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产品概述
名称 | 293 [HEK-293] (人胚肾细胞) (STR鉴定正确) |
别称 | Hek293; HEK-293; HEK 293; HEK:293; 293; 293 HEK; Human Embryonic Kidney 293 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | MEM(ATCC改良)+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
注意事项 | 该细胞贴壁松散,操作时请尽量轻柔;换液时需预热培养基;收货如有大块脱落的细胞团,为正常现象,请按照收货注意事项处理。 |
背景描述 | 293 [HEK-293]细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞,293 [HEK-293]细胞包含并表达转染的Ad5基因。早期报道中指出,293 [HEK-293]细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA,但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现,Ad5的1-4344位线性核苷酸整合入293 [HEK-293]细胞19号染色体(19q13.2)。293 [HEK-293]细胞为人类腺病毒载体扩增的宿主,可表达异常的玻连蛋白的细胞表面受体,由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 |
年龄(性别) | 胚胎 |
组织来源 | 肾;以腺病毒5DNA进行转化 |
细胞类型 | 转化细胞系 |
生物安全等级 | 2 |
倍增时间 | ~24-30 hours |
致瘤性 | Yes, forms tumors in nude mice. |
受体表达情况 | vitronectin |
保藏机构 | ATCC; CRL-1573 ATCC: PTA-4488 DSMZ: ACC-305 ECACC: 85120602 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 15,17 D5S818 8 D7S820 11,12 D8S1179 12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 15,18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鉴定图
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参考文献
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作者:Hanni Ke, Shuyan Tang, Ting Guo
期刊:NATURE MEDICINE
DOI:10.1038/s41591-022-02194-3
影响因子 :87.2410
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Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response(2022/08/05)
作者:Yecan Pan, Rui Weng, Linghao Zhang
期刊:Nano Today
DOI:10.1016/j.nantod.2022.101573
影响因子 :18.9620
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ANGPTL8 negatively regulates NF-κB activation byfacilitating selective autophagic degradation ofIKKγ(2017/12/18)
作者:Yu Zhang, Xian Guo, Wanyao Yan
期刊:Nature Communications
DOI:10.1038/s41467-017-02355-w
影响因子 :17.694
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Rational synthesis of carbon dots with phosphate ester group for direct mapping of endogenous alkaline phosphatase and polarity monitoring in living cells(2023/02/28)
作者:Yanzhi Xing, Yulong Xu, Yanna Lin
期刊:JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI:10.1016/j.jcis.2023.02.133
影响因子 :9.9650
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miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1(2022/12/30)
作者:Lixue Wang, Yuhuai Xie, Wei Chen
期刊:Cells
影响因子 :7.6660
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Silencing circular RNAPTPN12 promoted the growth of keloid fibroblasts by activating Wnt signaling pathway via targeting microRNA-21-5p(2022/01/22)
作者:Fei Liu, Tao Li, Xiaoan Zhan
期刊:Bioengineered
DOI:10.1080/21655979.2022.2029108
影响因子 :6.8320
引用产品:CL-0001
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Rapid selection of a novel GLP-1/GIP dual receptor agonist with prolonged glycemic control and weight loss in rodent animals(2020/6/26)
作者:Yumin Wu, Tiemei Ji, Jie Lv
期刊:Life Sciences
影响因子 :6.7800
引用产品:CL-0001
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Delta Opioid Peptide Targets Brain Microvascular Endothelial Cells Reducing Apoptosis to Relieve Hypoxia-Ischemic/Reperfusion Injury(2022/12/23)
作者:Ran Zhang, Meixuan Chen, Zhongfang Deng
期刊:Pharmaceutics
DOI:10.3390/pharmaceutics15010046
影响因子 :6.5250
引用产品:CL-0001
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Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans(2022/09/13)
作者:Chenghua Song, Ruichao Wen, Jiaxuan Zhou
期刊:Pharmaceutics
DOI:10.3390/pharmaceutics14091937
影响因子 :6.5250
引用产品:CL-0001
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BKCa channel participates in insulin‐induced lipid deposition in adipocytes by increasing intracellular calcium(2021/01/11)
作者:Jie Ren, Yuan Cheng, Xinxin Wen
期刊:Journal Of Cellular Physiology
影响因子 :6.513
引用产品:CL-0001
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Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals(2021/09/14)
作者:Linbo Guan, Ping Fan, Xinghui Liu
期刊:Frontiers in Cell and Developmental Biology
影响因子 :6.081
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Novel Functional Recombinant Human Follicle-Stimulating Hormone Acquired from Goat Milk(2021/03/01)
作者:Rongmao Hua, Jianxi Liu, Yuan Li
期刊:Journal Of Agricultural And Food Chemistry
影响因子 :5.8950
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Asparanin A from Asparagus officinalis L. Induces G0/G1 Cell Cycle Arrest and Apoptosis in Human Endometrial Carcinoma Ishikawa Cells via Mitochondrial and PI3K/AKT Signaling Pathways(2019/12/27)
作者:Fan Zhang, Yuanyuan Zhang, Yasai Sun
期刊:Journal Of Agricultural And Food Chemistry
影响因子 :5.895
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Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction(2018/03/06)
作者:Na Zhao, Lan Mi, Xunjun Zhang
期刊:Journal Of Molecular And Cellular Cardiology
DOI:10.1016/j.yjmcc.2018.03.006
影响因子 :5.7630
引用产品:CL-0001
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Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotropin(2022/08/31)
作者:Chunshuai Wu, Guanhua Xu, Guofeng Bao
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :5.4860
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lncRNA DGCR 5/miR‑27a‑3p/BNIP3 promotes cell apoptosis in pancreatic cancer by regulating the p38 MAPK pathway(2020/06/04)
作者:Xianjie Li, Shanxue Zhou, Tianyi Fan
期刊:International Journal of Molecular Medicine
影响因子 :5.314
引用产品:CL-0001
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Exosome‑encapsulated miR‑26a attenuates aldosterone‑induced tubulointerstitial fibrosis by inhibiting the CTGF/SMAD3 signaling pathway(2022/12/12)
作者:Hui Zheng, Jialing Ji, Tangming Zhao
期刊:INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子 :5.3140
引用产品:CL-0001
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FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice(2021/03/23)
作者:Yan Meng, Qian Yin, Qiang Ma
期刊:International Journal Of Molecular Medicine
影响因子 :5.3140
引用产品:CL-0001
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Metal-bridged DNA-functionalized silica nanoparticles for multifacet biological applications(2021/11/28)
作者:Yue Huang, Jiehua Ma, Yuting Yan
期刊:Microchemical Journal
DOI:10.1016/j.microc.2021.107017
影响因子 :5.3040
引用产品:CL-0001
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The extracellular loop of the auxiliary β1-subunit is involved in the regulation of BKCa channel mechanosensitivity(2018/9/14)
作者:Fang Xin, Yuan Cheng, Jie Ren
期刊:American Journal Of Physiology-Cell Physiology
DOI:10.1152/ajpcell.00037.2018
影响因子 :5.2820
引用产品:CL-0001
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DNA nanolantern-based split aptamer probes for in situ ATP imaging in living cells and lighting up mitochondria(2021/03/02)
作者:Yaxin Wang, Dongxia Wang, Jiayi Ma
期刊:Analyst
影响因子 :5.227
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Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro(2022/12/09)
作者:Xiaoli Li, Xiuxiu Zhang, Runhui Ma
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.1950
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Whole genome sequencing identifies a deletion mutation in the unknown-functional KCNG2 from familial sick sinus syndrome(2022/04/01)
作者:Chen Sun, Ning Li, Qianqian Wang
期刊:PHYSIOLOGICAL GENOMICS
DOI:10.1152/physiolgenomics.00132.2021
影响因子 :4.2970
引用产品:CL-0001
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Bta-miR-106b Regulates Bovine Mammary Epithelial Cell Proliferation, Cell Cycle, and Milk Protein Synthesis by Targeting the CDKN1A Gene(2022/12/07)
作者:Xin Wu, Jinfeng Huang, Yanan Liu
期刊:Genes
影响因子 :4.1410
引用产品:CL-0001
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ADAR3 alleviated inflammation and pyroptosis of neuropathic pain by targeting NLRP3 in chronic constriction injury mice(2021/08/19)
作者:Zongji Li, Jiajia Zhu, Yin Wang
期刊:Gene
DOI:10.1016/j.gene.2021.145909
影响因子 :3.913
引用产品:CL-0001
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Effects of Lycium barbarum glycopeptide on renal and testicular injury induced by di(2-ethylhexyl) phthalate(2022/04/01)
作者:Xianling Zhou, Zhigang Zhang, Heng Shi
期刊:CELL STRESS & CHAPERONES
DOI:10.1007/s12192-022-01266-0
影响因子 :3.8270
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KLF5-mediated aquaporin 3 activated autophagy to facilitate cisplatin resistance of gastric cancer(2022/09/09)
作者:Xudong Dai, Yong Chen, Ning Chen
期刊:IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
DOI:10.1080/08923973.2022.2122498
影响因子 :3.7120
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Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis(2022/12/14)
作者:Qinghua Wu, Yonggang He, Xin Liu
期刊:Regenerative Therapy
DOI:10.1016/j.reth.2022.11.005
影响因子 :3.6510
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NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24(2020/03/05)
作者:Mingsheng Chen, Yingying Cheng, Zhihai Yuan
期刊:CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影响因子 :3.632
引用产品:CL-0001
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Inhibition of Kv7/M Channel Currents by Fangchinoline(2022/12/14)
作者:Han Li, DanDan Geng, Rong Zheng
期刊:PHARMACOLOGY
影响因子 :3.4290
引用产品:CL-0001
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Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b(2022/04/07)
作者:Shuang Liang, Yushuang Sun, Lu Li
期刊:Cardiovascular Therapeutics
影响因子 :3.3680
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Preparation of porous calcium phosphate microspheres with phosphate-containing molecules at room temperature for drug delivery and osteogenic differentiation(2018/7/16)
作者:Junfeng Liu, Lu Wei, Dilixiati Duolikun
期刊:RSC Advances
影响因子 :3.3610
引用产品:CL-0001
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis(2021/6/21)
作者:Lixia Yang, Liangyan Pei, Jilong Yi
期刊:Neuropsychiatric Disease and Treatment
影响因子 :2.9890
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LncPVT1 promotes cartilage degradation in diabetic OA mice by downregulating miR-146a and activating TGF-β/SMAD4 signaling(2021/02/10)
作者:Yanzhi Wang, Yaoli, Shengkai Liang
期刊:Journal of Bone and Mineral Metabolism
DOI:10.1007/s00774-020-01199-7
影响因子 :2.9760
引用产品:CL-0001
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Circ_0116061 regulated the proliferation, apoptosis, and inflammation of osteoarthritis chondrocytes through regulating the miR-200b-3p/SMURF2 axis(2021/4/13)
作者:Wei Zheng, Guanhua Hou, Yong Li
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-021-02391-9
影响因子 :2.6770
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SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway(2023/03/17)
作者:Ping Qiu, Xing Li, Min Gong
期刊:Canadian Journal of Gastroenterology and Hepatology
影响因子 :2.6050
引用产品:164210 CL-0001 CL-0022 CL-0107 CL-0292 CL-0563 PB180120 PM150110 PM150467 PM150510 PM150810
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IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA(2023/02/21)
作者:Yujun Zhao, Xiaotie Wang, Keke Nie
期刊:TISSUE & CELL
DOI:10.1016/j.tice.2023.102046
影响因子 :2.5860
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Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis(2021/05/03)
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期刊:Journal of Surgical Research
影响因子 :2.4170
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LPS-induced PTGS2 manipulates the inflammatory response through trophoblast invasion in preeclampsia via NF-κB pathway(2022/10/31)
作者:Ruolan Li, Jirong Xie, Wei Xu
期刊:Reproductive Biology
DOI:10.1016/j.repbio.2022.100696
影响因子 :2.0890
引用产品:CL-0001
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期刊:生命科学研究
DOI:10.16605/j.cnki.1007-7847.2019.05.007
影响因子 :0
引用产品:CL-0001