FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
英文名称：FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
货号： PCK005
价格： ￥5080 ￥2180 ￥1180 ￥580 ￥7480
产品信息
| 产品形态 | 液体 |
| 产品规格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 别名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 物种 | 人 |
| 表达宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 检索号 | BAG70135.1 |
| 分子量 | 17.2kDa |
| 表观分子量 | 17kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.75 ng/mL. |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 储存条件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6个月 |
| 制剂 | Supplied as a 0.2 μm filtered solution of 20 mM Citrate, 10% Trehalose, 150 mM NaCl, 0.04% PS80, 0.5 mM EDTA, pH 5.5. |
| 背景介绍 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影响因子：2.500
引用产品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子：5.600
引用产品： PC-9 细胞, A549 [A-549] 细胞, NCI-H1299 细胞, NCI-H1975 细胞, BEAS-2B 细胞, RPMI-1640 培养基, BEAS-2B细胞专用培养基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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Q1:官网的因子都是不含动物成分的么？
我们公司的因子都是重组的，是不含动物源成分的。
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Q2:细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？
如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱
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Q3:细胞因子加无菌水溶解后需要再次离心吗？
加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的
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Q4:细胞因子离心前需要放置室温吗？
不用提前放置室温.
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Q5:收到细胞因子产品用什么溶剂复融？
1、细胞因子产品大部分为冻干形式发货，少部分液体形式发货； 2、冻干粉状态的因子产品需指定溶剂复溶，请参考产品说明书【复融】方法，按照要求使用对应溶剂溶解，大部分因子为无菌水复融，少部分需要无菌水以外的特殊溶剂溶解的产品则随货会赠送相应的复融试剂；冻干粉溶解后如您需要进一步稀释分装，需使用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存。 3、液体形式因子产品不需要复溶，具体浓度详见产品标签，按照实验需要用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存即可；
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