产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-1.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-2.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-3.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-4.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-5.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/xbx/CL-0598-6.jpg)
![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (种属鉴定正确) |
| 别称 | bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞在低密度下形态不规则，细胞数量少，铺展面积大，高密度后形态会趋于一致，胞体密集；2.细胞增殖偏慢，初次传代建议1:2，细胞稳定后建议传代比例也不超过1:4；3.注意观察培养基颜色，颜色偏紫会导致细胞漂浮增加；4.消化时间会随着细胞生长时间和细胞密度而有所不同，需要结合消化时细胞状态来判断，消化到细胞可以滑落方可终止。 |
| 背景描述 | The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen |
| 年龄（性别） | 6周龄 |
| 组织来源 | 大脑皮层 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-1 |
| 抗原表达情况 | ICAM-1 +; VCAM-1 +; MAdCAM-1 + |
| 基因表达情况 | von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd |
| 保藏机构 | ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929 |
STR鉴定
-
STR位点信息
18-3 18 6-7 11,12 5-5 14 X-1 26 1-2 13 7-1 29 8-1 16 1-1 10 19-2 13 15-3 19.3,20.3 12-1 17 6-4 17 4-2 18.3 3-2 14 2-1 9 13-1 16.2
11-2 14 17-2 12 -
STR鉴定图
-
参考文献
-
A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis(2021/09/17)
作者：Yongzhi Cui, Yuanyuan Guo, Li Kong
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2021.09.015
影响因子 ：18.900
-
Targeted Delivery of RGD-CD146+CD271+ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury(2023/09/11)
作者：Yong Xie, Yi Sun, Yudong Liu
期刊：ACS Nano
影响因子 ：17.100
引用产品：CL-0598
-
A nitric-oxide driven chemotactic nanomotor for enhanced immunotherapy of glioblastoma(2023/02/20)
作者：Huan Chen, Ting Li, Zhiyong Liu
期刊：Nature Communications
DOI：10.1038/s41467-022-35709-0
影响因子 ：16.600
-
C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer’s disease(2023/09/15)
作者：Xiuhua Yin, Hong Zhou, Mengling Zhang
期刊：Nature Communications
DOI：10.1038/s41467-023-41489-y
影响因子 ：16.600
引用产品：CL-0598
-
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides(2022/08/06)
作者：Zhanchi Zhu, Yuanxin Zhai, Ying Hao
期刊：Journal of Extracellular Vesicles
影响因子 ：16.000
-
Gold nanocluster & indocyanine green based triple-effective therapy for MRSA infected central nervous system(2022/04/04)
作者：Yi Zhuo, Yi Zhang, Bing Wang
期刊：Applied Materials Today
DOI：10.1016/j.apmt.2022.101453
影响因子 ：8.300
-
Protection of a novel velvet antler polypeptide PNP1 against cerebral ischemia-reperfusion injury(2023/07/13)
作者：Hongyan Pei, Rui Du, Zhongmei He
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125815
影响因子 ：8.200
-
The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals(2017/02/17)
作者：Yahua Wang, Xue Ying, Haolun Xu
期刊：International Journal of Nanomedicine
影响因子 ：8.000
引用产品：CL-0598
-
Biosynthetic Gas Vesicles Combined with Focused Ultrasound for Blood–Brain Barrier Opening(2022/12/28)
作者：Jinghan Zhang, Fei Yan, Wei Zhang
期刊：International Journal of Nanomedicine
影响因子 ：8.000
引用产品：CL-0598
-
Active fraction of Polyrhachis vicina (Roger) alleviated cerebral ischemia/reperfusion injury by targeting SIRT3-mediated mitophagy and angiogenesis(2023/09/18)
作者：Jie Wei, Jiaxiu Xie, Junhui He
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.155104
影响因子 ：7.900
引用产品：CL-0598
-
Elabela-APJ axis mediates angiogenesis via YAP/TAZ pathway in cerebral ischemia/reperfusion injury(2023/02/21)
作者：Wenyu Li, Pengfei Xu, Lingqi Kong
期刊：Translational Research
DOI：10.1016/j.trsl.2023.02.002
影响因子 ：7.800
引用产品：CL-0598
-
Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression(2022/10/28)
作者：Bowen Zhao, Yueqi Ni, Hong Zhang
期刊：Cell Death Discovery
DOI：10.1038/s41420-022-01222-y
影响因子 ：7.000
-
Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma(2021/01/27)
作者：Lin Zhao, Hongyuan Chen, Lu Lu
期刊：Life Sciences
影响因子 ：6.100
引用产品：CL-0598
-
Mechanism of neocryptotanshinone in protecting against cerebral ischemic injury: By suppressing M1 polarization of microglial cells and promoting cerebral angiogenesis(2023/02/09)
作者：Liping Zhai, Hongyan Pei, Heping Shen
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.109815
影响因子 ：5.600
-
MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy(2023/11/30)
作者：Yongri Hong, Caichuan Wei, Miaoying Fu
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.111236
影响因子 ：5.600
引用产品：CL-0598
-
USP14 inhibition promotes recovery by protecting BBB integrity and attenuating neuroinflammation in MCAO mice(2023/06/02)
作者：Wenzhong Hou, Jianping Yao, Junjie Liu
期刊：CNS Neuroscience & Therapeutics
影响因子 ：5.500
引用产品：CL-0598
-
Inhibition of HDAC6 promotes microvascular endothelial cells to phagocytize myelin debris and reduces inflammatory response to accelerate the repair of spinal cord injury(2023/08/29)
作者：Chengjie Wu, Yalan Pan, Lining Wang
期刊：CNS Neuroscience & Therapeutics
影响因子 ：5.500
-
Advanced Glycation End-Products (AGEs) Promote Endothelial Cell Pyroptosis Under Cerebral Ischemia and Hypoxia via HIF-1α-RAGE-NLRP3(2023/01/18)
作者：Chenyang Han, Liping Zhai, Heping Shen
期刊：MOLECULAR NEUROBIOLOGY
DOI：10.1007/s12035-023-03228-8
影响因子 ：5.100
-
Nuclear Factor Erythroid 2-Related Factor 2 is Essential for Low-Normobaric Oxygen Treatment-Mediated Blood-Brain Barrier Protection Following Ischemic Stroke(2023/11/11)
作者：Xiaoxiao Ma, Haiyi Xie, Pinpin Hou
期刊：MOLECULAR NEUROBIOLOGY
DOI：10.1007/s12035-023-03767-0
影响因子 ：5.100
引用产品：CL-0598
-
GPR124 induces NLRP3 inflammasome-mediated pyroptosis in endothelial cells during ischemic injury(2023/12/01)
作者：Yiqian Xu, Xingyue Fang, Zhenqiang Zhao
期刊：EUROPEAN JOURNAL OF PHARMACOLOGY
DOI：10.1016/j.ejphar.2023.176228
影响因子 ：5.000
引用产品：CL-0598
-
A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost(2024/01/23)
作者：Yanghui Chen, Yuan Li, Junliang Luo
期刊：FISH & SHELLFISH IMMUNOLOGY
影响因子 ：4.700
引用产品：CL-0598
-
Vascular endothelial cell-derived exosomal miR-1246 facilitates posterior capsule opacification development by targeting GSK-3β in diabetes mellitus(2023/04/10)
作者：Chenjun Guo, Jie Zhang, Jue Wang
期刊：EXPERIMENTAL EYE RESEARCH
DOI：10.1016/j.exer.2023.109463
影响因子 ：3.400
引用产品：CL-0598
-
Nicotinamide mononucleotide (NMN) protects bEnd.3 cells against H2O2‐induced damage via NAMPT and the NF‐κB p65 signalling pathway(2021/02/09)
作者：Xiujun Deng, Xinghuan Liang, Haiyan Yang
期刊：FEBS Open Bio
影响因子 ：2.600
引用产品：CL-0598
