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产品概述
HEPES全称为N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid，中文名称为4-羟乙基哌嗪乙磺酸，是一种常用的生物缓冲剂，pKa(25℃)=7.5，有效缓冲范围为pH6.8-8.2。HEPES最大的优点是，能够在开放环境中较长时间恒定溶液的PH范围。HEPES不能通过生物膜，对生化反应影响有限，是一种化学和酶稳定的物质，而且HEPES对可见光和紫外光的吸收度极低。因此一般认为，HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，使用终浓度为10-50 mM，细胞培养常用10 mM。
产品信息
形态 | 液体 |
浓度 | 1M |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 超纯水 |
HEPES | 238.3g/L |
储存条件 | 2℃～8℃或-20℃，避光。 |
运输条件 | 低温 |
有效期 | 2℃～8℃，24个月；-20℃，长期。 |
注意事项
1、本产品经0.22μm过滤除菌；
2、使用本产品时应注意无菌操作，避免污染；
3、使用后请及时避光保存，有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢；
4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1(2021/4/6)
作者：Kai Chen, Yifu Hou, Rui Liao
期刊：Oncogene
DOI：10.1038/s41388-021-01671-2
影响因子 ：9.867000000000001
引用产品：PB180325
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Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling(2020/9/26)
作者：Mao Ye, Shu Dong, Haitao Hou
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.09.023
影响因子 ：8.886
引用产品：PB180325
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MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R(2020/1/23)
作者：Xin Li, Jiancheng Lv, Shuai Liu
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.01.016
影响因子 ：8.886
引用产品：PB180325
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microRNA-186 in extracellular vesicles from bone marrow mesenchymal stem cells alleviates idiopathic pulmonary fibrosis via interaction with SOX4 and DKK1(2021/2/3)
作者：Jing Zhou, Yang Lin, Xiuhua Kang
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-020-02083-x
影响因子 ：6.832
引用产品：PB180325
-
Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR(2018/12/04)
作者：Ying Yang, Bo Gong, Zheng Zheng Wu
期刊：Journal Of Cellular Physiology
影响因子 ：6.384
引用产品：PB180325
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MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis(2019/5/31)
作者：Peng Xia, Rui Gu, Wei Zhang
期刊：Journal Of Cellular Physiology
影响因子 ：6.384
引用产品：PB180325
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Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63(2018/11/19)
作者：Hu Cheng Liu, Jin Zeng, Bin Zhang
期刊：Journal Of Cellular Physiology
影响因子 ：6.384
引用产品：PB180325
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microRNA-9-5p regulates the mitochondrial function of hepatocellular carcinoma cells through suppressing PDK4(2020/11/30)
作者：Si Tao, Ning Xuejian, Zhao Hongwei
期刊：Cancer Gene Therapy
DOI：10.1038/s41417-020-00253-w
影响因子 ：5.987
引用产品：PB180325
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Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage via Sirtuin 5/Nrf2/HO-1 Axis(2021/6/3)
作者：Jia Sun, Jinzhong Cai, Junhui Chen
期刊：Front Aging Neurosci
影响因子 ：5.750
引用产品：PB180325
-
DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis(2021/8/24)
作者：Zhiqiang Luo, Yanchao Ma, Tianning Di
期刊：Aging-US
影响因子 ：5.682
引用产品：PB180325
-
NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock(2021/1/6)
作者：Shisheng Wang, Chunsong Yan, Junming Luo
期刊：Aging-US
影响因子 ：5.682
引用产品：PB180325
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Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis(2021/7/10)
作者：Yi-Liang Wang, Ying Zhang, Da-Sheng Cai
期刊：Molecular Neurobiology
DOI：10.1007/s12035-021-02417-7
影响因子 ：5.59
引用产品：PB180325
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Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis(2021/1/20)
作者：Liming Gao, Qi Tian,Tong Wu
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-020-02654-9
影响因子 ：5.531
引用产品：PB180325
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Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis(2018/02/14)
作者：Dong Mei Wu, Shan Wang, Min Shen
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.31
引用产品：PB180325
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Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease(2021/01/07)
作者：Yan Yun Zhang, Hai Lan Bao, Li Xia Dong
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1863681
影响因子 ：4.534
引用产品：PB180325
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Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1(2020/10/16)
作者： Lin Li,Jing Hao ,Cheng-Quan Yan
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1823730
影响因子 ：4.534
引用产品：PB180325
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Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury(2018/01/02)
作者：Gui-Hong Zheng, Xin Wen, Yong-Jian Wang
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.429
引用产品：PB180325
