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产品概述
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所(Roswell Park Memorial Institute, RPMI)开发出来的,RPMI是该研究所开发的一类细胞培养基,1640是培养基代号。RPMI-1640是改进型的McCoy 's 5A培养基,使用碳酸氢盐缓冲系统,与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的,现在已广泛应用于各种正常细胞和癌细胞的培养,尤其是悬浮细胞的培养,是使用最为广泛的培养基之一。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2~7.4 |
L-谷氨酰胺 | 2mM |
NaHCO3 | 2000mg/L |
D-葡萄糖 | 2000mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 5mg/L |
储存条件 | 2℃~8℃,避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Sustained Antitumor Immunity Based on Persistent Luminescence Nanoparticles for Cancer Immunotherapy(2021/9/27)
作者:Wang Ruoping, Shi Junpeng, Song Liang
期刊:Advanced Functional Materials
影响因子 :18.808
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Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer(2021/4/8)
作者:Zhou Xiaoxiao, Liu Kun, Cui Jing
期刊:Journal of Experimental & Clinical Cancer Research
DOI:10.1186/s13046-021-01894-x
影响因子 :11.161
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Nitric oxide-releasing micelles with intelligent targeting for enhanced anti-tumor effect of cisplatin in hypoxia(2021/8/16)
作者:Yan Chen, Lei Fang, Weixin Zhou
期刊:Journal of Nanobiotechnology
DOI:10.1186/s12951-021-00989-z
影响因子 :10.435
引用产品:PM150110
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A single nanowire sensor for intracellular glucose detection(2019.06.06)
作者:Quan-Lan Liao, Hong Jiang, Xin-Wei Zhang
期刊:Nanoscale
影响因子 :7.790
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Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog‐mediated epithelial‐mesenchymal transition(2021/8/10)
作者:Guo Kaibo, Wang Peipei, Zhang Leyin
期刊:Cancer Science
影响因子 :6.716
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Development of an autophagy-related signature in pancreatic adenocarcinoma(2020/03/20)
作者:Peipei Yue, Chen Zhu, Yaxian Gao
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2020.110080
影响因子 :6.529
引用产品:PM150110
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TPGS-Modified Long-Circulating Liposomes Loading Ziyuglycoside I for Enhanced Therapy of Myelosuppression(2021/9/14)
作者:Tingting Song, Hong Wang, Yue Liu
期刊:International Journal of Nanomedicine
影响因子 :6.4
引用产品:PM150110
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FOXO3a‑SIRT6 axis suppresses aerobic glycolysis in melanoma(2020.01.17)
作者:Zhen Dong, Jie Yang, Lin Li, Li Tan
期刊:International Journal of Oncology
影响因子 :5.650
引用产品:PM150110
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者:Chao Yang, Jiang Shi, Jie Wang
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1941612
影响因子 :4.534
引用产品:164210 164210-50 CL-0165 CL-0496 CL-0519 PB180120 PM150110
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Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression(2019.08.21)
作者:Jiang-Hua Dai,
期刊:Cell Cycle
DOI:10.1080/15384101.2019.1656478
影响因子 :4.534
引用产品:PM150110
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LncRNA CCAT1 functions as apoptosis inhibitor in podocytes via autophagy inhibition(2019/08/29)
作者:Yanyan Su, Shuwen Yao, Shili Zhao
期刊:Journal Of Cellular Biochemistry
影响因子 :4.429
引用产品:PM150110
-
Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway(May 8, 2019)
作者:Zhou Zeng, Zhao‑Lin Gao, Zhi‑Pei Zhang
期刊:International Journal of Molecular Medicine
影响因子 :4.101
引用产品:PM150110
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Up-regulation of microRNA-496 suppresses proliferation, invasion, migration and in vivo tumorigenicity of human osteosarcoma cells by targeting eIF4E(2019.08)
作者:Ni-Nan Qi, Shuo Tian, Xin Li
期刊:Biochimie
DOI:10.1016/j.biochi.2019.04.017
影响因子 :4.079
引用产品:PM150110
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Synergistic effects of Rho kinase inhibitor Y-27632 and Noggin overexpression on the proliferation and neuron-like cell differentiation of stem cells derived from human exfoliated deciduous teeth.(2019.12.30)
作者:Yang S, Xin C, Zhang B, Zhang H, Hao Y
期刊:IUBMB Life
DOI:10.1002/iub.2208
影响因子 :3.885
引用产品:PM150110
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Discovery of indolyl-containing peptides as novel antibacterial agents targeting tryptophanyl-tRNA synthetase(21 Apr 2020)
作者:Shuo Zhang, Xiaodan Qiu, Ran Wang
期刊:FUTURE MEDICINAL CHEMISTRY
影响因子 :3.808
引用产品:PM150110
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Glutamine protects against oxidative stress injury through inhibiting the activation of PI3K/Akt signaling pathway in parkinsonian cell model(2019.01.05)
作者:Yingqian Zhao, Qiang Wang, Yuan Wang, Jie Li, Gang Lu & Zhibin Liu
期刊:Environmental Health and Preventive Medicine volume
影响因子 :3.674
引用产品:PM150110
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Teglicar Induces Apoptosis in HeLa Cervical Cancer Cells via Endoplasmic Reticulum Stress-Mediated Pathway(2020.07.23)
作者:Rui Zhang, Dunli Nie, Yan Chen
期刊:Iran J Sci Technol Trans Sci
DOI:10.1007/s40995-020-00940-1
影响因子 :1.194
引用产品:PM150110