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产品概述
MEM培养基（Minimum Essential Medium）也称最低必需培养基、最小基本培养基或低限量Eagle培养基，由Harry Eagle在Eagle基本培养基（BEM）的基础上发展而来的，是一种最基本、适用范围最广的培养基，是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素，成分简单，主要用于贴壁细胞的培养，配方修改后也可用于其他类型细胞培养。
MEM含NEAA（非必须氨基酸）的培养基，是在MEM培养基的基础上添加L-丙氨酸、L-谷氨酸、L-天（门）冬酰胺、L-天（门）冬氨酸、L-脯氨酸、L-丝氨酸和甘氨酸7种NEAA，能降低细胞培养时细胞自身生产非必须氨基酸的副作用，有效促进细胞增殖代谢。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2～7.4 |
平衡盐 | Earle’s 盐 |
NEAA | 含 |
L-谷氨酰胺 | 2mM |
NaHCO3 | 2200mg/L |
D-葡萄糖 | 1000mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10mg/L |
储存条件 | 2～8℃，避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Blood–brain barrier injury and neuroinflammation induced by SARS-CoV-2 in a lung–brain microphysiological system(2023/06/22)
作者：Peng Wang, Lin Jin, Min Zhang
期刊：Nature Biomedical Engineering
DOI：10.1038/s41551-023-01054-w
影响因子 ：28.100
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Pyro-catalysis for tooth whitening via oral temperature fluctuation(2022/07/29)
作者：Yang Wang, Shuhao Wang, Yanze Meng
期刊：Nature Communications
DOI：10.1038/s41467-022-32132-3
影响因子 ：17.694
引用产品：PM150410
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m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin(2021/10/17)
作者：Diefei Liang, Weiye Lin, Meng Ren
期刊：Autophagy
DOI：10.1080/15548627.2021.1974175
影响因子 ：13.300
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ZDHHC2-mediated AGK palmitoylation activates AKT/mTOR signaling to reduce sunitinib sensitivity in renal cell carcinoma(2023/04/20)
作者：Yan Sun, Liang Zhu, Pian Liu
期刊：CANCER RESEARCH
DOI：10.1158/0008-5472.CAN-22-3105
影响因子 ：11.500
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YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway(2022/08/17)
作者：Wei Li, Kun Ye, Xurui Li
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02460-9
影响因子 ：11.300
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Shape memory and antibacterial chitosan-based cryogel with hemostasis and skin wound repair(2023/01/07)
作者：Shujun Cao, Zhanjian Bi, Qiujing Li
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.120545
影响因子 ：11.200
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New injectable chitosan-hyaluronic acid based hydrogels for hemostasis and wound healing(2022/06/23)
作者：Hongjuan Weng, Weibin Jia, Min Li
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2022.119767
影响因子 ：11.200
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Melanin-like polydopamine nanoparticles mediating anti-inflammatory and rescuing synaptic loss for inflammatory depression therapy(2023/02/10)
作者：Tingting Zhu, He Wang, Hanwen Gu
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-023-01807-4
影响因子 ：10.200
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity(2023/01/07)
作者：Xiaosong Wei, Beibei Wang, Zixin Wu
期刊：CANCER LETTERS
DOI：10.1016/j.canlet.2023.216058
影响因子 ：9.700
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作者：Meijunzi Luo, Pan Huang, Yi Pan
期刊：Cell Death & Disease
DOI：10.1038/s41419-020-03305-z
影响因子 ：9.000
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Aflatoxin B1 disrupts testicular development via the cell cycle-related Ras/PI3K/Akt signaling in mice and pig(2023/04/26)
作者：Fali Zhang, Haohai Ma, Peiyu Dong
期刊：ENVIRONMENTAL POLLUTION
DOI：10.1016/j.envpol.2023.121729
影响因子 ：8.900
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Release of antidiabetic peptides from Stichopus japonicas by simulated gastrointestinal digestion(2020/06/15)
作者：Pixian Gong, Bingkun Wang, Yanchao Wu
期刊：Food Chemistry
DOI：10.1016/j.foodchem.2020.126273
影响因子 ：8.800
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MicroRNA-10b promotes arthritis development by disrupting CD4+ T cell subtypes(2021/12/18)
作者：Jiajie Tu, Dafei Han, Yilong Fang
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DOI：10.1016/j.omtn.2021.12.022
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作者：Yi Jiang, Zongrui Luo, Yuanchao Gong
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DOI：10.1038/s41388-023-02592-y
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The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis(2023/02/02)
作者：Yapeng Wang, Mou Peng, Yawen Zhong
期刊：ONCOGENE
DOI：10.1038/s41388-023-02613-w
影响因子 ：8.000
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Piceatannol-3′-O-β-D-glucopyranoside attenuates colistin-induced neurotoxicity by suppressing oxidative stress via the NRF2/HO-1 pathway(2023/02/21)
作者：Liguang Xiong, Debiao Xiang, Fang Yuan
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114419
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Pumping-controlled multicolor modulation of upconversion emission for dual-mode dynamic anti-counterfeiting(2020/06/01)
作者：Xiaoru Dai, Ke Wang, Lei Lei
期刊：Nanophotonics
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Isoforsythiaside confers neuroprotection against Alzheimer’s disease by attenuating ferroptosis and neuroinflammation in vivo and in vitro(2023/03/21)
作者：Chunyue Wang, Hongbo Jiang, Honghan Liu
期刊：Food Science and Human Wellness
DOI：10.1016/j.fshw.2023.02.035
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期刊：Life Sciences
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FGF4 promotes skin wound repair through p38 MAPK and GSK3β-mediated stabilization of Slug(2022/12/13)
作者：Jian Sun, Jie Zhou, Jianhui Zhou
期刊：JOURNAL OF INVESTIGATIVE DERMATOLOGY
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作者：Zhiqiang Cui, Xiaoying Hu, Tuo Yang
期刊：Neural Regeneration Research
影响因子 ：6.100
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Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota(2023/01/17)
作者：Nanhai Zhang, Jingxuan Zhou, Liang Zhao
期刊：Food & Function
影响因子 ：6.100
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Novel Role of Hemeoxygenase-1 in Phthalate-Induced Renal Proximal Tubule Cell Ferroptosis(2023/01/25)
作者：Mingshan Chen, Jiaxin Wang, Hao Zhang
期刊：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
影响因子 ：6.100
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Emodin protects against lipopolysaccharide-induced inflammatory injury in HaCaT cells through upregulation of miR-21(2019/06/28)
作者：Yanping Song, Xueling Cui, Ruilan Zhao
期刊：Artificial Cells Nanomedicine and Biotechnology
DOI：10.1080/21691401.2019.1629951
影响因子 ：5.800
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Long Noncoding RNA LINC01435 Impedes Diabetic Wound Healing by Facilitating YY1-Mediated HDAC8 Expression(2021/10/27)
作者：Wan Fu, Diefei Liang, Xiaoying Wu
期刊：iScience
影响因子 ：5.800
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Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog‐mediated epithelial‐mesenchymal transition(2021/08/10)
作者：Kaibo Guo, Peipei Wang, Leyin Zhang
期刊：Cancer Science
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CircPTK2-miR-181c-5p-HMGB1: a new regulatory pathway for microglia activation and hippocampal neuronal apoptosis induced by sepsis(2021/05/05)
作者：Min Li,Junwen Hu, Yucong Peng
期刊：Molecular Medicine
DOI：10.1186/s10020-021-00305-3
影响因子 ：5.700
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Lipin2 ameliorates diabetic encephalopathy via suppressing JNK/ERK-mediated NLRP3 inflammasome overactivation(2023/03/29)
作者：Xiaochen Zhang, Shan Huang, Ziyun Zhuang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.109930
影响因子 ：5.600
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SRSF3-Mediated Ki67 Exon 7-Inclusion Promotes Head and Neck Squamous Cell Carcinoma Progression via Repressing AKR1C2(2023/02/15)
作者：Miaomiao Liu, Can Lin, Qiwei Huang
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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DOI：10.1016/j.intimp.2023.109735
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DOI：10.1016/j.intimp.2021.108393
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期刊：International Immunopharmacology
DOI：10.1016/j.intimp.2021.108338
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作者：Hancheng Wu, Qirui Hu, Ting Luo
期刊：International Immunopharmacology
DOI：10.1016/j.intimp.2021.108316
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Calycosin-7-glucoside promotes mitochondria-mediated apoptosis in hepatocellular carcinoma by targeting thioredoxin 1 to regulate oxidative stress(2023/02/21)
作者：Xiaodong Wei, Yanping Zeng, Fancheng Meng
期刊：CHEMICO-BIOLOGICAL INTERACTIONS
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DOI：10.1016/j.bbamcr.2023.119509
影响因子 ：5.100
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作者：Youhua Peng, Yujin Zhang, Meijunzi Luo
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DOI：10.1016/j.bbamcr.2023.119525
影响因子 ：5.100
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MiR-185-5p ameliorates endoplasmic reticulum stress and renal fibrosis by downregulation of ATF6(2020/06/18)
作者：Quan Yuan, Tianhua Xu, Ying Chen
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Ropivacaine suppresses tumour biological characteristics of human hepatocellular carcinoma via inhibiting IGF-1R/PI3K/AKT/mTOR signalling axis(2021/11/22)
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DOI：10.1080/21655979.2021.1995103
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Characterization of Pseudorabies Virus Associated with Severe Respiratory and Neuronal Signs in Old Pigs(2023/02/28)
作者：Hongjian Chen, Jie Fan, Xiuxiu Sun
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作者：Yueyuan Xiao, Haizhen Wang, Chang Wang
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DOI：10.1080/15384101.2020.1783934
影响因子 ：4.300
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E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1(2023/02/12)
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期刊：INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
DOI：10.1016/j.biocel.2023.106389
影响因子 ：4.000
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作者：Zhibo Yang, Zhenping Chen, Chang Wang
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DOI：10.1016/j.cyto.2021.155535
影响因子 ：3.800
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Overexpression of ARHI increases the sensitivity of cervical cancer cells to paclitaxel through inducing apoptosis and autophagy(2021/6/30)
作者：Yang Wang, Fangrong Shen, Jinhua Zhou
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MiR-19 3b regulated the formation of coat colors by targeting WNT10A and GNAI2 in Cashmere goats(2021/11/08)
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DOI：10.1080/10495398.2021.1998089
影响因子 ：3.700
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期刊：Annals of Translational Medicine
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Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1(2019/01/08)
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DOI：10.1016/j.yexmp.2019.01.003
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miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1(2020/09/01)
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期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.10.096
影响因子 ：3.100
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DOI：10.1016/j.bbrc.2022.11.035
影响因子 ：3.100
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作者：Lijuan Wang, Zhigang Chen, Dong Chen
期刊：Oncology Letters
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DOI：10.1007/s10499-023-01105-8
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Silencing of lncRNA MALAT1 inhibits cell cycle progression via androgen receptor signaling in prostate cancer cells(2019/1/7)
作者：Xiaofan Dai, Lingyun Liu, Zuowen Liang
期刊：Pathology Research And Practice
影响因子 ：2.800
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Apelin-13 attenuates early brain injury through inhibiting inflammation and apoptosis in rats after experimental subarachnoid hemorrhage(2022/01/09)
作者：Xiaoyan Shen, Guiqiang Yuan, Bing Li
期刊：Molecular Biology Reports
DOI：10.1007/s11033-021-07028-y
影响因子 ：2.800
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Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy(2021/11/19)
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期刊：Andrologia
影响因子 ：2.400
引用产品：164210 164210-50 CL-0075 CL-0143 CL-0185 CL-0200 CM-0200 PB180120 PM150110 PM150410 PM150910
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Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression(2023/02/03)
作者：Yuqian Wu, Qin Liu, Jiaojiao Tong
期刊：Molecular and Clinical Oncology
影响因子 ：1.200
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AXL upregulates c‑Myc expression through AKT and ERK signaling pathways in breast cancers(2023/02/08)
作者：Xiaobai Sun, Hong Chen, Shuling You
期刊：Molecular and Clinical Oncology
影响因子 ：1.200
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Protective Effects of Jin Bai Mei Yan Prescription on Oxidative Damage and Photoaging Induced by Ultraviolet B in HaCaT Cells(2020/06/01)
作者：NorikoMinamisawa, MiaoMing San
期刊：Digital Chinese Medicine
DOI：10.1016/j.dcmed.2020.06.001
影响因子 ：0.000
引用产品：PM150410
