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产品概述
名称 | A549 [A-549] (人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
种属 | 人 |
年龄(性别) | 男;58岁 |
组织来源 | 肺癌 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | A549细胞是由D·J·Griad通过肺癌组织移植培养建系,源自于一位58岁白人男性;A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 |
生物安全等级 | 1 |
生长培养基 | Ham's F-12K(PM150910)+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~22 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
抗原表达情况 | The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ATCC; CCL-185 ATCC; CRM-CCL-185 ATCC; CRL-7909 BCRC; 60074 BCRJ; 0033 DSMZ; ACC-107 ECACC; 86012804 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 24 D3S1358 16 D5S818 11 D7S820 8,11 D8S1179 13,14 D13S317 11 D16S539 11,12 D18S51 14,17 D19S433 13 D21S11 29 FGA 23 PentaD 9 PentaE 7,11 TH01 8,9.3 TPOX 8,11 vWA 14 D1S1656 17,18.3 D6S1043 11,13 D12S391 18 -
STR鉴定图
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参考文献
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LINC00173.v1 Promotes Angiogenesis and Progression of Lung Squamous Cell Carcinoma by Sponging miR-511-5p to Regulate VEGFA Expression(2020.07.10)
作者:Aibin Liu
期刊:Molecular Cancer
DOI:10.1186/s12943-020-01217-2
影响因子 :15.302
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20(S)-Protopanaxatriol promotes the binding of P53 and DNA to regulate the antitumor network via multiomic analysis(June 2020)
作者:Zhihua Wang, Wenbo Wu, Xiangchen Guan
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2020.01.017
影响因子 :7.097
引用产品:CL-0016
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Targeted delivery of quercetin by nanoparticles based on chitosan sensitizing paclitaxel-resistant lung cancer cells to paclitaxel()
作者:Yonghong Wang, Hongli Yu, Saisai Wang
期刊:Materials Science and Engineering: C
DOI:10.1016/j.msec.2020.111442
影响因子 :5.88
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A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells()
作者:Jin Li, Ying Gao, Haoran Guo, Xiaokang Li
期刊:Dyes and Pigments
DOI:10.1016/j.dyepig.2018.12.009
影响因子 :4.613
引用产品:CL-0016
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Optimized Fluorescent Probe for Specific Imaging of Glutathione S‐Transferases in Living Cells and Mice(2020.03.30)
作者:Tian Feng, Shouchang Gai, Haiqing Wei
期刊:Chem Asian J
影响因子 :4.056
引用产品:CL-0016
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Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer(2020.06)
作者:Fang Luo, Yi Zhao, Jiwei Liu
期刊:The International Journal of Biochemistry & Cell Biology
DOI:10.1016/j.biocel.2020.105750
影响因子 :3.673
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Elevated Notch1 enhances interleukin-22 production by CD4+ T cells via aryl hydrocarbon receptor in patients with lung adenocarcinoma(2018.11.25)
作者:Bo Pang
期刊:Bioscience Reports
影响因子 :2.942
引用产品:CL-0016
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Artesunate inhibits lung cancer cells via regulation of mitochondrial membrane potential and induction of apoptosis(2020.07.15)
作者:Yan Zhao, Jianghui Liu
期刊:Molecular Medicine Reports
影响因子 :2.1
引用产品:CL-0016
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Oxymatrine‑mediated maturation of dendritic cells leads to activation of FOXP3+/CD4+ Treg cells and reversal of cisplatin‑resistance in lung cancer cells(2019.03.20)
作者:Hui Liu Manman Zou Pei Li
期刊:Molecular Medicine Reports
影响因子 :2.1
引用产品:CL-0016