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产品概述
名称 | MRC-5 (人胚肺细胞) (STR鉴定正确) |
别称 | MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
生长培养基 | MEM(含NEAA)+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
注意事项 | 该细胞为有限传代细胞系,传代次数过高会导致细胞生长特性发生变化,建议到货3代内完成实验。 |
年龄(性别) | 男;14周龄 |
组织来源 | 肺 |
细胞类型 | 有限细胞系 |
生物安全等级 | 1 |
保藏机构 | ATCC; CCL-171 ECACC; 97112601 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 20 D3S1358 15,17 D5S818 11,12 D7S820 10,11 D8S1179 13 D13S317 11,14 D16S539 9,11 D18S51 15,21 D19S433 14,15 D21S11 31.2 FGA 21,23 PentaD 12 PentaE 12,16 TH01 8 TPOX 8 vWA 15 D6S1043 11,19 D12S391 20,22 D2S441 10,11 -
STR鉴定图
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参考文献
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Synthesis of selective PAK4 inhibitors for lung metastasis of lung cancer and melanoma cells(2022/03/04)
作者:Peilu Song, Fan Zhao, Dahong Li
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2022.02.029
影响因子 :14.500
引用产品:CL-0161
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Intelligent Dual-Lock Deoxyribonucleic Acid Automatons Boosting Precise Tumor Imaging(2023/01/10)
作者:Liu Wang, Kang Wang, Xiaohui Wang
期刊:ACS Applied Materials & Interfaces
影响因子 :9.500
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Interleukin-18-primed human umbilical cord-mesenchymal stem cells achieve superior therapeutic efficacy for severe viral pneumonia via enhancing T-cell immunosuppression(2023/01/28)
作者:Yan Liao, Zeqin Fu, Yinfu Huang
期刊:Cell Death & Disease
DOI:10.1038/s41419-023-05597-3
影响因子 :9.000
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Surface Engineering of HEK293 Cell-Derived Extracellular Vesicles for Improved Pharmacokinetic Profile and Targeted Delivery of IL-12 for the Treatment of Hepatocellular Carcinoma(2023/01/12)
作者:Jing Zhang, Haijing Song, Yanan Dong
期刊:International Journal of Nanomedicine
影响因子 :8.000
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Identification and validation of chemokine system-related genes in idiopathic pulmonary fibrosis(2023/04/14)
作者:Tianming Zhao, Xu Wu, Xuelei Zhao
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1159856
影响因子 :7.300
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CCL17 drives fibroblast activation in the progression of pulmonary fibrosis by enhancing the TGF-β/Smad signaling(2023/03/02)
作者:Qianrong Wang, Suosi Liu, Jiali Min
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子 :5.800
引用产品:CL-0161
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Study of the antiaging effects of birds nest peptide based on biochemical, cellular, and animal models(2023/03/05)
作者:Weijuan Bai, Xuncai Liu, Qunyan Fan
期刊:Journal of Functional Foods
影响因子 :5.600
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Circular RNA circESPL1 knockdown alleviates lipopolysaccharide (LPS)-induced lung cell injury via sponging miR-326 to regulate MAPK14(2022/08/26)
作者:Yamei Liang, Yingying Miao, Jingjing Xiang
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.109146
影响因子 :5.600
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Circ_0044411 silencing protects infantile pneumonia from lipopolysaccharide-induced cell injury by sponging miR-141-3p to inhibit CCL16 expression(2022/12/19)
作者:Yanhui Yu, Ran Wang, Hui Zhang
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.109425
影响因子 :5.600
引用产品:CL-0161
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1β-Hydroxyalantolactone from Inulae Flos alleviated the progression of pulmonary fibrosis via inhibiting JNK/FOXO1/NF-κB pathway(2021/11/07)
作者:Bing Yu, Xiaoqing Jin, Wenying Yu
期刊:International Immunopharmacology
DOI:10.1016/j.intimp.2021.108339
影响因子 :5.600
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Research into the anti-pulmonary fibrosis mechanism of Renshen Pingfei formula based on network pharmacology, metabolomics, and verification of AMPK/PPAR-γ pathway of active ingredients(2023/06/10)
作者:Lu Yan, Minyue Jiang, Xinsheng Fan
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.400
引用产品:CL-0161
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Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3(2022/11/12)
作者:Wu Xu, Zhang Hui, Jiang Gang, Peng Minlian, Li Cheng, Lu Jiaxin, Jiang Shiyin, Yang Xiaoping, Jiang Yongliang
期刊:Clinical And Experimental Immunology
影响因子 :4.600
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma(2023/02/24)
作者:Rui Chen, Zhiping Wang, Tianzhu Lu
期刊:Thoracic Cancer
影响因子 :2.900
引用产品:CL-0016 CL-0161 CL-0165 CL-0166 CL-0298 CL-0668 CM-0161
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Circ_0038467 regulates lipopolysaccharide-mediated cell proliferation, apoptosis, and inflammatory response by miR-195-5p/TLR4 axis through NF-κB pathway in MRC-5 cells(2021/03/22)
作者:Yu Zheng, Yuanyuan Wang, Xiuli Zhu
期刊:Bioscience Biotechnology and Biochemistry
影响因子 :1.600
引用产品:CL-0161
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Hsa_circ_0079530/AQP4 Axis Is Related to Non-Small Cell Lung Cancer Development and Radiosensitivity.(2022/10/01)
作者:Xianghui Yang, Min Li, Yang Zhao
期刊:Annals of Thoracic and Cardiovascular Surgery
影响因子 :1.300