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产品概述
名称 | HK-2 (人肾皮质近曲小管上皮细胞) (STR鉴定正确) |
别称 | Hk-2; HK2; Human Kidney-2 |
背景描述 | HK-2细胞属源于正常肾的近曲小管细胞,通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2;Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317,最后将PA317细胞产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性,但未用G418筛选转导克隆。Southern和FISH分析显示,HK-2细胞来源于单克隆。PCR检测证实,HK-2细胞基因组中含有E6/E7基因。 |
种属 | 人 |
年龄(性别) | 成年 |
组织来源 | 肾,皮质/近端小管 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
细胞类型 | 转化细胞系 |
生物安全等级 | 2 |
生长培养基 | MEM(含NEAA)+10% FBS+1% P/S |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | epidermal growth factor (EGF), expressed |
基因表达情况 | alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin |
保藏机构 | ATCC; CRL-2190 BCRC; 60097 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13 D2S1338 17,25 D3S1358 16 D5S818 12 D7S820 10,11 D8S1179 10,14 D13S317 9 D16S539 12 D18S51 12 D19S433 15,15.2 D21S11 28,30 FGA 20 PentaD 9,12 PentaE 10,11 TH01 9 TPOX 8,9 vWA 17,18 D6S1043 12,13 D12S391 17.3,22 D2S441 11,12 -
STR鉴定图
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参考文献
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A Water-Soluble AIEgen for Noninvasive Diagnosis of Kidney Fibrosis via SWIR Fluorescence and Photoacoustic Imaging(2022/11/01)
作者:Dingyuan Yan, Tingting Li, Yilin Yang, Niu Niu, Deliang Wang, Jinyin Ge, Lei Wang, Ruiping Zhang, Dong Wang, Ben Zhong Tang
期刊:ADVANCED MATERIALS
影响因子 :32.0860
引用产品:CL-0109
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Adropin Carried by Reactive Oxygen Species-Responsive Nanocapsules Ameliorates Renal Lipid Toxicity in Diabetic Mice(2022/08/11)
作者:Mingchuan Yu, Di Wang, Da Zhong
期刊:ACS Applied Materials & Interfaces
影响因子 :10.3830
引用产品:CL-0109
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A calcium phosphate drug carrier loading with 5-fluorouracil achieving a synergistic effect for pancreatic cancer therapy(2021/07/20)
作者:Junzong Chen, Miaojuan Qiu, Shiqiang Zhang
期刊:Journal Of Colloid And Interface Science
DOI:10.1016/j.jcis.2021.07.080
影响因子 :9.965
引用产品:CL-0109
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ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway(2020/08/22)
作者:Zhida Chen, Chunying Wu, Yuting Liu
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02912-0
影响因子 :9.685
引用产品:CL-0109
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Liproxstatin-1 attenuates unilateral ureteral obstruction-induced renal fibrosis by inhibiting renal tubular epithelial cells ferroptosis(2021/09/11)
作者:Bo Zhang, Xiang Chen, Feng Ru
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-04137-1
影响因子 :9.685
引用产品:CL-0109
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Protective effects of Polygonatum kingianum polysaccharides and aqueous extract on uranium-induced toxicity in human kidney (HK-2) cells(2022/01/14)
作者:Wenjing Li, Libing Yu, Bo Fu
期刊:International Journal Of Biological Macromolecules
DOI:10.1016/j.ijbiomac.2022.01.043
影响因子 :8.0250
引用产品:CL-0109
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Advanced Oxidation Protein Product Promotes Oxidative Accentuation in Renal Epithelial Cells via the Soluble (Pro)renin Receptor-Mediated Intrarenal Renin-Angiotensin System and Nox4-H2O2 Signaling(2021/11/26)
作者:Kai Xue,Yurong Wang,Yan Wang
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :7.3100
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A nanosensor for precise discrimination of nephrotoxic drug mechanisms via dynamic fluorescence fingerprint strategy(2021/03/20)
作者:Xiean Yu, Xuefei Bai, Ran Zhang
期刊:Analytica Chimica Acta
影响因子 :6.911
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Chronic intermittent hypoxia induces the pyroptosis of renal tubular epithelial cells by activating the NLRP3 inflammasome(2022/03/09)
作者:Chunyan Bai, Yingfei Zhu, Qiaoliang Dong
期刊:Bioengineered
DOI:10.1080/21655979.2022.2047394
影响因子 :6.8320
引用产品:CL-0109
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Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress(2020/07/21)
作者:Lina Wu, Qingzhu Wang, Feng Guo
期刊:Journal Of Cellular Physiology
影响因子 :6.513
引用产品:CL-0109
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Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals(2021/09/14)
作者:Linbo Guan, Ping Fan, Xinghui Liu
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.081
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Calycosin attenuates renal ischemia/reperfusion injury by suppressing NF-κB mediated inflammation via PPARγ/EGR1 pathway.(2022/10/07)
作者:Ningxin Zhang, Chen Guan, Zengying Liu
期刊:Frontiers in Pharmacology
影响因子 :5.9880
引用产品:CL-0109
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Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.(2022/09/21)
作者:Longfei Yang, Yuwei Zhang, Yang Wang
期刊:Frontiers in Pharmacology
影响因子 :5.9880
引用产品:CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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T-2 Toxin Induces Kidney Fibrosis via the mtROS-NLRP3-Wnt/β-Catenin Axis(2022/10/14)
作者:Xuliang Zhang, Bo Li, Siming Huo
期刊:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
影响因子 :5.8950
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Senkyunolide I alleviates renal Ischemia-Reperfusion injury by inhibiting oxidative stress, endoplasmic reticulum stress and apoptosis(2021/11/30)
作者:Yalin Zhu, Jie Huang, Xueying Chen
期刊:International Immunopharmacology
DOI:10.1016/j.intimp.2021.108393
影响因子 :5.7140
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Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway(2020/9/9)
作者:Chunying Wu, Zhida Chen, Linlin Zhang
期刊:Hypertension Research
DOI:10.1038/s41440-020-00548-3
影响因子 :5.5280
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Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury(2021/1/19)
作者:Heyun Li, Xia Zhang, Peng Wang
期刊:Diabetology & Metabolic Syndrome
DOI:10.1186/s13098-021-00625-8
影响因子 :5.3950
引用产品:CL-0109
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Identification of inflammatory response and alternative splicing in acute kidney injury and experimental verification of the involvement of RNA‑binding protein RBFOX1 in this disease(2022/01/19)
作者:Fangyou Lin, Lei Xu, Run Yuan
期刊:INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子 :5.3140
引用产品:CL-0109
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Rapid and sensitive detection of NGAL for the prediction of acute kidney injury via a polydopamine nanosphere/aptamer nanocomplex coupled with DNase I-assisted recycling amplification(2020/04/01)
作者:Yiting Hu, Xie an Yu, Ying Zhang
期刊:Analyst
影响因子 :5.227
引用产品:CL-0109
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Rhodomyrtosone B, a membrane-targeting anti-MRSA natural acylgphloroglucinol from Rhodomyrtus tomentosa(2018/9/7)
作者:Liyun Zhao, Hongxin Liu, Lei Wang
期刊:Journal Of Ethnopharmacology
影响因子 :5.1950
引用产品:CL-0109
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Drug-containing serum of rhubarb-astragalus capsule inhibits the epithelial-mesenchymal transformation of HK-2 by downregulating TGF-β1/p38MAPK/Smad2/3 pathway(2021/07/24)
作者:Mengyuan Qin, Songqing Huang, Xiaoqin Zou
期刊:Journal Of Ethnopharmacology
影响因子 :5.195
引用产品:CL-0109
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MicroRNA 181a-2-3p Alleviates the Apoptosis of Renal Tubular Epithelial Cells via Targeting GJB2 in Sepsis-Induced Acute Kidney Injury | Molecular and Cellular Biology(2021/6/23)
作者:Huixing Yi, Shouyin Jiang, Linghua Yu
期刊:Molecular And Cellular Biology
影响因子 :5.0690
引用产品:CL-0109
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Knockdown of circ_0003928 ameliorates high glucose-induced dysfunction of human tubular epithelial cells through the miR-506-3p/ HDAC4 pathway in diabetic nephropathy(2022/04/07)
作者:Qiong Liu, Yuanyuan Cui, Nan Ding
期刊:EUROPEAN JOURNAL OF MEDICAL RESEARCH
DOI:10.1186/s40001-022-00679-y
影响因子 :4.9810
引用产品:CL-0109
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Efficacy of Obcordata A from Aspidopterys obcordata on Kidney Stones by Inhibiting NOX4 Expression(2019/03/21)
作者:Yihang Li, Guoxu Ma, Yana Lv
期刊:Molecules
影响因子 :4.927
引用产品:CL-0109
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Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney(2021/02/10)
作者:Yuanbin Zhang, Dongfang Liu, Fan Xue
期刊:Molecules
影响因子 :4.927
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Efficacy of Obcordata A from Aspidopterys obcordata on Kidney Stones by Inhibiting NOX4 Expression(2019/5/21)
作者:Yihang Li, Guoxu Ma, Yana Lv
期刊:Molecules.
DOI:10.3390/molecules24101957.
影响因子 :4.9270
引用产品:CL-0109
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The roles of ferroptosis regulatory gene SLC7A11 in renal cell carcinoma: A multi-omics study(2021/11/10)
作者:Fangshi Xu, Yibing Guan, Li Xue
期刊:Cancer Medicine
影响因子 :4.7110
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Metabolomics analysis underlay mechanisms in the renal impairment of mice caused by combination of aflatoxin M1 and ochratoxin A(2021/06/11)
作者:Ziwei Wang, Yanan Gao, Xin Huang
期刊:Toxicology
影响因子 :4.571
引用产品:CL-0109
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Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling(2021/07/15)
作者:Qiang Zheng, Hang Zhao, Dong Jia
期刊:Mediators Of Inflammation
影响因子 :4.529
引用产品:CL-0109
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HOXD1 functions as a novel tumor suppressor in kidney renal clear cell carcinoma(2021/2/19)
作者:Yuanbo Cui, Chunyan Zhang, Ya Li
期刊:Cell Biology International
影响因子 :4.4730
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δ‐Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β‐catenin nuclear localization and activating its downstream target genes(2020/1/28)
作者:Lincheng Ju, Liping Shan, Bo Yin
期刊:Cancer Medicine
影响因子 :4.4520
引用产品:CL-0109
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Comprehensive analysis of the HOXA gene family identifes HOXA13as a novel oncogenic gene in kidney renal clear cell carcinoma(2020/05/22)
作者:Yuanbo Cui, Ming Yan, Chunyan Zhang
期刊:Journal of Cancer Research and Clinical Oncology
DOI:10.1007/s00432-020-03259-x
影响因子 :4.322
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Downregulation of IRF2 Alleviates Sepsis-Related Acute Kidney Injury in vitro and in vivo(2021/12/22)
作者:Yanyan Zhang, Yun Zhang, Aixiang Yang
期刊:Drug Design Development and Therapy
影响因子 :4.3190
引用产品:CL-0109
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(Pro)renin receptor mediates albumin-induced cellular responses: role of site-1 protease-derived soluble (pro)renin receptor in renal epithelial cells(2017/12/8)
作者:Hui Fang, Chuanming Xu, Aihua Lu
期刊:Am J Physiol Cell Physiol
DOI:10.1152/ajpcell.00006.2017: 10.1152/ajpcell.00006.2017
影响因子 :4.2490
引用产品:CL-0109
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Aldosterone Induces the Proliferation of Renal Tubular Epithelial Cells In Vivo but Not In Vitro(2021/07/28)
作者:Hao Juan, Liu Lingjin, Liu Ziqian
期刊:Journal Of The Renin-Angiotensin-Aldosterone System
影响因子 :4.1090
引用产品:CL-0109
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Downregulation of circ-ZNF644 alleviates LPS-induced HK2 cell injury via miR-335-5p/HIPK1 axis(2022/09/02)
作者:Junzuo Gong, Shiqiao Zhao, Shu Luo
期刊:ENVIRONMENTAL TOXICOLOGY
影响因子 :4.1090
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Role of (Pro)Renin Receptor in Cyclosporin A-Induced Nephropathy(2022/01/24)
作者:Jiajia Hu, Yandan Tan, Yanting Chen
期刊:AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
DOI:10.1152/ajprenal.00332.2021
影响因子 :4.0970
引用产品:CL-0109
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CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via the miR-140-5p/MLKL axis(2022/08/17)
作者:Jing Xing, Songtao Fan, Hongyang Liu
期刊:JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
DOI:10.1007/s10863-022-09946-3
影响因子 :3.8530
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Soluble (pro)renin receptor promotes the fibrotic response in renal proximal tubule epithelial cells in vitro via the Akt/β-catenin/Snail signaling pathway(2020/11/1)
作者:Shiying Xie, Jiahui Su, Aihua Lu
期刊:American Journal Of Physiology-Renal Physiology
DOI:10.1152/ajprenal.00197.2020
影响因子 :3.3770
引用产品:CL-0109
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Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21–5p(2021/2/12)
作者:Wei Wei, Yuanyuan Yao, Hongyuan Bi
期刊:Biochemical And Biohysical Research Communicattons
DOI:10.1016/j.bbrc.2020.12.018.
影响因子 :3.3220
引用产品:CL-0109
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MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:(2020/09/22)
作者:H-J Wang, H Liu, Y-H Lin
期刊:Human & Experimental Toxicology
影响因子 :3.2470
引用产品:CL-0109
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Silencing circ_0074371 inhibits the progression of sepsis-induced acute kidney injury by regulating miR-330-5p/ELK1 axis(2022/08/22)
作者:Qiuyuan Wang, Rongrong Zhang, Long Cui
期刊:MAMMALIAN GENOME
DOI:10.1007/s00335-022-09961-0
影响因子 :3.2240
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High-glucose induced toxicity in HK-2 cells can be alleviated by inhibition of miRNA-320c(2022/08/15)
作者:Yan Sun, Hai Qu, Qi Song
期刊:RENAL FAILURE
DOI:10.1080/0886022X.2022.2106874
影响因子 :3.2220
引用产品:CL-0109
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High Glucose-Induced Human Kidney Cell Apoptosis and Inflammatory Injury Are Alleviated by Circ_0008529 Knockdown via Circ_0008529-Mediated miR-485-5p/WNT2B Signaling(2022/08/01)
作者:Weijun Wang, Honghua Lu
期刊:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
DOI:10.1007/s12010-022-04088-z
影响因子 :3.0940
引用产品:CL-0109
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Propofol Attenuates Hypoxia/Reoxygenation-Induced Apoptosis and Autophagy in HK-2 Cells by Inhibiting JNK Activation( 2019/11/21)
作者:Huaxin Wang, Xuan Peng, Yayi Huang
期刊:Yonsei Med J
DOI:10.3349/ymj.2019.60.12.1195
影响因子 :3.0520
引用产品:CL-0109
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Identification of hub genes and transcription factor-miRNA-mRNA pathways in mice and human renal ischemia-reperfusion injury(2021/10/26)
作者:Peng Ke, Lin Qian, Yi Zhou
期刊:PeerJ
影响因子 :2.9840
引用产品:CL-0109
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CircVMA21 ameliorates lipopolysaccharide (LPS)-induced HK-2 cell injury depending on the regulation of miR-7-5p/PPARA(2021/12/13)
作者:Fu Wang, Fangfang Zhang, Qiangqiang Tian
期刊:Autoimmunity
DOI:10.1080/08916934.2021.2012764
影响因子 :2.9570
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Thioredoxin relieves lipopolysaccharide‑induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis(2021/4/15)
作者:Jingjing Wang, Wenjuan Zhang, Guoyuan Lu
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7510
引用产品:CL-0109
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Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis(2021/05/03)
作者:Qionghua Hu, Weiwei Zen, Ming Zhang
期刊:Journal of Surgical Research
影响因子 :2.4170
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LncRNA NEAT1 knockdown ameliorates LPS-induced human kidney injury by mediating the miR-330-5p/FOXO3 axis(2022/04/01)
作者:Yi Gong, Xiao Dong, Jianjun Xu
期刊:INTERNATIONAL UROLOGY AND NEPHROLOGY
DOI:10.1007/s11255-022-03179-4
影响因子 :2.2660
引用产品:CL-0109
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Circ_0000069 contributes to the growth, metastasis and glutamine metabolism in renal cell carcinoma (RCC) via regulating miR-125a-5p-dependent SLC1A5 expression(2022/11/30)
作者:Liqing Yang, Lin Wang, Jitao Wu, Yongqiang Wang
期刊:TRANSPLANT IMMUNOLOGY
DOI:10.1016/j.trim.2022.101764
影响因子 :2.0320