本司产品仅用于科研,不用于临床诊断和治疗

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HEPES液(1M)

货号:PB180325

价格:¥200¥¥¥

规格:
100mL
  • 100mL
数量: - +
  • HEPES液(1M)
  • HEPES液(1M)
产品概述

HEPES全称为N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid,中文名称为4-羟乙基哌嗪乙磺酸,是一种常用的生物缓冲剂,pKa(25)=7.5,有效缓冲范围为pH6.8-8.2HEPES最大的优点是,能够在开放环境中较长时间恒定溶液的PH范围。HEPES不能通过生物膜,对生化反应影响有限,是一种化学和酶稳定的物质,而且HEPES对可见光和紫外光的吸收度极低。因此一般认为,HEPES是一种优良的生物缓冲剂,对细胞无毒性作用,使用终浓度为10-50 mM,细胞培养常用10 mM

产品信息

形态

液体

浓度

1M

规格

100mL

PH

7.2-7.4

溶剂

纯化水

HEPES

238.3g/L

储存条件

28℃或-5~-20℃,避光。

运输条件

低温

有效期

28℃,24个月;-5~-20℃,长期。

注意事项 

1、本产品经0.1μm过滤除菌;

2、使用本产品时应注意无菌操作,避免污染;

3、使用后请及时避光保存,有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢;

4、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。



参考文献
文献列表
  • The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection(2022/01/07)

    作者:Jing Jing Wang, Ying Liu, Zhou Ding

    期刊:Bioactive Materials

    DOI:10.1016/j.bioactmat.2021.12.036

    影响因子 :16.8740

    引用产品:PB180325

  • Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling(2020/09/26)

    作者:Mao Ye, Shu Dong, Haitao Hou

    期刊:Molecular Therapy-Nucleic Acids

    DOI:10.1016/j.omtn.2020.09.023

    影响因子 :10.183

    引用产品:PB180325

  • MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R(2020/01/23)

    作者:Xin Li, Jiancheng Lv, Shuai Liu

    期刊:Molecular Therapy-Nucleic Acids

    DOI:10.1016/j.omtn.2020.01.016

    影响因子 :10.183

    引用产品:PB180325

  • LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1(2021/04/06)

    作者:Kai Chen, Yifu Hou, Rui Liao

    期刊:Oncogene

    DOI:10.1038/s41388-021-01671-2

    影响因子 :8.756

    引用产品:PB180325

  • Dexmedetomidine inhibits mitochondria damage and apoptosis of enteric glial cells in experimental intestinal ischemia/reperfusion injury via SIRT3-dependent PINK1/HDAC3/p53 pathway(2021/11/12)

    作者:Qin Zhang,Xiaoming Liu, Qian Hu

    期刊:Journal of Translational Medicine

    DOI:10.1186/s12967-021-03027-6

    影响因子 :8.4400

    引用产品:PB180325

  • Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis(2021/01/20)

    作者:Liming Gao, Qi Tian,Tong Wu

    期刊:Journal of Translational Medicine

    DOI:10.1186/s12967-020-02654-9

    影响因子 :8.440

    引用产品:PB180325

  • microRNA-186 in extracellular vesicles from bone marrow mesenchymal stem cells alleviates idiopathic pulmonary fibrosis via interaction with SOX4 and DKK1(2021/02/03)

    作者:Jing Zhou, Yang Lin, Xiuhua Kang

    期刊:Stem Cell Research & Therapy

    DOI:10.1186/s13287-020-02083-x

    影响因子 :8.079

    引用产品:PB180325

  • Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis(2021/11/3)

    作者:Xiaogang Dong, Qin Pi, Anwaier Yuemaierabola

    期刊:Oxidative Medicine and Cellular Longevity

    DOI:10.1155/2021/8240015

    影响因子 :7.3100

    引用产品:PB180325

  • Oncogenic magnesium transporter 1 upregulates programmed death-1-ligand 1 expression and contributes to growth and radioresistance of glioma cells through the ERK/MAPK signaling pathway(2022/04/13)

    作者:Yuanyuan Wu, Hongbing Wang, Dongdong Wei

    期刊:Bioengineered

    DOI:10.1080/21655979.2022.2037214

    影响因子 :6.8320

    引用产品:PB180325

  • Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR(2018/12/04)

    作者:Ying Yang, Bo Gong, Zhengzheng Wu

    期刊:Journal Of Cellular Physiology

    DOI:10.1002/jcp.27837

    影响因子 :6.513

    引用产品:PB180325

  • MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis(2019/05/31)

    作者:Peng Xia, Rui Gu, Wei Zhang

    期刊:Journal Of Cellular Physiology

    DOI:10.1002/jcp.28843

    影响因子 :6.513

    引用产品:PB180325

  • Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63(2018/11/19)

    作者:Hucheng Liu, Jin Zeng, Bin Zhang

    期刊:Journal Of Cellular Physiology

    DOI:10.1002/jcp.27620

    影响因子 :6.513

    引用产品:PB180325

  • microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis(2021/03/19)

    作者:Tao Zhou, Suining Li, Liehong Yang

    期刊:Aging-US

    DOI:10.18632/aging.202721

    影响因子 :5.9550

    引用产品:PB180325

  • DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis(2021/08/24)

    作者:Zhiqiang Luo, Yanchao Ma, Tianning Di

    期刊:Aging-US

    DOI:10.18632/aging.203410

    影响因子 :5.9550

    引用产品:PB180325

  • NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock(2021/01/06)

    作者:Shisheng Wang, Chunsong Yan, Junming Luo

    期刊:Aging-US

    DOI:10.18632/aging.202379

    影响因子 :5.9550

    引用产品:PB180325

  • HBXIP promotes gastric cancer via METTL3-mediated MYC mRNA m6A modification(2020/10/13)

    作者:Zhi Yang, Xiaodi Jiang, Deming Li

    期刊:Aging-US

    DOI:10.18632/aging.103767

    影响因子 :5.9550

    引用产品:PB180325

  • microRNA-9-5p regulates the mitochondrial function of hepatocellular carcinoma cells through suppressing PDK4(2020/11/30)

    作者:Tao Si, Xuejian Ning, Hongwei Zhao

    期刊:Cancer Gene Therapy

    DOI:10.1038/s41417-020-00253-w

    影响因子 :5.854

    引用产品:PB180325

  • Environmental Factors and Their Threshold Affecting the Survival of Five Aquatic Animal Viruses in Different Animal Cells(2022/11/17)

    作者:Zi-Hao Wang, Fei Ke, Jian-Fang Gui, Qi-Ya Zhang

    期刊:Viruses-Basel

    DOI:10.3390/v14112546

    影响因子 :5.8180

    引用产品:PB180325

  • Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage via Sirtuin 5/Nrf2/HO-1 Axis(2021/06/03)

    作者:Jia Sun, Jinzhong Cai, Junhui Chen

    期刊:Front Aging Neurosci

    DOI:10.3389/fnagi.2021.646729

    影响因子 :5.702

    引用产品:PB180325

  • Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis(2021/07/10)

    作者:Yiliang Wang, Ying Zhang, Dasheng Cai

    期刊:Molecular Neurobiology

    DOI:10.1007/s12035-021-02417-7

    影响因子 :5.682

    引用产品:PB180325

  • Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis(2018/02/14)

    作者:Dongmei Wu, Shan Wang, Min Shen

    期刊:Journal Of Cellular And Molecular Medicine

    DOI:10.1111/jcmm.13532

    影响因子 :5.295

    引用产品:PB180325

  • MicroRNA-488 inhibits progression of colorectal cancer via inhibition of the mitogen-activated protein kinase pathway by targeting claudin-2(2018/09/12)

    作者:Yongbing Wang, Quan Shi, Gang Li

    期刊:AM J PHYSIOL-CELL PH

    DOI:10.1152/ajpcell.00047.2018

    影响因子 :5.2820

    引用产品:PB180325

  • Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion(2019/06/18)

    作者:Jie Shang, ZhiYong Gao, LiYan Zhang

    期刊:Cell Cycle

    DOI:10.1080/15384101.2019.1629774

    影响因子 :5.173

    引用产品:PB180325

  • Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway(2019/030/4)

    作者:Lin Chen, Rongzhen Tang, Jia Ruan

    期刊:Cell Cycle

    DOI:10.1080/15384101.2019.1578144

    影响因子 :5.173

    引用产品:PB180325

  • Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease(2021/01/07)

    作者:Yanyun Zhang, Hailan Bao, Li xia Dong

    期刊:Cell Cycle

    DOI:10.1080/15384101.2020.1863681

    影响因子 :5.173

    引用产品:PB180325

  • Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1(2020/10/16)

    作者:Lin Li, Jing Hao, Chengquan Yan

    期刊:Cell Cycle

    DOI:10.1080/15384101.2020.1823730

    影响因子 :5.173

    引用产品:PB180325

  • Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome(2021/11/11)

    作者:JunTao Wang, Peng Jiao, XiaoYing Wei

    期刊:Frontiers in Physiology

    DOI:10.3389/fphys.2021.721524

    影响因子 :4.7550

    引用产品:PB180325

  • Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury(2018/01/02)

    作者:Guihong Zheng, Xin Wen, YongJian Wang

    期刊:Journal Of Cellular Biochemistry

    DOI:10.1002/jcb.26466

    影响因子 :4.480

    引用产品:PB180325

  • LncRNA NEAT1 Inhibits Neuronal Apoptosis and Induces Neuronal Viability of Depressed Rats Via microRNA-320-3p/CRHR1 Axis(2022/01/25)

    作者:Yujing Huang, Yinshi Jin, Shuai Yao

    期刊:NEUROCHEMICAL RESEARCH

    DOI:10.1007/s11064-021-03508-6

    影响因子 :4.4140

    引用产品:PB180325

  • Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway(2018/11/1)

    作者:Dong Gao, Hongquan Chen

    期刊:American Journal of Physiology-Cell Physiology

    DOI:10.1152/ajpcell.00291.2017

    影响因子 :4.2490

    引用产品:PB180325

  • Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice(2017/8/31)

    作者:Kun Zeng, Qigao Feng, Baotao Lin

    期刊:Bioscience Reports

    DOI:10.1042/BSR20170695

    影响因子 :3.9760

    引用产品:PB180325

  • MicroRNA-23a inhibits melanoma cell proliferation, migration, and invasion in mice through a negative feedback regulation of sdcbp and the MAPK/ERK signaling pathway(2018/12/27)

    作者:Shelian Lu, Qunyuan Xu

    期刊:Iubmb Life

    DOI:10.1002/iub.1979

    影响因子 :3.8850

    引用产品:PB180325

  • miR-339-3p inhibits cell growth and epithelial–mesenchymal transition in nasopharyngeal carcinoma by modulating the KAT6A/TRIM24 axis(2022/08/17)

    作者:Pei Gao, Kun Zhao, Wuhao Lu

    期刊:International Journal of Clinical Oncology

    DOI:10.1007/s10147-022-02231-8

    影响因子 :3.8500

    引用产品:PB180325

  • Inhibition of MicroRNA-302c on Stemness of Colon Cancer Stem Cells via the CARF/Wnt/β-Catenin Axis(2020/07/02)

    作者:Yun Zhang, Hua Meng, Kun Guo

    期刊:Digestive Diseases and Sciences

    DOI:10.1007/s10620-020-06435-8

    影响因子 :3.4870

    引用产品:PB180325

  • Effects of total glucosides of paeony on acute renal injury following ischemia-reperfusion via the lncRNA HCG18/miR-16-5p/Bcl-2 axis(2022/01/11)

    作者:Wei Zhao, Yuan Zhang, Mingzhu Zhang

    期刊:Immunobiology

    DOI:10.1016/j.imbio.2022.152179

    影响因子 :3.1520

    引用产品:PB180325

  • Histone Deacetylase Sirtuin 2 Enhances Viability of Trophoblasts Through p65-Mediated MicroRNA-146a/ACKR2 Axis(2021/01/06)

    作者:Yingchun Yu, Xiaoqin An, Dongmei Fan

    期刊:Reproductive Sciences

    DOI:10.1007/s43032-020-00398-x

    影响因子 :2.9240

    引用产品:PB180325

  • Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7(2019/11/14)

    作者:Yan Zhang, Yishu Zhang, Xiaojuan Li

    期刊:Cells Tissues Organs

    DOI:10.1159/000503281

    影响因子 :2.2080

    引用产品:PB180325

  • MicroRNA-182 downregulates Wnt/β-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9(2017/09/22)

    作者:Zifeng Zhang, Yongjian Wang, Shaohua Fan

    期刊:Oncotarget

    DOI:10.18632/oncotarget.21167

    影响因子 :0.0000

    引用产品:PB180325

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