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产品概述
Dulbecco's 改良培养基--DMEM（Dulbecco's Modified Eagle Medium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L（低糖型），后来又发展出葡萄糖含量为4500mg/L（高糖型），现已广泛应用于各种细胞的培养。
DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2～7.4 |
L-谷氨酰胺 | 4mM |
NaHCO3 | 3700mg/L |
D-葡萄糖 | 4500mg/L |
丙酮酸钠 | 1mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 15mg/L |
储存条件 | 2℃～8℃，避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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期刊：Journal of Experimental & Clinical Cancer Research
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Reversible regulation of SATB1 ubiquitination by USP47 and SMURF2 mediates colon cancer cell proliferation and tumor progression(2019.04.28)
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期刊：Cancer Letters
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影响因子 ：8.679
引用产品：PM150210
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影响因子 ：8.128
引用产品：PM150210
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期刊：Nanoscale
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期刊：Food Chemistry
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期刊：Frontiers in Nutrition
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引用产品：PM150210
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Inhibitory role of miR-203 in the angiogenesis of mice with pathological retinal neovascularization disease through downregulation of SNAI2(2020.07.01)
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DOI：10.1016/j.cellsig.2020.109570
影响因子 ：4.315
引用产品：PM150210
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TGFβ1I1 suppressed cell migration and invasion in colorectal cancer by inhibiting the TGF-β pathway and EMT progress(2020.07)
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影响因子 ：3.507
引用产品：PM150210
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The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta remodeling(2020.04.22)
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Preparation and application of carbon dots derived from cherry blossom flowers(2019.09.16)
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期刊：Chemical Physics Letters
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影响因子 ：2.328
引用产品：PM150210
