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  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)

0.25%胰蛋白酶溶液(含EDTA,溶于PBS)

货号:PB180225

规格:125mL/500mL

价格: ¥68/¥228

数量: - +
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
产品概述

胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在PH 8.037℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。

一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。

产品信息

形态

液体

规格

125mL/500mL

溶剂

10mM PBS

胰蛋白酶浓度

2.5g/L

EDTA2Na

0.2g/L

酚红指示剂

储存条件

-5~-20

运输条件

冷冻

有效期

12个月

注意事项

1、由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;

2、使用本产品时应注意无菌操作,避免污染;

3、不宜长时间放置于室温环境或2~8℃长期保存;

428℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存。

5、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。

参考文献
文献列表
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    作者:Yan Liang, Wenshuai Han, Chenlu Xu

    期刊:ADVANCED FUNCTIONAL MATERIALS

    DOI:10.1002/adfm.202301256

    影响因子 :19.000

    引用产品:PB180225 PB180327 PM150210 PM150411

  • A novel pH-sensitive targeting polysaccharide-gold nanorod conjugate for combined photothermal-chemotherapy of breast cancer(2019/02/13)

    作者:Guanghui Hou, Junmin Qian, Weijun Xu

    期刊:Carbohydrate Polymers

    DOI:10.1016/j.carbpol.2019.02.045

    影响因子 :11.200

    引用产品:PB180225 PM150210B

  • Road dust exposure and human corneal damage in a plateau high geological background provincial capital city: Spatial distribution, sources, bioaccessibility, and cytotoxicity of dust heavy metals(2023/12/07)

    作者:Ziyue Yang, Hai Liu, Jingya Li

    期刊:SCIENCE OF THE TOTAL ENVIRONMENT

    DOI:10.1016/j.scitotenv.2023.169140

    影响因子 :9.800

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  • Enhanced Anticoagulation of Hierarchy Liquid Infused Surfaces in Blood Flow(2023/11/17)

    作者:Shuguang Zhang, Yuhe Wang, Kuilin Meng

    期刊:ACS Applied Materials & Interfaces

    DOI:10.1021/acsami.3c13462

    影响因子 :9.500

    引用产品:PB180225

  • A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer(2018/11/18)

    作者:Weijun Xu, Junmin Qian, Guanghui Hou

    期刊:Acta Biomaterialia

    DOI:10.1016/j.actbio.2018.11.026

    影响因子 :9.300

    引用产品:PB180225 PM150210B

  • PEGylated hydrazided gold nanorods for pH-triggered chemo/photodynamic/photothermal triple therapy of breast cancer(2018/10/12)

    作者:Weijun Xu, Junmin Qian, Guanghui Hou

    期刊:Acta Biomaterialia

    DOI:10.1016/j.actbio.2018.10.019

    影响因子 :9.300

    引用产品:PB180225 PM150210B

  • A double-network poly(Nɛ-acryloyl l-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment(2016/01/20)

    作者:Weijun Xu, Junmin Qian, Guanghui Hou

    期刊:Acta Biomaterialia

    DOI:10.1016/j.actbio.2016.01.027

    影响因子 :9.300

    引用产品:PB180225 PM150210B

  • Effects of soil-extractable metals Cd and Ni from an e-waste dismantling site on human colonic epithelial cells Caco-2: Mechanisms and implications(2021/12/17)

    作者:Jiaoyang Ma, Xinchen Bao, Wen Tian

    期刊:Chemosphere

    DOI:10.1016/j.chemosphere.2021.133361

    影响因子 :8.943

    引用产品:164210-50 PB180225 PM150411

  • Near-infrared fluorescent probes for non-invasive, real-time, and dual-color in vivo NIR-II imaging(2023/09/09)

    作者:Huixian Jia, Yujie Liu, Wei Tang

    期刊:SENSORS AND ACTUATORS B-CHEMICAL

    DOI:10.1016/j.snb.2023.134595

    影响因子 :8.400

    引用产品:164210-50 PB180120 PB180225 PM150110

  • NIR/pH dual-responsive polysaccharide-encapsulated gold nanorods for enhanced chemo-photothermal therapy of breast cancer(2019/06/03)

    作者:Weijun Xu, Jinlei Wang, Junmin Qian

    期刊:Materials Science & Engineering C-Materials for Biological Applications

    DOI:10.1016/j.msec.2019.109854

    影响因子 :7.900

    引用产品:164210 164210-50 PB180120 PB180225 PM150210

  • CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells(2023/09/29)

    作者:Hong Qin, Chenglu Sun, Dejun Kong

    期刊:Stem Cell Research & Therapy

    DOI:10.1186/s13287-023-03505-2

    影响因子 :7.500

    引用产品:PB180225

  • Near-infrared light-mediated CuS@Rutin nanocomposites for the PTT/PDT synergistic treatment in bacterial infections(2023/12/06)

    作者:Jian Liu, Baoheng Yin, Shuang Li

    期刊:JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY

    DOI:10.1016/j.jiec.2023.12.006

    影响因子 :6.100

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  • Construction of multifunctional targeted nano-prodrugs based on PAMAM dendrimers for tumor therapy(2023/10/10)

    作者:Yuan Gu, Yanjun Cai, Yige Kou

    期刊:EUROPEAN POLYMER JOURNAL

    DOI:10.1016/j.eurpolymj.2023.112486

    影响因子 :6.000

    引用产品:PB180225

  • Enhanced therapeutic efficacy of doxorubicin against multidrug-resistant breast cancer with reduced cardiotoxicity(2023/03/15)

    作者:Tianyu Zhang, Nuannuan Li, Ru Wang

    期刊:Drug Delivery

    DOI:10.1080/10717544.2023.2189118

    影响因子 :6.000

    引用产品:164210 164210-50 PB180225 PM150110

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    作者:Bingbing Zhu, Erna Jia, Qimeng Zhang

    期刊:COLLOIDS AND SURFACES B-BIOINTERFACES

    DOI:10.1016/j.colsurfb.2023.113293

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    引用产品:164210 164210-50 PB180120 PB180225 PM150421

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    期刊:International Journal Of Pharmaceutics

    DOI:10.1016/j.ijpharm.2021.121083

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    引用产品:PB180120 PB180225 PB180327

  • Hypoxia mitigation by manganese-doped carbon dots for synergistic photodynamic therapy of oral squamous cell carcinoma(2023/04/20)

    作者:Zhe Zhang, Yongzhi Xu, Tingting Zhu

    期刊:Frontiers in Bioengineering and Biotechnology

    DOI:10.3389/fbioe.2023.1153196

    影响因子 :5.700

    引用产品:164210 164210-50 P-CA-201 PB180225 PB180327 PM150210

  • PLD2 deletion alleviates disruption of tight junctions in sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway(2022/12/22)

    作者:Tiantian Qian, Boyang Qi, Yuxin Fei

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    DOI:10.1016/j.intimp.2022.109561

    影响因子 :5.600

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  • Mechanical Properties and In Vitro Biocompatibility of Hybrid Polymer-HA/BAG Ceramic Dental Materials(2022/09/09)

    作者:Yuanyuan Chen, Cheng Sun, Jinfang Cao

    期刊:Polymers

    DOI:10.3390/polym14183774

    影响因子 :5.000

    引用产品:PB180120 PB180225 PM150210

  • Screening of heat stress-regulating active fractions in mung beans(2023/02/20)

    作者:Yuchao Feng, Xia Fan, Dengcheng Suo

    期刊:Frontiers in Nutrition

    DOI:10.3389/fnut.2022.1102752

    影响因子 :5.000

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  • Angiotensin II type-2 receptor attenuates liver fibrosis progression by suppressing IRE1α-XBP1 pathway(2023/10/20)

    作者:Yue An, Changyong Xu, Wenmin Liu

    期刊:CELLULAR SIGNALLING

    DOI:10.1016/j.cellsig.2023.110935

    影响因子 :4.800

    引用产品:PB180225

  • Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro, and Anti-Bacterial Properties(2023/11/17)

    作者:Jiayu He, Yuandong Liu, Xiaofeng Zeng

    期刊:Journal of Functional Biomaterials

    DOI:10.3390/jfb14110552

    影响因子 :4.800

    引用产品:PB180225

  • Network pharmacology and experimental validation of Maxing Shigan decoction in the treatment of influenza virus-induced ferroptosis(2023/10/23)

    作者:Jiawang Huang, Xinyue Ma, Zexuan Liao

    期刊:Chinese Journal of Natural Medicines

    DOI:10.1016/S1875-5364(23)60457-1

    影响因子 :4.600

    引用产品:PB180225

  • Effect of Trimethyltin chloride on proliferation and cell cycle of intestinal porcine epithelial cells(2021/07/08)

    作者:Ying Fan, Xue Li, Qing Li 

    期刊:Comparative Biochemistry And Physiology C-Toxicology & Pharmacology

    DOI:10.1016/j.cbpc.2021.109131

    影响因子 :4.520

    引用产品:PB180120 PB180225

  • Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics(2023/11/08)

    作者:Wenxiang Deng, Qinghu He, Wenan Zhang

    期刊:NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY

    DOI:10.1007/s00210-023-02785-y

    影响因子 :3.600

    引用产品:PB180225

  • Fumonisin B1 damages the barrier functions of porcine intestinal epithelial cells in vitro(2019/09/26)

    作者:Zhigang Chen, Huiyu Chen, Xue Li

    期刊:Journal Of Biochemical And Molecular Toxicology

    DOI:10.1002/jbt.22397

    影响因子 :3.568

    引用产品:PB180120 PB180225

  • Potentilla bifurca flavonoids effectively improve insulin resistance(2022/11/30)

    作者:G.-Y. Wang, P.-Y. Yan, W. Liu, L.-K. Liu, J.-P. Li, Y. Zeng

    期刊:European Review for Medical and Pharmacological Sciences

    DOI:10.26355/eurrev_202211_30370

    影响因子 :3.300

    引用产品:CL-0006 PB180120 PB180225 PB180327 PB180432 PM150210

  • Proliferation Inhibitory Activity of Quinones from Blaps rynchopetera Defense Secretion on Colorectal Tumor Cells(2023/04/18)

    作者:Xiaoli Qian, Di Meng, Heng Liu

    期刊:Chinese Journal of Integrative Medicine

    DOI:10.1007/s11655-023-3696-y

    影响因子 :2.900

    引用产品:PB180225 PM150210 PM150411

  • TGF‑β/Smad3 pathway enhances the cardio‑protection of S1R/SIPR1 in in vitro ischemia‑reperfusion myocardial cell model(2018/7/1)

    作者:Tingfang Yang, Xianfeng Zhang, Cuimei Ma

    期刊:Experimental and Therapeutic Medicine

    DOI:10.3892/etm.2018.6192

    影响因子 :2.700

    引用产品:PB180225 PB180321

  • Downregulation of microRNA‐124 prevents the development of acute liver failure through the upregulation of PIM‐3(2020/01/01)

    作者:Tao Zan, Li Piao, Xueqin Yang

    期刊:Experimental Physiology

    DOI:10.1113/EP087963

    影响因子 :2.700

    引用产品:PB180225

  • Upregulated miR-125b mitigates inflammation, astrocyte activation, and dysfunction of spinal cord injury by inactivating the MAPK pathway.(2023/01/18)

    作者:Xianhu Yue, Mingyong Gu, Tanghong Jia

    期刊:HISTOLOGY AND HISTOPATHOLOGY

    DOI:10.14670/hh-18-624

    影响因子 :2.000

    引用产品:CM-R137 PB180225 PM150312

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