生长培养基 | DMEM+10% FBS+1% P/S |
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO₂,5% 温度:37℃ |
致瘤性 | No, the cells were not tumorigenic in immunosuppressed mice |
保藏机构 | AddexBio; T0007001/26ATCC; CRL-9609BCRJ; 0395CCTCC; GDC0139ECACC; 95102433KCB; KCB 200922YJKerafast; ENH021-FPNCBI_Iran; C561 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 9,12 D2S1338 22,23 D3S1358 15,17 D5S818 12,13 D7S820 10,13 D8S1179 13,15 D13S317 13,13 D16S539 12,12 D18S51 18,19 D19S433 13.2,15.2 D21S11 28,30 FGA 20,24 PentaD 2.2,13 PentaE 5,8 TH01 7,9.3 TPOX 6,11 vWA 17,18 D6S1043 12,18 D12S391 17,18 D2S441 11,11.3 -
STR鉴定图
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参考文献
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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma(2021/070/7)
作者:Xiaoxia Dong, Yongyu Liu, Xinzhou Deng
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.081
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STIM1 mediates IAV-induced inflammation of lung epithelialcells by regulating NLRP3 and inflammasome activation viatargeting miR-223(2020/12/02)
作者:Cuicui Liu, Yi Miao, Ruilin Chen
期刊:Life Sciences
影响因子 :5.738
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Polydatin attenuates reactive oxygen species-induced airway remodeling by promoting Nrf2-mediated antioxidant signaling in asthma mouse model(2019/02/01)
作者:Haizhu Zeng, Yumeng Wang, Yuxia Gu
期刊:Life Sciences
影响因子 :5.738
引用产品:CL-0496
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者:Chao Yang, Jiang Shi, Jie Wang
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1941612
影响因子 :5.173
引用产品:164210 164210-50 CL-0165 CL-0496 CL-0519 PB180120 PM150110
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MicroRNA-30a Targets ATG5 and Attenuates Airway Fibrosis in Asthma by Suppressing Autophagy(2019/11/20)
作者:Binbin Li, Yunlong Chen, Fuzhen Pang
期刊:Inflammation
DOI:10.1007/s10753-019-01076-0
影响因子 :4.6570
引用产品:CL-0496
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The Protective Effect of Naringenin on Airway Remodeling after Mycoplasma Pneumoniae Infection by Inhibiting Autophagy-Mediated Lung Inflammation and Fibrosis(2018/04/04)
作者:Yan Lin, Dan Tan, Qianna Kan
期刊:Mediators Of Inflammation
影响因子 :4.529
引用产品:CL-0496
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Polydatin suppresses the development of lung inflammation and fibrosis by inhibiting activation of the NACHT domain‐, leucine‐rich repeat‐, and pyd‐containing protein 3 inflammasome and the nuclear factor‐κB pathway after Mycoplasma pneumoniae infect(2018/12/12)
作者:Jin Tang, Yungai Li, Jianqiang Wang
期刊:J Cell Biochem
影响因子 :4.480
引用产品:CL-0496
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Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis(2020/09/16)
作者:Yurong Jin, Zhenjun Su, Hui Sheng
期刊:Neoplasma
DOI:10.4149/neo_2020_200331N333
影响因子 :3.4090
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Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer(2021/10/29)
作者:Kui Xiao, Yang Wang, Lihua Zhou
期刊:PLoS One
DOI:10.1371/journal.pone.0259091
影响因子 :3.2400
引用产品:CL-0496
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MicroRNA-107 may regulate lung cancer cell proliferation and apoptosis by targeting TP53 regulated inhibitor of apoptosis 1(2020/1/7)
作者:Peng Cai, Jingjing Li,Guiming Chen
期刊:Oncology Letters
影响因子 :3.1110
引用产品:CL-0496
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CircRNA circ_0006892 regulates miR-24/PHLPP2 axis to mitigate cigarette smoke extract-induced bronchial epithelial cell injury(2021/5/18)
作者:Chenying Zhang, Shuangshuang Gu, Xiuwen Kang
期刊:BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
影响因子 :2.7240