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产品概述
MEM培养基(Minimum Essential Medium)也称最低必需培养基、最小基本培养基或低限量Eagle培养基,由Harry Eagle在Eagle基本培养基(BEM)的基础上发展而来的,是一种最基本、适用范围最广的培养基,是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素,成分简单,主要用于贴壁细胞的培养,配方修改后也可用于其他类型细胞培养。
MEM含NEAA(非必须氨基酸)的培养基,是在MEM培养基的基础上添加L-丙氨酸、L-谷氨酸、L-天(门)冬酰胺、L-天(门)冬氨酸、L-脯氨酸、L-丝氨酸和甘氨酸7种NEAA,能降低细胞培养时细胞自身生产非必须氨基酸的副作用,有效促进细胞增殖代谢。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2~7.4 |
平衡盐 | Earle’s 盐 |
NEAA | 含 |
L-谷氨酰胺 | 2mM |
NaHCO3 | 2200mg/L |
D-葡萄糖 | 1000mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10mg/L |
储存条件 | 2℃~8℃,避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis(2021/1/15)
作者:Meijunzi Luo, Pan Huang, Yi Pan
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-03305-z
影响因子 :8.468999999999999
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Pumping-controlled multicolor modulation of upconversion emission for dual-mode dynamic anti-counterfeiting(2020/6/1)
作者:Xiaoru Dai, Ke Wang, Lei Lei
期刊:Nanophotonics
影响因子 :8.449
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Release of antidiabetic peptides from Stichopus japonicas by simulated gastrointestinal digestion(2020.06.15)
作者:Pi-Xian Gong, Bing-Kun Wang, Yan-Chao Wu
期刊:Food Chemistry
DOI:10.1016/j.foodchem.2020.126273
影响因子 :7.514
引用产品:PM150410
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Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog‐mediated epithelial‐mesenchymal transition(2021/8/10)
作者:Guo Kaibo, Wang Peipei, Zhang Leyin
期刊:Cancer Science
影响因子 :6.716
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CircPTK2-miR-181c-5p-HMGB1: a new regulatory pathway for microglia activation and hippocampal neuronal apoptosis induced by sepsis(2021/5/05)
作者:Li Min, Hu Junwen, Peng Yucong
期刊:Molecular Medicine
DOI:10.1186/s10020-021-00305-3
影响因子 :6.354
引用产品:PM150410
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Emodin protects against lipopolysaccharide-induced inflammatory injury in HaCaT cells through upregulation of miR-21(2019.06.28)
作者:Yanping Song, Xueling Cui, Ruilan Zhao, Lanying Hu
期刊:Artif Cells Nanomed Biotechnol
DOI:10.1080/21691401.2019.1629951
影响因子 :5.678
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miR-203 promotes HaCaT cell overproliferation through targeting LXR-α and PPAR-γ(2020/06/28)
作者:Yueyuan Xiao, Haizhen Wang, Chang Wang
期刊:Cell Cycle
DOI:10.1080/15384101.2020.1783934
影响因子 :4.534
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miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1(2020.09.01)
作者:Miao Zhang Shu Zheng Yuan Haimei Sun
期刊:Journal of Tissue Engineering and Regenerative Medicine
影响因子 :3.963
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Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1(2019.01.08)
作者:Li J, Qi Y
期刊:Experimental and Molecular Pathology
DOI:10.1016/j.yexmp.2019.01.003
影响因子 :3.362
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Protective Effects of Jin Bai Mei Yan Prescription on Oxidative Damage and Photoaging Induced by Ultraviolet B in HaCaT Cells(2020.06.01)
作者:NorikoMinamisawa, MiaoMing-San
期刊:Digital Chinese Medicine
DOI:10.1016/j.dcmed.2020.06.001
影响因子 :0
引用产品:PM150410