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产品概述
HEPES全称为N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid，中文名称为4-羟乙基哌嗪乙磺酸，是一种常用的生物缓冲剂，pKa(25℃)=7.5，有效缓冲范围为pH6.8-8.2。HEPES最大的优点是，能够在开放环境中较长时间恒定溶液的PH范围。HEPES不能通过生物膜，对生化反应影响有限，是一种化学和酶稳定的物质，而且HEPES对可见光和紫外光的吸收度极低。因此一般认为，HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，使用终浓度为10-50 mM，细胞培养常用10 mM。
产品信息
形态 | 液体 |
浓度 | 1M |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 纯化水 |
HEPES | 238.3g/L |
储存条件 | 2～8℃或-5~-20℃，避光。 |
运输条件 | 低温 |
有效期 | 2～8℃，24个月；-5~-20℃，长期。 |
注意事项
1、本产品经0.1μm过滤除菌；
2、使用本产品时应注意无菌操作，避免污染；
3、使用后请及时避光保存，有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢；
4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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