产品中心Cell Resources
联系我们CONTACT US
400-999-2100
24小时服务热线
产品概述
HEPES全称为N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid，中文名称为4-羟乙基哌嗪乙磺酸，是一种常用的生物缓冲剂，pKa(25℃)=7.5，有效缓冲范围为pH6.8-8.2。HEPES最大的优点是，能够在开放环境中较长时间恒定溶液的PH范围。HEPES不能通过生物膜，对生化反应影响有限，是一种化学和酶稳定的物质，而且HEPES对可见光和紫外光的吸收度极低。因此一般认为，HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，使用终浓度为10-50 mM，细胞培养常用10 mM。
产品信息
形态 | 液体 |
浓度 | 1M |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 超纯水 |
HEPES | 238.3g/L |
储存条件 | 2℃～8℃或-20℃，避光。 |
运输条件 | 低温 |
有效期 | 2℃～8℃，24个月；-20℃，长期。 |
注意事项
1、本产品经0.22μm过滤除菌；
2、使用本产品时应注意无菌操作，避免污染；
3、使用后请及时避光保存，有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢；
4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
-
Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling(2020/09/26)
作者：Mao Ye, Shu Dong, Haitao Hou
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.09.023
影响因子 ：10.183
引用产品：PB180325
-
MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R(2020/01/23)
作者：Xin Li, Jiancheng Lv, Shuai Liu
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.01.016
影响因子 ：10.183
引用产品：PB180325
-
LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1(2021/04/06)
作者：Kai Chen, Yifu Hou, Rui Liao
期刊：Oncogene
DOI：10.1038/s41388-021-01671-2
影响因子 ：8.756
引用产品：PB180325
-
Dexmedetomidine inhibits mitochondria damage and apoptosis of enteric glial cells in experimental intestinal ischemia/reperfusion injury via SIRT3-dependent PINK1/HDAC3/p53 pathway(2021/11/12)
作者：Qin Zhang,Xiaoming Liu, Qian Hu
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-021-03027-6
影响因子 ：8.4400
引用产品：PB180325
-
Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis(2021/01/20)
作者：Liming Gao, Qi Tian,Tong Wu
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-020-02654-9
影响因子 ：8.440
引用产品：PB180325
-
microRNA-186 in extracellular vesicles from bone marrow mesenchymal stem cells alleviates idiopathic pulmonary fibrosis via interaction with SOX4 and DKK1(2021/02/03)
作者：Jing Zhou, Yang Lin, Xiuhua Kang
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-020-02083-x
影响因子 ：8.079
引用产品：PB180325
-
Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis(2021/11/3)
作者：Xiaogang Dong, Qin Pi, Anwaier Yuemaierabola
期刊：Oxidative Medicine and Cellular Longevity
影响因子 ：7.3100
引用产品：PB180325
-
Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR(2018/12/04)
作者：Ying Yang, Bo Gong, Zhengzheng Wu
期刊：Journal Of Cellular Physiology
影响因子 ：6.513
引用产品：PB180325
-
MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis(2019/05/31)
作者：Peng Xia, Rui Gu, Wei Zhang
期刊：Journal Of Cellular Physiology
影响因子 ：6.513
引用产品：PB180325
-
Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63(2018/11/19)
作者：Hucheng Liu, Jin Zeng, Bin Zhang
期刊：Journal Of Cellular Physiology
影响因子 ：6.513
引用产品：PB180325
-
microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis(2021/03/19)
作者：Tao Zhou, Suining Li, Liehong Yang
期刊：Aging-US
影响因子 ：5.9550
引用产品：PB180325
-
DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis(2021/08/24)
作者：Zhiqiang Luo, Yanchao Ma, Tianning Di
期刊：Aging-US
影响因子 ：5.9550
引用产品：PB180325
-
NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock(2021/01/06)
作者：Shisheng Wang, Chunsong Yan, Junming Luo
期刊：Aging-US
影响因子 ：5.9550
引用产品：PB180325
-
HBXIP promotes gastric cancer via METTL3-mediated MYC mRNA m6A modification(2020/10/13)
作者：Zhi Yang, Xiaodi Jiang, Deming Li
期刊：Aging-US
影响因子 ：5.9550
引用产品：PB180325
-
microRNA-9-5p regulates the mitochondrial function of hepatocellular carcinoma cells through suppressing PDK4(2020/11/30)
作者：Tao Si, Xuejian Ning, Hongwei Zhao
期刊：Cancer Gene Therapy
DOI：10.1038/s41417-020-00253-w
影响因子 ：5.854
引用产品：PB180325
-
Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage via Sirtuin 5/Nrf2/HO-1 Axis(2021/06/03)
作者：Jia Sun, Jinzhong Cai, Junhui Chen
期刊：Front Aging Neurosci
影响因子 ：5.702
引用产品：PB180325
-
Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis(2021/07/10)
作者：Yiliang Wang, Ying Zhang, Dasheng Cai
期刊：Molecular Neurobiology
DOI：10.1007/s12035-021-02417-7
影响因子 ：5.682
引用产品：PB180325
-
Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis(2018/02/14)
作者：Dongmei Wu, Shan Wang, Min Shen
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.295
引用产品：PB180325
-
MicroRNA-488 inhibits progression of colorectal cancer via inhibition of the mitogen-activated protein kinase pathway by targeting claudin-2(2018/09/12)
作者：Yongbing Wang, Quan Shi, Gang Li
期刊：AM J PHYSIOL-CELL PH
DOI：10.1152/ajpcell.00047.2018
影响因子 ：5.2820
引用产品：PB180325
-
Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion(2019/06/18)
作者：Jie Shang, ZhiYong Gao, LiYan Zhang
期刊：Cell Cycle
DOI：10.1080/15384101.2019.1629774
影响因子 ：5.173
引用产品：PB180325
-
Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway(2019/030/4)
作者：Lin Chen, Rongzhen Tang, Jia Ruan
期刊：Cell Cycle
DOI：10.1080/15384101.2019.1578144
影响因子 ：5.173
引用产品：PB180325
-
Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease(2021/01/07)
作者：Yanyun Zhang, Hailan Bao, Li xia Dong
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1863681
影响因子 ：5.173
引用产品：PB180325
-
Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1(2020/10/16)
作者：Lin Li, Jing Hao, Chengquan Yan
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1823730
影响因子 ：5.173
引用产品：PB180325
-
Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome(2021/11/11)
作者：JunTao Wang, Peng Jiao, XiaoYing Wei
期刊：Frontiers in Physiology
影响因子 ：4.7550
引用产品：PB180325
-
Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury(2018/01/02)
作者：Guihong Zheng, Xin Wen, YongJian Wang
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.480
引用产品：PB180325
-
Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway(2018/11/1)
作者：Dong Gao, Hongquan Chen
期刊：American Journal of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00291.2017
影响因子 ：4.2490
引用产品：PB180325
-
Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice(2017/8/31)
作者：Kun Zeng, Qigao Feng, Baotao Lin
期刊：Bioscience Reports
影响因子 ：3.9760
引用产品：PB180325
-
MicroRNA-23a inhibits melanoma cell proliferation, migration, and invasion in mice through a negative feedback regulation of sdcbp and the MAPK/ERK signaling pathway(2018/12/27)
作者：Shelian Lu, Qunyuan Xu
期刊：Iubmb Life
DOI：10.1002/iub.1979
影响因子 ：3.8850
引用产品：PB180325
-
Histone Deacetylase Sirtuin 2 Enhances Viability of Trophoblasts Through p65-Mediated MicroRNA-146a/ACKR2 Axis(2021/01/06)
作者：Yingchun Yu, Xiaoqin An, Dongmei Fan
期刊：Reproductive Sciences
DOI：10.1007/s43032-020-00398-x
影响因子 ：2.9240
引用产品：PB180325
-
Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7(2019/11/14)
作者：Yan Zhang, Yishu Zhang, Xiaojuan Li
期刊：Cells Tissues Organs
影响因子 ：2.2080
引用产品：PB180325
-
MicroRNA-182 downregulates Wnt/β-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9(2017/09/22)
作者：Zifeng Zhang, Yongjian Wang, Shaohua Fan
期刊：Oncotarget
影响因子 ：0.0000
引用产品：PB180325
