产品中心Cell Resources
联系我们CONTACT US
400-650-3656
24小时服务热线
产品概述
名称 | HK-2 (人肾皮质近曲小管上皮细胞) (STR鉴定正确) |
别称 | Hk-2; HK2; Human Kidney-2 |
种属 | 人类 |
年龄(性别) | 成人 |
组织来源 | 肾,皮质/近端小管 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | HK-2细胞属源于正常肾的近曲小管细胞,通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2;Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317,最后将PA317细胞产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性,但未用G418筛选转导克隆。Southern和FISH分析显示,HK-2细胞来源于单克隆。PCR检测证实,HK-2细胞基因组中含有E6/E7基因。 |
生物安全等级 | 2 |
生长培养基 | MEM+10% FBS+1% P/S |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | epidermal growth factor (EGF), expressed |
基因表达情况 | alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin |
保藏机构 | ATCC; CRL-2190 BCRC; 60097 |
STR鉴定
-
STR位点信息
Amelogenin X CSF1PO 13 D2S1338 17,25 D3S1358 16,17 D5S818 12 D7S820 10,11 D8S1179 10,14 D13S317 9 D16S539 11,12 D18S51 12 D19S433 15,15.2 D21S11 28,30 FGA 20,22 PentaD 9,12 PentaE 10,11 TH01 9 TPOX 8,9 vWA 17,18 D1S1656 12,13 D6S1043 12,13 D12S391 17.3,22 -
STR鉴定图
-
参考文献
-
ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway(2020/08/22)
作者:Chen Zhida, Wu Chunying, Liu Yuting
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02912-0
影响因子 :8.469
引用产品:CL-0109
-
Liproxstatin-1 attenuates unilateral ureteral obstruction-induced renal fibrosis by inhibiting renal tubular epithelial cells ferroptosis(44450)
作者:Zhang Bo, Chen Xiang, Ru Feng
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-04137-1
影响因子 :8.468999999999999
引用产品:CL-0109
-
A calcium phosphate drug carrier loading with 5-fluorouracil achieving a synergistic effect for pancreatic cancer therapy(2021/7/20)
作者:Junzong Chen, Miaojuan Qiu, Shiqiang Zhang
期刊:Journal Of Colloid And Interface Science
DOI:10.1016/j.jcis.2021.07.080
影响因子 :8.128
引用产品:CL-0109
-
Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals(2021/9/14)
作者:Guan Linbo, Fan Ping, Liu Xinghui
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.684
-
Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress(2020/7/21)
作者:Lina Wu, Qingzhu Wang, Feng Guo
期刊:Journal Of Cellular Physiology
影响因子 :6.384
引用产品:CL-0109
-
Rapid and sensitive detection of NGAL for the prediction of acute kidney injury via a polydopamine nanosphere/aptamer nanocomplex coupled with DNase I-assisted recycling amplification(2020.04.27)
作者:Yiting Hu, Xie-an Yu, Ying Zhang, Ran Zhang, Xuefei Bai
期刊:Analyst
影响因子 :4.616
引用产品:CL-0109
-
Efficacy of Obcordata A from Aspidopterys obcordata on Kidney Stones by Inhibiting NOX4 Expression(2019.03.21)
作者:Yihang Li, Guoxu Ma, Yana Lv
期刊:Molecules
影响因子 :4.411
引用产品:CL-0109
-
Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney(2021/02/10)
作者:Yuanbin Zhang, Dongfang Liu, Fan Xue
期刊:Molecules
影响因子 :4.411
-
Rhodomyrtosone B, a membrane-targeting anti-MRSA natural acylgphloroglucinol from Rhodomyrtus tomentosa(2019.01.10)
作者:Li-Yun Zhao, Hong-Xin Liu, Lei Wang
期刊:Journal of Ethnopharmacology
影响因子 :4.360
引用产品:CL-0109
-
Drug-containing serum of rhubarb-astragalus capsule inhibits the epithelial-mesenchymal transformation of HK-2 by downregulating TGF-β1/p38MAPK/Smad2/3 pathway(2021/07/24)
作者:Meng-yuan Qin, Song-qing Huang, Xiao-qin Zou
期刊:Journal Of Ethnopharmacology
影响因子 :4.36
引用产品:CL-0109
-
Soluble (pro)renin receptor promotes fibrotic response in renal proximal tubule epithelial cells in vitro via Akt/β-catenin/Snail signaling pathway(2020.08.31)
作者:Shiying Xie, Jiahui Su, Aihua Lu
期刊:American Journal of physiology-Renal Physiology
DOI:10.1152/ajprenal.00197.2020
影响因子 :3.377
引用产品:CL-0109
-
Propofol Attenuates Hypoxia/Reoxygenation-Induced Apoptosis and Autophagy in HK-2 Cells by Inhibiting JNK Activation(2019.10.21)
作者:Huaxin Wang, Xuan Peng, Yayi Huang
期刊:Yonsei Med J
DOI:10.3349/ymj.2019.60.12.1195
影响因子 :2.759
引用产品:CL-0109