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产品概述
Dulbecco's 改良培养基--DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素的含量增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L(低糖型),后来又发展出葡萄糖含量为4500mg/L(高糖型),现已广泛应用于各种细胞的培养。
DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产,例如利用CHO细胞表达EPO和生产乙肝疫苗。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2~7.4 |
L-谷氨酰胺 | 4mM |
NaHCO3 | 3700mg/L |
D-葡萄糖 | 4500mg/L |
丙酮酸钠 | 1mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 15mg/L |
储存条件 | 2℃~8℃,避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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期刊:Cancer Letters
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影响因子 :8.679
引用产品:PM150210
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影响因子 :8.128
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影响因子 :4.315
引用产品:PM150210
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TGFβ1I1 suppressed cell migration and invasion in colorectal cancer by inhibiting the TGF-β pathway and EMT progress(2020.07)
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引用产品:PM150210
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Preparation and application of carbon dots derived from cherry blossom flowers(2019.09.16)
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期刊:Chemical Physics Letters
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影响因子 :2.328
引用产品:PM150210